Research on virulence regulatory mechanisms by CVF

• Project/Area Number: 20790057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: KAITO Chikara The University of Tokyo, 大学院・薬学系研究科, 講師 (60420238)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分子生物学 / 病原性 / 黄色プドウ球菌 / RNA結合タンパク質 / RNA分解酵素 / カイコ感染モデル / 黄色ブドウ球菌 / 病原性因子

Research Abstract

We previously identified the cvfA gene (SA1129) that is required for the expression of the virulence regulatory locus agr in Staphylococcus aureus using silkworm infection model. CvfA cleaves 2', 3'-cyclic phosphodiester linkage at the 3'-teminal of RNA. In this study, we performed microarray analysis of the cvfA mutant to identify the genes whose expressions are under the control of the cvfA gene. In the cvfA-deletion mutant, the expression of 20% of total genes were increased or decreased over 2-fold compared with parent strain. The WH domain is critical to CvfB function and contains a unique sequence motif. Thus CvfB represents a novel assembly of modules for binding RNA.cvfC affects the expression of metabolic genes.

Research Products

• [Journal Article] Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module. (2010)
  • Author(s): Matsumoto Y, Xu Q, Miyazaki S, Kaito C, Farr CL, Axelrod HL, Chiu HJ, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Sekimizu K, Wilson IA
  • Journal Title: Structure 18 Pages: 537-47
• [Journal Article] The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene. (2010)
  • Author(s): Ikuo M, Kaito C, Sekimizu K
  • Journal Title: Microbial Pathogenesis (in press)
• [Journal Article] The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene (2010)
  • Author(s): Ikuo M, Kaito C, Sekimizu K.
  • Journal Title: Microb Pathog. (in press)
  • Peer Reviewed
• [Journal Article] Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module (2010)
  • Author(s): Matsumoto Y, et al.(代表者は16人中4番目の著者)
  • Journal Title: Structure 18 Pages: 537-547
  • Peer Reviewed
• [Journal Article] Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity. (2009)
  • Author(s): Usui K, Miyazaki S, Kaito C, Sekimizu K
  • Journal Title: Microbial Pathogenesis 46 Pages: 59-62
• [Journal Article] Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity. (2009)
  • Author(s): Usui K, Miyazaki S, Kaito C, Sekimizu K.
  • Journal Title: Microbial Pathogenesis 46 Pages: 59-62
  • Peer Reviewed
• [Journal Article] A novel gene, fudoh, in the SCCmec region suppresses the colony spreading and virulence in Staphylococcus aureus. (2008)
  • Author(s): Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K
  • Journal Title: PLoS ONE 3
• [Journal Article] Phosphodiesterase activity of CvfA is required for virulence in Staphylococcus aureus. (2008)
  • Author(s): Nagata M, Kaito C, Sekimizu K
  • Journal Title: J Biol Chem. 283 Pages: 2176-84
• [Journal Article] Gene Expression in Pre-MBT Embryos and Activation of Maternally-Inherited Program of Apoptosis to be Executed at around MBT as a Fail-Safe Mechanism in Xenopus Early Embryogenesis. (2008)
  • Author(s): Shiokawa K, Aso M, Kondo T, Uchiyama H, Kuroyanagi S, Takai J, Takahashi S, Kajitani M, Kaito C, Sekimizu K, Takayama E, Igarashi K, Hara H
  • Journal Title: Gene Regul Syst Bio. 29,2 Pages: 213-31
• [Journal Article] A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. (2008)
  • Author(s): Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Presentation] 黄色ブドウ球菌の病原性を変化させる遺伝子 fudoh (2009)
  • Author(s): 垣内力
  • Organizer: ブドウ球菌研究会
  • Place of Presentation: 岡山
  • Year and Date: 2009-09-18
• [Presentation] A novel gene fudoh in SCCmec region regulates the colony spreading ability and virulence in Staphylococcus aureus (2008)
  • Author(s): Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K
  • Organizer: 13th International Symposium on Staphylococci and Staphylococcal Infections Cairns
  • Place of Presentation: Australia Oral presentation
• [Presentation] A Novel Gene fudoh in SCCmec Region Regulates the Colony Spreading Ability and Virulence in Staphylococcus oureus (2008)
  • Author(s): Kaito C
  • Organizer: 13th International Symposium on Staphy lococci and Staphylococcal Infections
  • Place of Presentation: オーストラリア
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~bisei/research/kaito/kaito.html
• [Remarks]
  • URL: http://read.jst.go.jp/public/fs_com_002EventAction.do?action2=event