Roles of hydrogen peroxide as intercellular signaling molecule after neural cell injury

• Project/Area Number: 20790052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: KATAYAMA Takahiro Hokkaido University, 大学院・薬学研究院, 助教 (90399957)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 過酸化水素 / 脳細胞傷害 / 脳スライス培養系 / ケモカイン / サイトカイン / ラジカル消去薬 / エダラボン / 脳保護薬 / MCP-1 / ヒドロキシラジカル / アストロサイト

Research Abstract

In rat corticostriatal slice cultures, hydrogen peroxide exposure induced upregulation of various cytokines/chemokines such as MCP-1 and IL-6 in glial cells. The effects of two free radical scavengers edaravone and dimethylthiourea on hydrogen peroxide-induced cell injury and the production of cytokines/chemokines were examined. Hydrogen peroxide-induced cell injury was inhibited by both edaravone and dimethylthiourea. On the other hand, dimethylthiourea inhibited the increased expression of cytokines/chemokines by hydrogen peroxide, whereas edaravone did not alter the expression of these cytokines/chemokines.

Research Products

• [Journal Article] Sustained activation of ERK signaling in astrocytes is critical for neuronal injury-induced monocyte chemoattractant protein-1 production in rat corticostriatal slice cultures. (2010)
  • Author(s): Katayama T., Sakaguchi E., Komatsu Y., Oguma T., Uehara T., Minami M.
  • Journal Title: Eur. J. Neurosci. 31 Pages: 1359-1367
  • Peer Reviewed
• [Journal Article] Sustained activation of ERK signaling in astrocytes is critical for neuronal injury induced monocyte chemoattractant protein-1 production in rat corticostriatal slice cultures (2010)
  • Author(s): Takahiro Katayama
  • Journal Title: Eur.J.Neurosci. 31 Pages: 1359-1367
  • Peer Reviewed
• [Journal Article] Reciprocal regulation of ATPgammaS-induced monocyte chemoattractant protein-1 by ERK and p38 MAP kinases in rat corticostriatal slice cultures. (2009)
  • Author(s): Katayama T., Ito M., Kaneko S., Satoh M., Uehara T., Minami M.
  • Journal Title: J. Neurosci. Res. 87 Pages: 1573-1581
  • Peer Reviewed
• [Journal Article] Neuronal injury induces cytokine-induced neutrophil chemoattractant-1 (CINC-1) production in astrocytes. (2009)
  • Author(s): Katayama T., Tanaka H., Yoshida T., Uehara T., Minami M.
  • Journal Title: J. Pharmacol. Sci. 109 Pages: 88-93
  • NAID: 10025733786
  • Peer Reviewed
• [Journal Article] Neuronal injury induces cytokine-induced neutrophil chemoattractant-1 (CINC-1) production in astrocytes (2009)
  • Author(s): Takahiro Katayama
  • Journal Title: J. Pharmacol. Sci 109 Pages: 88-93
  • NAID: 10025733786
  • Peer Reviewed
• [Journal Article] Reciprocal regulation of ATPyS-induced monocyte chemoattractant protein-1 by ERK and p38 MAP kinases in rat corticostriatal slice. cultures
  • Author(s): Takahiro Katayama
  • Journal Title: J. Neurosci. Res. (in press)
  • Peer Reviewed
• [Presentation] 神経細胞傷害時におけるアストロサイトでのMEK-ERK系の持続的活性化とケモカインMCP-1産生亢進 (2008)
  • Author(s): 小松陽介
  • Organizer: 第59回日本薬理学会北部会
  • Place of Presentation: 仙台(仙台市情報・産業プラザ)
  • Year and Date: 2008-09-27
• [Presentation] 過酸化水素により誘発される細胞傷害およびケモカインMCP-1産生誘導に関する検討 (2008)
  • Author(s): 小松陽介
  • Organizer: 第22回北海道薬物作用談話会
  • Place of Presentation: 札幌(北海道大学薬学部)
  • Year and Date: 2008-07-26
• [Presentation] 過酸化水素による細胞傷害およびケモカインMCP-1産生誘導に関する検討 (2008)
  • Author(s): 小松陽介
  • Organizer: 日本薬学会北海道支部第130回例会
  • Place of Presentation: 札幌(札幌コンベンションセンター)
  • Year and Date: 2008-05-10