STAP-2 modulates activation-induced cell death in T-cell

• Project/Area Number: 20790050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: SEKINE Yuichi Hokkaido University, 大学院・薬学研究院, 助教 (20396295)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: T細胞 / アポトーシス / AICD(活性化誘導性細胞死) / アダプター分子 / STAP-2 / 自己免疫疾患 / Caspase / シグナル伝達 / アダプタ-分子

Research Abstract

Adaptor protein STAP-2 is a multifunctional molecule which regulates cellular signaling negatively or positively in immune responses. In this study, I investigated the functional mechanisms of STAP-2 in T-cell activation-induced cell death(AICD). It is revealed that STAP-2 enhances T-cell apoptosis, so STAP-2 plays a role in T-cell AICD.

Research Products

• [Journal Article] Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1{alpha}-induced chemotaxis in T cells. (2009)
  • Author(s): Sekine, Y., O. Ikeda., S. Tsuji., C. Yamamoto, R. Muromoto, A. Nanbo, K. Oritani, A. Yoshimura, T. Matsuda
  • Journal Title: J. Immunol 183 Pages: 7966-7974
  • Peer Reviewed
• [Journal Article] The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl. (2009)
  • Author(s): Sekine, Y., C. Yamamoto, O. Ikeda, R. Muromoto, A. Nanbo, K. Oritani, A. Yoshimura, T. Matsuda
  • Journal Title: Biochem. Biophys. Res. 384 Pages: 187-192
  • NAID: 120001311780
  • Peer Reviewed
• [Journal Article] STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation. (2009)
  • Author(s): Ikeda, O., Y. Miyasaka, Y. Sekine, A. Mizushima, R. Muromoto, A. Nanbo, A. Yoshimura, T. Matsuda
  • Journal Title: Biochem. Biophys. Res. Commun 384 Pages: 71-75
  • NAID: 120001311775
  • Peer Reviewed
• [Journal Article] Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1{alpha}-induced chemotaxis in T cells (2009)
  • Author(s): Sekine, Y., et al.
  • Journal Title: J.Immunol. 183 Pages: 7966-7974
  • Peer Reviewed
• [Journal Article] The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cb1 (2009)
  • Author(s): Sekine, Y., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 384 Pages: 187-192
  • Peer Reviewed
• [Journal Article] STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation (2009)
  • Author(s): Ikeda, O., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 384 Pages: 71-75
  • Peer Reviewed
• [Journal Article] Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of STAP-2 deficient mice. Biol. Pharm. (2008)
  • Author(s): Ikeda, O., Y. Sekine, R. Muromoto, N. Ohbayashi, A. Yoshimura, T. Matsuda
  • Journal Title: Bull 31 Pages: 1790-1793
  • Peer Reviewed
• [Journal Article] STAP-2 negatively regulates both canonical and non-canonical NF-kappaB activation induced by Epstein-Barr virus-derived LMP1. Mol. (2008)
  • Author(s): Ikeda, O., Y. Sekine, T. Yasui, K. Oritani, K. Sugiyma, R. Muromoto, N. Ohbayashi, A. Yoshimura, T. Matsuda
  • Journal Title: Cell. Biol. 28 Pages: 5027-5042
  • Peer Reviewed
• [Journal Article] Enhanced c-Fms/M-CSF receptor signaling and wound-healing process in bone marrow-derived macrophages of STAP-2 deficient mice (2008)
  • Author(s): O Ikeda, et.al.
  • Journal Title: Biol. Pharm. Bull 31 Pages: 1790-1793
  • Peer Reviewed
• [Journal Article] STAP-2 negatively regulates both canonical and non-canonical NF-kappaBactivation induced by Epstein-Barr virus-derived LMP1 (2008)
  • Author(s): O Ikeda, et.al.
  • Journal Title: Mol. Cell. Biol 28 Pages: 5027-5042
  • Peer Reviewed
• [Journal Article] Physical and functional interactions between STAT3 and KAP1 (2008)
  • Author(s): R Tsuruma, et.al.
  • Journal Title: Oncogene 27 Pages: 3054-3059
