Development of transcriptional Zn(II) sensor based on protein conformational change
| Project/Area Number |
20790099
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
HORI Yuichiro Osaka University, 工学研究科, 助教 (00444563)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 構造変化 / 亜鉛イオン / 亜鉛フィンガー / ホメオドメイン / 転写因子 / センサー / DNA結合 / 人工蛋白質 / 構造変換 / ヘリックス・ターン・ヘリックス |
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| Research Abstract |
DNA-binding proteins have been attracting attention in the field of medical and life sciences and applied to the targeting and transcriptional control of particular genes. In this project, a Zn(II)-sensor protein, which binds to DNA, is designed by utilizing Antennafinger (Ant-F). Ant-F is an artificial protein, which induce structural change by binding Zn(II). This research will provide a basic tool, which could lead to the development of the technique for the detection of Zn(II) by transcriptional activity.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article]2010
Author(s)
Hori, Y., Egashira, Y., Kamiura, R., Kikuchi, K.
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Journal Title
Chembiochem 11
Pages: 646-648
Related Report
Peer Reviewed
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