Elucidation of physiological role of Laeverin, a novel aminopeptidase.

• Project/Area Number: 20790088
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Kansai Medical University
• Principal Investigator: MARUYAMA Masato Kansai Medical University, 医学部, 助教 (00399445)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Laeverin / 絨毛外栄養膜細胞 / エキソペプチダーゼモチーフ / M1アミノペプチダーゼ / 胎盤形成

Research Abstract

Here, we evaluated the roles of His^<379>, comprising the exopeptidase motif, in the enzymatic properties of human Laeverin. Our results indicate that His^<379> plays essential roles in its distinctive enzymatic properties and contributes to maintaining the appropriate structure of the catalytic cavity of the enzyme.

Research Products

• [Journal Article] Histidine 379 of Human Laeverin/AminopeptidaseQ, a Nonconserved Residue within the Exopeptidase Motif, Defines Its Distinctive Enzymatic Properties (2009)
  • Author(s): Masato Maruyama, Naomi Arisaka, Yoshikuni Goto, Yosuke Ohsawa, Hideshi Inoue, Hiroshi Fujiwara, Akira Hattori, Masafumi Tsujimoto
  • Journal Title: The Journal of Biological Chemistry vol.284 Pages: 34692-34702
  • Peer Reviewed
• [Journal Article] Histidine 379 of Human Laeverin/AminopeptidaseQ, a Nonconserved Residue within the Exopeptidase Motif, Defines Its Distinctive Enzymatic Properties (2009)
  • Author(s): Masato Maruyama
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 34692-34702
  • Peer Reviewed
• [Journal Article] In situ hybridization study of the distribution of choline acetyltransferase mRNA and its splice variants in the mouse brain and spinal cord. (2009)
  • Author(s): Stefan Trifonov
  • Journal Title: Neuroscience 159 Pages: 344-357
  • Peer Reviewed
• [Presentation] HISTIDINE-379 OF HUMAN LAEVERIN/AMINOPEPTIDASE Q, A NON-CONSERVED RESIDUE WITHIN THE EXOPEPTIDASE MOTIF, DEFINES ITS DISTINCTIVE ENZYMATIC PROPERTIES (2009)
  • Author(s): Akira Hattori
  • Organizer: 6th General Meeting of the International Proteolysis Society
  • Place of Presentation: Surfers Paradise, QLD, Australia
  • Year and Date: 2009-10-27
• [Presentation] Histidine-379 of human laeverin/aminopeptidase Q, a non-conserved residue within the exopeptidase motif, defines its distinctive enzymatic properties (2009)
  • Author(s): Hattori A, Maruyama M, Arisaka N, Goto Y, Osawa Y, Inoue H, Fujiwara H, Tsujimoto M
  • Organizer: 6th General Meeting of the International Proteolysis Society
  • Place of Presentation: Surfers Paradise, Australia
• [Presentation] ヒトLaeverinの酵素学的性状の発現におけるBis379残基の役割 (2008)
  • Author(s): 有坂尚美
  • Organizer: 病態と治療におけるプロテアーゼとインヒビター学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-08-22
• [Presentation] 点変異導入によるヒトLaeverinのHis379残基の機能解析 (2008)
  • Author(s): 有坂尚美, 丸山正人, 服部明, 辻本雅文
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://www3.kmu.ac.jp/anat2/