Molecular Basis of the regulation of ETS transcription factor MEF expression by tumor suppressor gene p53

• Project/Area Number: 20790068
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: ANN SOTEN Suico mary Kumamoto University, 大学院・生命科学研究部, 助教 (20363525)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 薬理学 / MEF / ETS / p53 / E2F / p21 / Myeloid elf-1 like factor (MEF) / Sp1 / E2F1 / プロモーター / 転写 / Meloid elf-1 like factor(MEF)

Research Abstract

MEF is one of the ETS transcription factors which regulates a diverse range of innate immune molecules including antibacterial peptides, cytokines and innate immune cell progression. In the present study, we investigated the role of tumor suppressor gene p53 in the regulation of MEF expression. We demonstrate that p53 directly binds to E2F1 and inhibits E2F1 binding to MEF promoter, thereby suppressing MEF transcription.

Research Products

• [Journal Article] Role of the glutamic acid 54 residue in transthyretin stability and thyroxine binding (2010)
  • Author(s): Miyata M, Sato T, Mizuguchi M, Nakamura T, Ikemizu S, Nabeshima Y, Susuki S, Suwa Y, Morioka H, Ando Y, Suico MA, Shuto T, Koga T, Yamagata Y, Kai H.
  • Journal Title: Biochemistry 49 Pages: 114-23
  • Peer Reviewed
• [Journal Article] TLR3 induction by anticancer drugs potentiates poly I:C-induced tumor cell apoptosis (2010)
  • Author(s): Manabu Taura, R. Fukuda, Mary Ann Suico, Ayaka Eguma, Tomoaki Koga, Tsuyoshi Shuto, Takashi Sato, Saori Koga Morino, Hirofumi Kai
  • Journal Title: Cancer Sci (in press)
  • Peer Reviewed
• [Journal Article] Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury (2010)
  • Author(s): Mariko Oba, Mary Ann Suico, Saori Morino, Shuichiro Yano, Takashi Matsuno, Tomoaki Koga, Takashi Sato, Tsuyoshi Shuto, Hirofumi Kai
  • Journal Title: J. Surg. Res. (in press)
  • Peer Reviewed
• [Journal Article] TLR3 induction by anticancer drugs potentiates poly I : C-induced tumor cell apoptosis. (2010)
  • Author(s): Taura M, et al.
  • Journal Title: Cancer Sci. (in press)
  • Peer Reviewed
• [Journal Article] The endoplasmic reticulum-associated degradation of transthyretin variants is negatively regulated by BiP in mammalian cells (2009)
  • Author(s): Susuki S, Sato T, Miyata M, Momohara M, Suico MA, Shuto T, Ando Y, Kai H.
  • Journal Title: J Biol Chem. 284 Pages: 8312-8321
  • Peer Reviewed
• [Journal Article] Calreticulin positively regulates the expression and function of epithelial sodium channel (2009)
  • Author(s): Takuya Sugahara, Tomoaki Koga, Keiko Ueno-Shuto, Tsuyoshi Shuto, Eriko Watanabe, Maekawa A., Kitamura K., Tomita K., Ai Mizuno, Takashi Sato, Mary Ann Suico, Hirofumi Kai
  • Journal Title: Exp. Cell Res. 315 Pages: 3294-300
  • Peer Reviewed
• [Journal Article] A novel combination of mild electrical stimulation and hyperthermia: general concepts and applications (review) (2009)
  • Author(s): Hirofumi Kai, Mary Ann Suico, Saori Morino, Tatsuya Kondo, Mariko Oba, Mikiko Noguchi, Tsuyoshi Shuto, Eiichi Araki
  • Journal Title: Int. J. Hyperthermia 25 Pages: 655-660
  • Peer Reviewed
• [Journal Article] The expression of antimicrobial peptide lysozyme is increased by treatment (2009)
  • Author(s): Mary Ann Suico, Atsushi Tanaka, Tsuyoshi Shuto, Hirofumi Kai
  • Journal Title: J. Health Sci. 55 Pages: 456-462
