Physiological Function of PI 3-phosphate 5-kinase

• Project/Area Number: 20790054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Akita University
• Principal Investigator: TAKASUGA Shunsuke Akita University, 大学院・医学系研究科, 助教 (90375262)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞生物学 / イノシトールリン脂質 / リン脂質 / 遺伝子改変マウス / 脂質 / 発生・分化

Research Abstract

PI 3, 5-bisphosphate is a most recently discovered PIP 2 isomer, produced via phosphorylation of PI 3-phosphate by PIP kinases. To understand the physiological function of PI 3,5-bisphosphate in adult mice, we recently generated a knockout mouse strain. Deletion of this molecule causes an excessive vacuolation in brain and a premature death. Our results indicate that this molecule is essential for central nervous system.

Research Products

• [Journal Article] The PtdIns(3, 4)P(2) phosphatase INPP4A is a suppressor of excitotoxic neuronal death (2010)
  • Author(s): Sasaki J, Kofuji S, Itoh R, Momiyama T, Takayama K, Murakami H, Chida S, Tsuya Y, Takasuga S, Eguchi S, Asanuma K, Horie Y, Miura K, Davies EM, Mitchell C, Yamazaki M, Hirai H, Takenawa T, Suzuki A, Sasaki T.
  • Journal Title: N a t u r e 465 Pages: 497-501
  • Peer Reviewed
• [Journal Article] Novel acyl-coenzyme A:monoacylglycerol acyltransferase plays an important role in hepatic triacylglycerol secretion (2010)
  • Author(s): Hiramine Y, Emoto H, Takasuga S, Hiramatsu R.
  • Journal Title: J LipidRes 51 Pages: 1424-31
  • Peer Reviewed
• [Journal Article] The PtdIns(3,4)P_2-phosphatase INPP4A is a suppressor of excitotoxic neuronal death (2010)
  • Author(s): Sasaki J., Kofuji S., Itoh R., Momiyama T., Takayama K., Murakami H., Chida S., Tsuya Y., Takasuga S, Eguchi S., Asanuma K., Horie Y., Miura K., Davies E., Mitchell C., Yamazaki M., Hirai H., Takenawa T., Suzuki A., Sasaki T.
  • Journal Title: Nature (in press)
  • Peer Reviewed
• [Journal Article] Novel acyl-coenzyme A : monoacylglycerol acyltransferase(MGAT)plays an important role in hepatic triacylglycerol secretion (2010)
  • Author(s): Hiramine Y, Emoto H, Takasuga S, Hiramatsu R.
  • Journal Title: J.Lipid Res. (in press)
  • Peer Reviewed
• [Journal Article] Mammalian phosphoinositide kinases and phosphatases. (2009)
  • Author(s): Sasaki T, Takasuga S, Sasaki J, Kofuji S, Eguchi S, Yamazaki M, Suzuki A.
  • Journal Title: Prog LipidRes 48 Pages: 307-43
  • Peer Reviewed
• [Journal Article] Sequential regulation of DOCK2 dynamics by two phospholipids during neutrophil chemotaxis. (2009)
  • Author(s): Nishikimi A, Fukuhara H, Su W, Hongu T, Takasuga S, Mihara H, Cao Q, Sanematsu F, Kanai M, Hasegawa H, Tanaka Y, Shibasaki M, Kanaho Y, Sasaki T, Frohman MA, Fukui Y.
  • Journal Title: Science 324 Pages: 384-7
  • Peer Reviewed
• [Journal Article] Mammalian phosphoinositide kinases and phosphatases (2009)
  • Author(s): Sasaki T, Takasuga S, Sasaki J, Kofuji S, Eguchi S, Yamazaki M, Suzuki A.
  • Journal Title: Prog.Lipid Res. 48 Pages: 307-343
  • Peer Reviewed
• [Journal Article] Sequential regulation of DOCK2 dynamics by two phospholipids during neutrophil chemotaxis (2009)
  • Author(s): Nishikimi A, Fukuhara H, Su W, Hongu T, Takasuga S, Mihara H, Cao Q, Sane matsu F, Kanai M, Hasegawa H, Tanaka Y, Shibasaki M, Kanaho Y, Sasaki T, Frohman MA, Fukui Y.
  • Journal Title: Science 324 Pages: 384-387
  • Peer Reviewed
• [Journal Article] Sequential Regulation of DOCK2 Dynamics by Two Phospholipids during Neutrophil Chemotaxis (2009)
  • Author(s): Nishikimi A, Fukuhara H, Su W , Hongu T, Takasuga S, Mihara H, Cao Q, Sanematsu F, Kanai M, Hasegawa H, Tanaka Y, Shi basaki M, Kanaho Y, Sasaki T, Frohman MA, Fukui Y.
  • Journal Title: Science (in press)
  • Peer Reviewed
• [Presentation] Physiological function of phosphatidylinositol 3, 5-bisphosphate in mammalian cells (2010)
  • Author(s): Takasuga S, Sasaki J, Iizuka R, Sato Y, Suzuki A, Sasaki T.
  • Organizer: The 60th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-07-01
• [Presentation] Physiological function of phosphatidylinositol -3, 5-bisphosphate in vivo (2010)
  • Author(s): Takasuga S, Sasaki J, Suzuki A, Sasaki T.
  • Organizer: The 62nd Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2010-05-19
• [Presentation] Physiological function of phosphatidylinositol 3-Phosphate 5-kinase Biochemistry and Molecular Biology (2008)
  • Author(s): Takasuga S, Iizuka R, Sato Y, Suzuki A, Sasaki T.
  • Organizer: BMB2008, The 31st Annual Meeting of the Molecular Biology Society of Japan, The 81st Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2008-12-09
• [Presentation] ホスファチジルイノシトール3リン酸5-キナーゼの生理機能解析 (2008)
  • Author(s): 高須賀俊輔、佐々木純子、飯塚了太、佐藤美子、鈴木聡、佐々木雄彦
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-09
• [Presentation] A Sac domain phosphoinositide phosphatase, Sac3 is a regulatory subunit of type III phosphatidylinositol phosphate kinase (2008)
  • Author(s): Takasuga S, Sato Y, Iizuka R, Chida S, Sasaki J, Suzuki A, Sasaki T.
  • Organizer: The 8th International Conference on Protein Phosphatases
  • Place of Presentation: Maebashi, Japan
  • Year and Date: 2008-11-12
• [Presentation] A Sac domain Phosphoinositide Phosphatase, Sac3 Is a Reguratory Sub unit of Type III Phosphatidylinositol Phosphate Kinase (2008)
  • Author(s): Takasuga S, Sato Y, Iizuka R, Chid a S, Sasaki J, Suzuki A, Sasaki T
  • Organizer: 8^<th> International Conference on Protein Phosphate ses
  • Place of Presentation: 前橋テルサ(群馬県)
  • Year and Date: 2008-11-12
• [Presentation] ホスファチジルイノシトール3リン酸5-キナーゼの生理機能 (2008)
  • Author(s): 高須賀俊輔
  • Organizer: 第2回群馬大・秋田大GCOE若手シンポジウム
  • Place of Presentation: 前橋市
  • Year and Date: 2008-07-18
• [Presentation] 哺乳動物細胞におけるホスファチジルイノシトール3, 5ニリン酸の生理機能 (2008)
  • Author(s): 高須賀俊輔、佐々木純子、飯塚了太、佐藤美子、鈴木聡、佐々木雄彦
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2008-07-01