The function and regulation of ABCA1 in neuron

• Project/Area Number: 20790091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: OKUHIRA Keiichiro National Institute of Health Sciences, 機能生化学部, 主任研究官 (10425671)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脂質 / 神経

Research Abstract

In this study, we investigated the role of ATP binding cassette transporter A1 (ABCA1) in the neuronal growth and collapse of mouse neuroblastoma or primary rat neuron. In rat dorsal root ganglion (DRG) neurons, there was no clear difference in the neuronal growth between control and ABCA1-silencing cells. However, the repair of the damaged neuron was retarded by suppressing the ABCA1 expression, indicating that ABCA1 may function for the restoration of neuron.

Research Products

• [Journal Article] (*corresponding author) Binding of PDZ-RhoGEF to ATP-Binding Cassette Transporter A1 (ABCA1) induces cholesterol efflux through RhoA activation and prevention of transporter degradation. (2010)
  • Author(s): Okuhira K*, Fitzgerald ML, Tamehiro N, Ohoka N, Suzuki K, Sawada J, Naito M, Nishimaki-Mogami T.
  • Journal Title: J Biol Chem (in press)
• [Journal Article] SPTLC1 binds ABCA1 to negatively regulate trafficking and cholesterol efflux activity of the transporter. (2008)
  • Author(s): Tamehiro N, Zhou S, Okuhira K, Benita Y, Brown CE, Zhuang DZ, Latz E, Hornemann T, von Eckardstein A, Xavier RJ, Freeman MW, Fitzgerald ML.
  • Journal Title: Biochemistry 47(23) Pages: 6138-47
• [Journal Article] The RXR agonists PA024 and HX630 have different abilities to activate LXR/RXR and to induce ABCA1 expression in macrophage cell lines. (2008)
  • Author(s): Nishimaki-Mogami T, Tamehiro N, Sato Y, Okuhira K, Sai K, Kagechika H, Shudo K, Abe-Dohmae S, Yokoyama S, Ohno Y, Inoue K, Sawada J.
  • Journal Title: Biochem Pharmacol 76(8) Pages: 1006-13
• [Journal Article] SPTLC1 binds ABCA1 to negatively regulate trafficking and cholesterol efflux activity of the transporter (2008)
  • Author(s): Tamehiro, et al.
  • Journal Title: Biochemistry 47(23) Pages: 6138-47
  • Peer Reviewed
• [Journal Article] The RXR agonists PA024 and HX630 have different abilities to activate LXR/RXR and to induce ABCA1 expressin in macrophage cell lines (2008)
  • Author(s): Nishimaki-Mogami T, et al.
  • Journal Title: Biochem Pharmacol. 76(8) Pages: 1006-13
  • Peer Reviewed
• [Journal Article] Binding of PDZ-RhoGEF to ATP-binding cassette transporter Al (ABCA1) induces cholesterol efflux through RhoA activation and prevention of transporter degradation
  • Author(s): Okuhira, et al.
  • Journal Title: The Journal of Biological Chemistry (印刷中)
  • Peer Reviewed
• [Presentation] グアニンヌクレオチド交換因子によるABCA1活性制御機構 (2010)
  • Author(s): 奥平桂一郎
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-30
• [Presentation] 最上(西巻)知子グアニンヌクレオチド交換因子によるABCA1活性制御機構 (2010)
  • Author(s): 奥平桂一郎, 大岡伸通, 崔紅艶, 岩崎香里, 内藤幹彦
  • Organizer: 日本薬学会130年会
• [Presentation] ABCA1相互作用タンパク質とRhoAによるHDL形成制御機構 (2009)
  • Author(s): 奥平桂一郎
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-31
• [Presentation] ABCA1相互作用タンパク質とPHoAによるHDL形成制御機構 (2009)
  • Author(s): 奥平桂一郎
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] 最上(西巻)知子ABCA1相互作用タンパク質とRhoAによるHDL形成制御機構 (2009)
  • Author(s): 奥平桂一郎, 大岡伸通, 崔紅艶, 澤田純一
  • Organizer: 日本薬学会129年会
• [Presentation] 最上(西巻)知子ABCA1相互作用タンパク質とRhoAによるHDL形成制御機構 (2009)
  • Author(s): 奥平桂一郎, 大岡伸通, 崔紅艶, 内藤幹彦
  • Organizer: 第51回日本脂質生化学会