Development of phosphate-affinity two-dimensional fluorescence difference gel electrophoresis
| Project/Area Number |
20790036
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
KINOSHITA Emiko Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (40379912)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | ナノ材料 / 分子認識 / 蛋白質 / バイオテクノロジー / 分析化学 / リン酸化 / フォスタグ / 電気泳動 |
|---|
| Research Abstract |
The researcher developed a novel type of two-dimensional electrophoresis using phosphate-affinity polyacrylamide gel electrophoresis (PAGE) for the analysis of phosphoproteins. By this procedure, the separations of phosphoprotein isotypes should be improved relative to those by current PAGE methods.
|
Report
(3 results)
Research Products
(52 results)
-
-
-
-
-
-
-
[Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides2009
Author(s)
T akiyama, K., Kinoshita, E., Emiko Kinoshita-Kikuta, Fujioka, Y., Kubo, Y., Koike, T
-
Journal Title
Anal. Biochem 388
Pages: 235-241
NAID
Related Report
Peer Reviewed
-
[Journal Article] Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes2009
Author(s)
K inoshita, E., Emiko Kinoshita-Kikuta, Matsubara, M., Aoki, Y., Ohie, S., Mouri, Y., Koike, T
-
Journal Title
Electrophoresis 30
Pages: 550-559
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway2008
Author(s)
I shiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Kinoshita, E., Emiko Kinoshita-Kikuta, Koike, T., Tashiro, S., Elledge, S.J., Takata, M
-
Journal Title
Nat. Struct. Mol. Biol 15
Pages: 1138-1146
Related Report
Peer Reviewed
-
-
[Journal Article] Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE2008
Author(s)
K inoshita, E., Emiko Kinoshita-Kikuta, Matsubara, M., Yamada, S., Nakamura, H., Shiro, Y., Aoki, Y., Okita, K., Koike, T
-
Journal Title
Proteomics 8
Pages: 2994-3003
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
