The mechanism for endothelial cell-specific gene expression through the Robo4 promoter.
Project/Area Number |
20790064
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Biological pharmacy
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Research Institution | Osaka University |
Principal Investigator |
OKADA Yoshiaki Osaka University, 大学院・薬学研究科, 助教 (50444500)
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Project Period (FY) |
2008 – 2009
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Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | 遺伝子発現制御 / 血管内皮細胞 / Robo4遺伝子 / 組織特異的遺伝子発現 / DNAメチル化 / 組織特異性 / Hprtターゲティング法 |
Research Abstract |
To investigate the mechanism for endothelial cell-specific gene expression, the function of regulatory elements in the ROBO4 promoter were analyzed using transgenic mice. The results indicated that both regulatory elements (REn and ETS) in the upstream and proximal promoter region were contributed to the promoter activity, and that 300bp proximal promoter region was responsible for endothelial cell-specific gene expression. In addition, the study on adenovirus vector containing ROBO4 promoter-luciferase sequence demonstrated that the ROBO4 promoter maintained endothelial cell -specificity in adenovirus.
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Report
(3 results)
Research Products
(33 results)
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[Journal Article] Differential roles for ETS, CREB and EGR binding sites in mediating VEGF receptor 1 expression in vivo2009
Author(s)
E. Jin, J. Liu, J. Suehiro, L. Yaun, Y. Okada, V. Nikolova-Krstevski, K. Yano, L. Janes, D. Beeler, K.C. Spokes, D. Li, E. Regan, S.C. Shih, P. Oettgen, T. Minami, W.C. Aird
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Journal Title
Blood Vol.114
Pages: 5557-5566
Related Report
Peer Reviewed
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[Journal Article] Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR12009
Author(s)
K.H. Baek, A. Zaslavsky, R.C. Lynch, C. Britt, Y. Okada, R.J. Siarey, M.W. Lensch, I.H. Park, S.S. Yoon, T. Minami, J.R. Korenberg, J. Folkman, G.Q. Daley, W.C. Aird, Z. Galdzicki, S. Ryeom
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Journal Title
Nature Vol.459
Pages: 1126-1130
Related Report
Peer Reviewed
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[Journal Article] Elevated levels of placental growth factor represent an adaptive host response in sepsis2008
Author(s)
K. Yano, Y. Okada, G. Beldi, S.C. Shih, N. Bodyak, H. Okada, P.M. Kang, W. Luscinskas, S.C. Robson, P. Carmeliet, S.A. Karumanchi, W.C. Aird
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Journal Title
J Exp Med. Vol.205
Pages: 2623-2631
Related Report
Peer Reviewed
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[Journal Article] A GABP-binding element in the Robo4 promoter is necessary for endothelial expression in vivo2008
Author(s)
Y. Okada, E. Jin, V. Nikolova-Krstevski, K. Yano, J. Liu, D. Beeler, K. Spokes, M. Kitayama, N. Funahashi, T. Doi, L. Janes, T. Minami, P. Oettgen, W.C. Aird
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Journal Title
Blood Vol.112
Pages: 2336-2339
Related Report
Peer Reviewed
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[Journal Article] Ligand-independent assembly of purified soluble magic roundabout (Robo4), a tumor-specific endothelial marker2008
Author(s)
M. Yoshikawa, Y. Mukai, Y. Okada, Y. Yoshioka, S. Tsunoda, Y. Tsutsumi, N. Okada, W.C. Aird, T. Doi, S. Nakagawa
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Journal Title
Protein Expr Purif. Vol.61
Pages: 78-82
Related Report
Peer Reviewed
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[Journal Article] Ligand-independent assembly of purified soluble magic roundabout (Robo4), a tumor-specific endothelial marker2008
Author(s)
Yoshikawa M, Mukai Y, Okada Y, Yoshioka Y, Tsunoda S, Tsutsumi Y, Okada N, Aird WC, Doi T, Nakagawa S.
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Journal Title
Protein Expr Purif 61
Pages: 78-82
Related Report
Peer Reviewed
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[Journal Article] A GABP-binding element in the Robo4 promoter is necessary for endothelial expression in vivo2008
Author(s)
Okada Y, Jin E, Nikolova-Krstevski V, Yano K, Liu J, Beeler D, Spokes K, Kitayama M, Funahashi N, Doi T, Janes L, Minami T, Oettgen P, Aird WC.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Elevated levels of placental growth factor represent an adaptive host response in sepsis2008
Author(s)
Yano K, Okada Y, Beldi G, Shih SC, Bodyak N, Okada H, Kang PM, Luscinskas W, Robson SC, Carmeliet P, Karumanchi SA, Aird WC.
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Journal Title
J Exp Med 205
Pages: 2623-31
Related Report
Peer Reviewed
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