Characterization and functional analyses of Hic-5 deficient mice

• Project/Area Number: 20790076
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Showa University
• Principal Investigator: KANEYAMA Shuri Showa University, 医学部・生化学, 講師 (10338535)
• Co-Investigator(Renkei-kenkyūsha): TAKEDA Naoki 熊本大学, 生命資源研究支援センター生物資源発研究部門技術開発分野, 助教 (90304998)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Hic-5 / ノックアウトマウス / 動脈硬化 / 細胞接着

Research Abstract

Hic-5(hydrogen peroxide-inducible clone-5) is a focal adhesion adaptor protein proposed as a candidate for a mediator of mechanotransduction. In the present study, we generated Hic-5-deficient mice by targeted mutation. Mice lacking Hic-5 are viable and fertile, and show no obvious histological abnormalities including vasculature. However, after wire injury of the femoral artery in Hic-5 deficient mice, histological recovery of arterial media was delayed due to enhanced apoptosis of vascular wall cells, whereas neointima formation was enhanced. Stretch-induced apoptosis was enhanced in cultured vascular smooth muscle cells from Hic-5 deficient mice.

Research Products

• [Journal Article] Hic-5, an adaptor protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo (2008)
  • Author(s): Kim-Kaneyama JR M, Nose K., Wachi N, Sata M, Enomoto S, Fukabori K, Koh K, Shibanuma M, Nose K.
  • Journal Title: Biochem Biophys Res Commun 376(4) Pages: 682-7
  • Peer Reviewed
• [Journal Article] progenitor cells contribute to neointimal formation in nonirradiated chimeric mice (2008)
  • Author(s): Tanaka K, Sata M, Natori T, Kim-Kaneyama JR, Nose K, Shibanuma M, Hirata Y, Nagai R.
  • Journal Title: FASEB J. 22(2) Pages: 428-36
  • Peer Reviewed
• [Journal Article] Hic-5, an adaptor protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo (2008)
  • Author(s): Kim-Kaneyama JR, et.al.
  • Journal Title: Biochem Biophys Res Commun 376(4) Pages: 682-7
  • Peer Reviewed
• [Presentation] Hic-5, an adaptor protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo (2009)
  • Author(s): 金山朱里
  • Organizer: 日本動脈硬化学会
  • Place of Presentation: 下関
  • Year and Date: 2009-07-17
• [Presentation] ヒトマクロファージからのコレステロール搬出に対するレプチンの抑制作用 (2009)
  • Author(s): 本郷茂樹、渡部琢也、有田茂子、金山朱里、宮崎章
  • Organizer: 日本脂質生化学会
  • Place of Presentation: 名古屋
• [Presentation] Hic-5, an adaptor protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo. (2009)
  • Author(s): 金山朱里
  • Organizer: 日本動脈硬化学会
  • Place of Presentation: 山口県下関市
• [Presentation] 細胞接着斑アダプター分子Hic-5のノックアウトマウス作製と解析 (2008)
  • Author(s): 金山朱里
  • Organizer: 日本分子生物学会年会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12