  • Peer Reviewed
• [Journal Article] The Endoplasmic Reticulum-associated Degradation of Transthyretin Variants Is Negatively Regulated by BiP in Mammalian Cells. (2009)
  • Author(s): Susuki S, et al.
  • Journal Title: J Biol Chem. 284(13) Pages: 8312-8321
  • Peer Reviewed
• [Journal Article] Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury. (2009)
  • Author(s): Oba M, et al.
  • Journal Title: J Surg Res. (in press)
  • Peer Reviewed
• [Journal Article] Calreticulin positively regulates the expression and function of epithelial sodium channel. (2009)
  • Author(s): Sugahara T, et al.
  • Journal Title: Exp Cell Res. 315(19) Pages: 3294-3300
  • Peer Reviewed
• [Journal Article] Role of the glutamic acid 54 residue in transthyretin stability and thyroxine binding. (2009)
  • Author(s): Miyata M, et al.
  • Journal Title: Biochemistry 49(19) Pages: 114-123
  • Peer Reviewed
• [Journal Article] A novel combination of mild electrical stimulation and hyperthermia : general concepts and applications. (2009)
  • Author(s): Kai H, et al.
  • Journal Title: Int J Hyperthermia. 25(8) Pages: 655-660
  • Peer Reviewed
• [Journal Article] and Hirofumi Kai Phosphatidic acid metabolism regulate the intracellular trafficking and retrotranslocation of cystic fibrosis transmembrane conductance regulator (2008)
  • Author(s): Yasuaki Hashimoto, Tsukasa Okiyoneda, Kazutsune Harada, Keiko Ueno, Takuya Sugahara, Atsushi Yamashita, Tsuyoshi Shuto, Mary Ann Suico
  • Journal Title: Biochem. Biochim. Acta (Mol. Cell Res. ) 1783 Pages: 153-162
  • Peer Reviewed
• [Journal Article] Multi-elemental analysis of serum and amyloid fibrils in familial amyloid polyneuropathy patients (2008)
  • Author(s): Susuki S., Ando Y., Sato T., Nishiyama M., Miyata M., Suico MA., Shuto T., Kai H.
  • Journal Title: Amyloid (The Journal of Protein Folding Disorders) 15 Pages: 108-116
  • Peer Reviewed
• [Journal Article] Interferon-gamma down-regulated Hsp27 and enhances hyperthermia-induced tumor cell death in vitro and tumor suppression in vivo (2008)
  • Author(s): Oba M., Yano S., Shuto T., Suico M.A., Eguma A., Kai H.
  • Journal Title: Int. J. Oncol. 32 Pages: 1317-1324
  • Peer Reviewed
• [Journal Article] Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DF508 CFTR. Biochem (2008)
  • Author(s): Okiyoneda T., Niibori A., Harada K., Kohno T., Michalak M., Duszyk M., Wada I., Ikawa M., Shuto T., Suico M. A, Kai H.
  • Journal Title: Biochim. Acta (Mol. Cell Res. ) 1783 Pages: 1585-94
  • Peer Reviewed
• [Journal Article] DNA demethylation-dependent enhancement of toll-like receptor-2 gene expression in cystic fibrosis epithelial cells involves SP1-activated transcription (2008)
  • Author(s): Takashi Furuta, Tsuyoshi Shuto, Shogo Shimasaki, Yuko Ohira, Mary Ann Suico, Dieter C Gruenert, Hirofumi Kai
  • Journal Title: BMC Mol Biol. 9 Pages: 39-39
  • Peer Reviewed
• [Journal Article] p53 regulates TLR3 expression and function in human epithelial cell lines (2008)
  • Author(s): Manabu Taura, Ayaka Eguma, Mary Ann Suico, Tsuyoshi Shuto, Tomoaki Koga, Kensei Komatsu, Takefumi Komune, Takashi Sato, Hideyuki Saya, Jian-Dong Li, Hirofumi Kai
  • Journal Title: Mol. Cell. Biol. 28 Pages: 6557-67
  • Peer Reviewed
• [Journal Article] Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degredation (2008)
