Structural basis of the replication-associated DNA repair by the MUTYH-PCNA complex

• Project/Area Number: 20790040
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: NAKAMURA Teruya Kumamoto University, 大学院・生命科学研究部, 助教 (40433015)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 構造生物学 / X線結晶構造解析 / DNA修復 / タンパク質複合体

Research Abstract

In order to reveal the structural insight into the replication-associated DNA repair by MUTYH and PCNA, we performed the purification and crystallization experiments for the crystal structure determination of the MUTYH-PCNA complex. We have succeeded in the purification of MUTYH and PCNA, and carried out the initial screening of crystallization conditions.

Research Products

• [Journal Article] Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base (2010)
  • Author(s): Teruya Nakamura, Sachiko Meshitsuka, Seiju Kitagawa, Nanase Abe, Junichi Yamada, Tetsuya Ishino, Hiroaki Nakano, Teruhisa Tsuzuki, Takefumi Doi, Yuji Kobayashi, Satoshi Fujii, Mutsuo Sekiguchi, Yuriko Yamagata
  • Journal Title: J. Biol. Chem. 285 Pages: 444-452
  • Peer Reviewed
• [Journal Article] Role of the glutamic acid 54 residue in transthyretin stability and thyroxine binding (2010)
  • Author(s): Masanori Miyata, Takashi Sato, Mineyuki Mizuguchi, Teruya Nakamura, Shinji Ikemizu, Yuko Nabeshima, Seiko Susuki, Yoshiaki Suwa, Hiroshi Morioka, Yukio Ando, Mary Ann Suico, Tsuyoshi Shuto, Tomoaki Koga, Yuriko Yamagata, Hirofumi Kai
  • Journal Title: Biochemistry 49 Pages: 114-123
  • Peer Reviewed
• [Journal Article] Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base (2010)
  • Author(s): Teruya Nakamura
  • Journal Title: J.Biol.Chem. 285 Pages: 444-452
  • Peer Reviewed
• [Journal Article] Role of the Glutamic Acid 54 Residue in Transthyretin Stability and Thyroxine Binding (2010)
  • Author(s): Masanori Miyata
  • Journal Title: Biochemistry 49 Pages: 114-123
  • Peer Reviewed
• [Journal Article] Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF: useful information for designing artificial proteo-antagonists (2009)
  • Author(s): Yohei Mukai, Teruya Nakamura, Yasuo Yoshioka, Hiroko Shibata, Yasuhiro Abe, Tetsuya Nomura, Madoka Taniai, Tsunetaka Ohta, Shinsaku Nakagawa, Shin-ichi Tsunoda, Haruhiko Kamada, Yuriko Yamagata, Yasuo Tsutsumi
  • Journal Title: J. Biochem. 146 Pages: 167-172
  • NAID: 10026302534
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the tumour necrosis factor alpha-tumour necrosis factor receptor type 2 complex (2009)
  • Author(s): Yohei Mukai, Teruya Nakamura, Yasuo Yoshioka, Shin-ichi Tsunoda, Haruhiko Kamada, Shinsaku Nakagawa, Yuriko Yamagata, Yasuo Tsutsumi
  • Journal Title: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 Pages: 295-298
  • Peer Reviewed
• [Journal Article] Structure-function relationship of tumor necrosis factor (TNF) and its receptor interaction based on 3D structural analysis of a fully active TNFR1-selective TNF mutant (2009)
  • Author(s): Yohei Mukai, Hiroko Shibata, Teruya Nakamura, Yasuo Yoshioka, Yasuhiro Abe, Tetsuya Nomura, Madoka Taniai, Tsunetaka Ohta, Shinji Ikemizu, Shinsaku Nakagawa, Shin-ichi Tsunoda, Haruhiko Kamada, Yuriko Yamagata, Yasuo Tsutsumi
  • Journal Title: J. Mol. Biol. 385 Pages: 1221-1229
  • Peer Reviewed
• [Journal Article] Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF : useful information for designing artificial proteo-antagonists (2009)
  • Author(s): Yohei Mukai
  • Journal Title: J.Biochem. 146 Pages: 167-172
  • Peer Reviewed
• [Journal Article] Structure-function relationship of tumor necrosis factor(TNF)and its receptor interaction based on 3D structural analysis of a fully active TNFR1-selective TN F mutant (2009)
  • Author(s): Y. Mukai
  • Journal Title: J. Mol. Biol. 385 Pages: 1221-1229
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the tumour necrosis factor alpha-tumour necrosis factor receptor type 2 complex (2009)
  • Author(s): Y. Mukai
  • Journal Title: Acta Cryst. F 65 Pages: 295-298
  • Peer Reviewed
• [Presentation] 動的タンパク質結晶学により捕らえた8-oxo-dGTPaseの求核水分子の活性化機構 (2009)
  • Author(s): 中村照也
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」, 第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター, 大阪
  • Year and Date: 2009-12-02
• [Presentation] 動的蛋白質結晶学により捕らえた8-oxo-dGTPaseの求核水分子の活性化機構 (2009)
  • Author(s): 中村照也
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-12-02
• [Presentation] Visualization of the Mn2+ dependent 8-oxo-dGTP hydrolysis reaction in E. coli MutT crystal (2009)
  • Author(s): Teruya Nakamura
  • Organizer: 第19回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 大阪大学銀杏会館, 大阪
  • Year and Date: 2009-06-12
• [Presentation] Visualization of the Mn^<2+> dependent 8-oxo-dGTP hydrolysis reaction in E.coli MutT crystal (2009)
  • Author(s): 中村照也
  • Organizer: 第19回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 大阪大学銀杏会館
  • Year and Date: 2009-06-12
• [Presentation] 機能未知であるヒト一回膜貫通型タンパク質のC末ドメインの立体構造に基づく機能予測 (2009)
  • Author(s): 中村照也
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ, 熊本
  • Year and Date: 2009-05-20
• [Presentation] ヒトMTH1の酸化ヌクレオチドに対する幅広い基質特異性の構造学的基盤 (2009)
  • Author(s): 中村照也
  • Organizer: 第26回PFシンポジウム
  • Place of Presentation: つくば国際会議場, つくば
  • Year and Date: 2009-03-25
• [Presentation] High-resolution X-ray diffraction study of the hMTH1 mutant (2008)
  • Author(s): Teruya Nakamura
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography (IUCr2008)
  • Place of Presentation: 大阪国際会議場, 大阪
• [Presentation] High-resolution X-ray diffraction study of the hMTH1 mutant (2008)
  • Author(s): 中村照也
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography(IUCr2008)
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)