  • Peer Reviewed
• [Presentation] STAP-2 modulates activation-induced cell death by influencing caspase-8 activation. (2010)
  • Author(s): Y. Sekine, C. Yamamoto, T. Matsuda
  • Organizer: Cell death pathways: Apoptosis, Autophagy and Necrosis, Keystone Symposia
  • Place of Presentation: Vancouver, British Columbia, Canada
  • Year and Date: 2010-03-15
• [Presentation] STAP-2 modulates activation-induced cell death by influencing caspase-8 activation (2010)
  • Author(s): 関根勇一, 山本千香子, 松田正
  • Organizer: Keystone Symposia
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2010-03-15
• [Presentation] STAP-2 modulates activation-induced cell death by influencing caspase-8 activation (2009)
  • Author(s): C. Yamamoto, Y. Sekine, O. Ikeda, A. Yoshiura, T. Matsuda
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] STAP-2 modulates activation-induced cell death by influencing caspase-8 activation (2009)
  • Author(s): Yamamoto, C., Y.Sekine, O.Ikeda, A.Yoshiura, T.Matsuda
  • Organizer: 第39回 日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] Physical and functional interactions among STAT3, STAP-2 and Brk (2009)
  • Author(s): Y. Miyasaka, O. Ikeda, Y. Sekine, A. Mizushima, A. Yoshimura, T. Matsuda
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] STAP-2 regulates the Brk-mediated STAT5 activation (2009)
  • Author(s): A. Mizushima, O. Ikeda, Y. Miyasaka, Y. Sekine, A. Yoshimura, T. Matsuda
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] T細胞抗原受容体(TCR)シグナルにおけるアダプター分子STAP-2の機能解析 (2009)
  • Author(s): 上野祐輔、山本千香子、関根勇一、松田正
  • Organizer: 第133回日本薬学会北海道支部例会
  • Place of Presentation: 札幌
  • Year and Date: 2009-11-28
• [Presentation] STAP-2によるAICD(activation induced cell death)制御機構の解析 (2009)
  • Author(s): 山本千香子、関根勇一、松田正
  • Organizer: 第133回日本薬学会北海道支部例会
  • Place of Presentation: 札幌
  • Year and Date: 2009-11-28
• [Presentation] STAP-2によるAICD(activation induced cell death)制御機構の解析 (2009)
  • Author(s): 山本千香子、関根勇一、松田正
  • Organizer: 第133回 日本薬学会北海道支部例会
  • Place of Presentation: 札幌
  • Year and Date: 2009-11-28
• [Presentation] 乳癌特異的チロシンキナーゼBrkとアダプター分子STAP-2の機能的相互作用の解析 (2009)
  • Author(s): 宮坂優人、池田収、水嶋明宏、関根勇一、室本竜太、南保明日香、松田正
  • Organizer: 第56回北海道薬学大会
  • Place of Presentation: 札幌
  • Year and Date: 2009-05-30
• [Presentation] STAP-2 negatively regulates both canonical and noncanonical NF-κB actibation induced by Epstein-Barr virus-derived latent membrane protein 1 (2008)
  • Author(s): O. Ikeda, Y. Sekine, M. Kakisaka, T. Yasui, A. Yoshimura, T. Matsuda
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] STAP-2 is involved in activated-induced cell death in Jurkat T cell (2008)
  • Author(s): M. Kakisaka, Y. Sekine, O. Ikeda, A. Yoshimura, T. Matsuda
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] STAP-2 is involved in activated-induced cell death in Jurkat T cell (2008)
  • Author(s): M Kakisaka, et.al.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-12-03
• [Presentation] STAP-2によるAICD(activation induced cell death)制御機構の解析 (2008)
  • Author(s): 柿坂道範、関根勇一、池田収、松田正
  • Organizer: 日本生化学会北海道支部第45回例会
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-08
• [Presentation] アダプター分子STAP-2によるEBウイルス遺伝子産物LMP1の活性制御機構の解析 (2008)
  • Author(s): 池田収、関根勇一、柿坂道範、松田正
  • Organizer: 日本生化学会北海道支部第45回例会
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-08
• [Presentation] STAP-2 regulates integrin-mediated T-cell adhesion through protein degradation of FAK (2008)
  • Author(s): T. Matsuda, O. Ikeda, Y. Sekine
  • Organizer: Experimental Biology 2008
  • Place of Presentation: San Diego, California, USA