The analysis of cellular osmotic stress response focusing on a novel kinase ASK3

• Project/Area Number: 20790058
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: NAGURO Isao The University of Tokyo, 大学院・薬学系研究科, 助教 (80401222)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ストレス応答性 / 浸透圧 / 細胞内情報伝達 / リン酸化酵素 / MAPK / ASK3 / 浸透圧ストレ / キナーゼ / 高血圧症 / ストレス応答

Research Abstract

We found that ASK3 responds to osmotic stress in the cell and alters its kinase activity by the osmotic change. We investigated the molecular mechanism how ASK3 alters the kinase activity and we suggested that ASK3 may play an important role in water and ion regulation, especially in blood pressure regulation of the body.

Research Products

• [Journal Article] The roles of ASK family proteins in stress responses and diseases. (2009)
  • Author(s): Hattori, K., Naguro, I., Runchel, C., Ichijo, H.
  • Journal Title: Cell Communication and Signaling 7 Pages: 9-9
• [Journal Article] Stress-Activated MAP Kinase Cascades in Cellular Senescence. (2009)
  • Author(s): Maruyama, J., Naguro, I., Takeda, K., Ichijo, H.
  • Journal Title: Curr. Med. Chem. 16 Pages: 1229-1235
• [Journal Article] ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis. (2009)
  • Author(s): Iriyama, T., Takeda, K., Nakamura, H.,Morimoto, Y., Kuroiwa, T., Mizukami, J.,Umeda, T., Noguchi, T., Naguro, I., Nishitoh,H., Saegusa, K., Tobiume, K., Homma, T.,Shimada, Y., Tsuda, H., Aiko, S., Imoto, I.,Inazawa, J., Chida, K., Kamei, Y., Kozuma, S.,Taketani, Y., Matsuzawa, A., Ichijo, H
  • Journal Title: EMBO J. 28 Pages: 843-853
• [Journal Article] The roles of ASK family proteins in stress responses and diseases (2009)
  • Author(s): Hattori, K., et al.
  • Journal Title: Cell Communication and Signaling 7 Pages: 9-9
  • Peer Reviewed
• [Journal Article] Stress-Activated MAP Kinase Cascades in Cellular Senescence (2009)
  • Author(s): Maruyama, J., et al.
  • Journal Title: Curr.Med.Chem. 16 Pages: 1229-1235
  • Peer Reviewed
• [Journal Article] ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis (2009)
  • Author(s): Iriyama, T., et al.
  • Journal Title: EMBO J. 28 Pages: 843-853
  • Peer Reviewed
• [Journal Article] The roles of ASK family proteins in stress responses and diseases. (2009)
  • Author(s): Hattori, K., et.al.
  • Journal Title: Cell Communication and Signaling In press
  • Peer Reviewed
• [Journal Article] Stress-Activated MAP Kinase Cascades in Cellular Senescence. (2009)
  • Author(s): Maruyama, J., et.al.
  • Journal Title: Curr. Med. Chem. 16 Pages: 1229-1235
  • Peer Reviewed
• [Journal Article] ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis. (2009)
  • Author(s): Iriyama, T., et.al.
  • Journal Title: EMBO J. 28 Pages: 843-853
  • Peer Reviewed
• [Journal Article] Requirement of reactive oxygen species-dependent activation of ASK1-p38 MAP kinase pathway for extracelluler ATP-induced apoptosis in macrophage. (2008)
  • Author(s): Noguchi, T., Ishii, K., Fukutomi, H., Naguro, I., Matsuzawa, A., Takeda, K., Ichijo, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 7657-7665
• [Journal Article] Apoptosis Signal-regulating Kinase 1 (ASK1) in stress and immune response. (2008)
  • Author(s): Takeda, K., Noguchi, T., Naguro, I., Ichijo, H.
  • Journal Title: Annu. Rev. Pharmacol. Toxicol. 48 Pages: 199-225
• [Journal Article] Requirement of reactive oxygen species-dependent activation of ASK 1-p38 MAP kinase pathway for extracelluler ATP-induced apoptosis in macrophage. (2008)
  • Author(s): Noguchi, T., et.al.
  • Journal Title: J. Biol. Chem 283 Pages: 7657-7665
  • Peer Reviewed
• [Journal Article] Apoptosis Signal-regulating Kinase 1 (ASK1) in stress and immune response. (2008)
  • Author(s): Takeda, K., et.al.
  • Journal Title: Annu. Rev. Pharmacol. Toxicol. 48 Pages: 199-225
  • Peer Reviewed
• [Journal Article] CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response.
  • Author(s): Maruyama, T., Kadowaki, H., Okamoto, N., Nagai, A., Naguro, I., Matsuzawa, A., Shibuya, H., Tanaka, K., Murata, S., Takeda, K., Nishitoh, H., Ichijo, H.
  • Journal Title: EMBO J. (in press)
• [Journal Article] ER stress-induced cell death and inflammatory signaling: a therapeutic insight into intractable diseases.
  • Author(s): Shimizu, Y., Naguro, I., Nishitoh, H., Ichijo, H.
  • Journal Title: Endoplasmic Reticulum Stress and Human Disease (in press)
• [Presentation] ASK3は浸透圧ストレスに両方向性に応答しWNK1-SPAK/OSR1経路の抑制因子として働く (2009)
  • Author(s): 名黒功、梅田剛、丸山順一、武田弘資、一條秀憲
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ASK3 bidirectinally responds to osmotic stress and negatively regulates WNK1-SPAK/OSR1 pathway (2009)
  • Author(s): Ngguro, I, Umeda, T., Maruyama, J., Ichijo, H.
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: 札幌
• [Presentation] ASK3, a novel ASK family kinase, bidirectionally responds to osmotic stress and negatively regulates WNK1-SPAK/OSR1 pathway (2009)
  • Author(s): Naguro, I., Shimizu, Y., Umeda, T., Maruyama, J., Ichijo, H.
  • Organizer: Gordon Research Conferences -Cellular Osmoregulation & Mechanotransduction-
  • Place of Presentation: USA
• [Presentation] ASK3, a novel ASK family kinase, bidirectionally responds to osmotic stress and negatively regulates WNK1-SPAK/OSR1 pathway (2009)
  • Author(s): 名黒功, et al.
  • Organizer: Gordon Research Conferences -Cellular Osmoregulation & Mechanotransduction-
  • Place of Presentation: University of NewEngland
• [Presentation] 浸透圧ストレス応答の新規シグナル伝達経路と血圧調 (2009)
  • Author(s): 名黒功, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] A novel MAP3K, ASK3 regulates WNK-SPAK/OSR1 signal transduction in osmotic stress response (2009)
  • Author(s): Naguro, I., et.al.
  • Organizer: Keystone Symposia "Mechanotransduction in Physiology and Disease"
  • Place of Presentation: Taos, New Mexico, USA
• [Presentation] 新規MAP3KであるASK3の浸透圧ストレス応答におけるWNK-SPAK/OSR1シグナル伝達経路の制御 (2008)
  • Author(s): 名黒功、橋本淳、梅田剛、武田弘資、一條秀憲
  • Organizer: BMB2008第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] ASK family kinases regulate the counteracting roles of apoptosis and inflammation in tumorigenesis (2008)
  • Author(s): Naguro, I.
  • Organizer: Gordon Research Conferences
  • Place of Presentation: USA
• [Presentation] ASK family kinases regulate the counteracting roles of apoptosis and inflammation in tumorigenesis (2008)
  • Author(s): Naguro, I., et.al.
  • Organizer: Gordon Research Conferences
  • Place of Presentation: USA, Biddefor, ME