  • Author(s): Saori Morino, Mary Ann Suico, Tatsuya Kondo, Erika Sekimoto, Shuichiro Yano. Tomoko Matsuda, Takashi Matsuno, Tsuyoshi Shuto, Eiichi Araki, Hirohumi Kai
  • Journal Title: J Pharmacol Sci. 108 Pages: 222-226
  • Peer Reviewed
• [Journal Article] Mild Electrical Stimulation with heat shock ameliorates insulin resistance via enhanced insulin signaling. Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degradation (2008)
  • Author(s): Saori Morino, Tatsuya Kondo, Kazunari Sasaki, Hironori Adachi, Mary Ann Suico, Erika Sekimoto, Tomoko Matsuda, Tsuyoshi Shuto, Eiichi Araki, Hirofumi Kai.
  • Journal Title: PLoS ONE 3(12)
  • Peer Reviewed
• [Journal Article] Interferon-gamma down-regulated Hsp27 and enhances hyperthermia-induced tumor cell death in vitro and tumor suppression in vivo (2008)
  • Author(s): Oba M, et.al.
  • Journal Title: Int. J. Oncol. 32 Pages: 1317-1324
  • Peer Reviewed
• [Journal Article] p53 regulates TLR3 expression and function in human epithelial cell lines (2008)
  • Author(s): Taura M, et.al.
  • Journal Title: Mol. Cell. Biol. 28(21) Pages: 6557-67
  • Peer Reviewed
• [Journal Article] Epbedrae Herba, a Component of JapaneSe Herbal Medicine Mao-to, Effidently Activates the Replication of Latent Human Immunode ficiency Virus Type 1 (HIV-1) in a Monocytic Cell Line (2008)
  • Author(s): Murakami T, et.al.
  • Journal Title: Biol. Pharm. Bull. 31(12) Pages: 2334-2337
  • Peer Reviewed
• [Journal Article] Mild Electrical Stimulation with heat shock ameliorates insulin resistance via enhanced insulin signaling. Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degreclation (2008)
  • Author(s): Morino S, et.al.
  • Journal Title: PLoS ONE 3(12)
  • Peer Reviewed
• [Journal Article] Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degredation (2008)
  • Author(s): Morino S, et.al.
  • Journal Title: J Pharmacol Sci. 108 Pages: 222-226
  • Peer Reviewed
• [Presentation] Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury (2009)
  • Author(s): スイコメリーアン
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: 札幌市 ガトーキーニグタム札幌市ホテル
• [Presentation] p53 Regulates TLR3 Expression and Function in Human Epthelial Cell Lines (2008)
  • Author(s): A. Eguma
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-13
• [Presentation] p53 is a critical factor for TLR3 expression and TLR3-mediated cytokine induction in epithelial cells (2008)
  • Author(s): Manabu Taura
  • Organizer: BMB2008(第31回日本分子生物学会年会)
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-06
• [Presentation] p53 regulates TLR3 expression in human epithelial cell lines (2008)
  • Author(s): Mary Ann Suico
  • Organizer: Joint International Symposium on Cell Fate Regulation : Deyelopment, Degeneration and Regeneration in the Central Nervous System
  • Place of Presentation: Rochester, NY, USA
  • Year and Date: 2008-07-16
• [Presentation] p53 Regulates TLR3 Expression and Function in Human Epthelial Cell Lines (2008)
  • Author(s): A. Eguma
  • Organizer: The American Society for Cell Biology 48th Annual Meeting
  • Place of Presentation: Moscone Convention Center, San Francisco, USA
• [Remarks]
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• [Remarks]
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