Mechanisms of streptococcal infection investigated by whole genomic tiling array

• Project/Area Number: 20790111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: MARUYAMA Fumito The University of Tokyo, 大学院・生命理工学研究科, 助教 (30423122)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 微生物・感染症学 / タイリングアレイ / トランスクリプトーム / non-coding RNA / A群レンサ球菌 / non- codingRNA / レンサ球菌

Research Abstract

Genomic contents of group A streptococci is rich in A or T nucleotides. This causes problem in determination of transcription factors and/or prediction of open reading regions. Firstly, we have established the method to analyze the transcriptome of streptococcal cells inside host eukaryotic cells with cDNA microarray and tiling array. The data obtained from the arrays revealed the existence of 58 novel non-coding RNA and importance of unknown genes to combat with host immune system. These things suggests various pathogenicities of group A streptococci are resulted from these non-coding RNA and hypothetical genes.

Research Products

• [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content (2009)
  • Author(s): F. Maruyama, M. Kobata, K. Kurokawa, K. Nishida, A. Sakurai, K. Nakano, R. Nomura, S. Kawabata, T. Ooshima, K. Nakai, M. Hattori, S. Hamada, I. Nakagawa
  • Journal Title: BMC Genomics 10 Pages: 358-358
  • Peer Reviewed
• [Journal Article] Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids. (2009)
  • Author(s): F. Maruyama*, T. Nozawa*, C. Aikawa, A. Sakurai, I. Nakagawa
  • Journal Title: ournal of Bioscience and Bioengineering 104 Pages: 471-473
  • NAID: 110007225791
  • Peer Reviewed
• [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content (2009)
  • Author(s): F.Maruyama, M.Kobata, K.Kurokawa, K.Nishida, A.Sakurai, K.Nakano, R.Nomura, S.Kawabata, T.Ooshima, K.Nakai, M.Hattori, S.Hamada, I.Nakagawa
  • Journal Title: BMC Genomics 10 Pages: 358-358
  • Peer Reviewed
• [Journal Article] Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids (2009)
  • Author(s): F.Maruyama, T.Nozawa, C.Aikawa, A.Sakurai, I.Nakagawa
  • Journal Title: Journal of Bioscience and Bioengineering 104 Pages: 471-473
  • NAID: 110007225791
  • Peer Reviewed
• [Journal Article] Cost- effective DNA sequencing and Template Preparation from Bacterial Colony and Plasmid. (2009)
  • Author(s): F. Maruyama
  • Journal Title: J. Biosci. Bioeng. in press
  • NAID: 110007225791
  • Peer Reviewed
• [Journal Article] Application of real- time long and short polymerase chain reaction for sensitive monitorinE of the fate of extracellular nlasmid DNA. (2008)
  • Author(s): F. Maruyama
  • Journal Title: Microb. Environ. 233 Pages: 229-236
  • Peer Reviewed
• [Journal Article] Streptococcus pyogenes degrades extracellular matrix in chondrocytes via MMP- 1 (2008)
  • Author(s): A. Sakurai
  • Journal Title: Biochem. Biophys. Res. Commun 373 Pages: 450-454
  • Peer Reviewed
• [Journal Article] Current and future biotechnological applications of bacterial phytases and phvtase- producine bacteria. (2008)
  • Author(s): M. Jorquera
  • Journal Title: Microb. Environ. 23 Pages: 182-191
  • Peer Reviewed
• [Journal Article] Reactive oxygen species induced by fibronectin-mediated Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells.
  • Author(s): C. Aikawa, T. Nozawa, F. Maruyama, K. Tsumoto, S. Hamada, I. Nakagawa
  • Journal Title: Cellular Microbiology (in press)
  • Peer Reviewed
• [Journal Article] Reactive oxygen species induced by fibronectin-mediated Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells
  • Author(s): C.Aikawa, T.Nozawa, F.Maruama, K.Tsumoto, S.Hamada, I.Nakagawa
  • Journal Title: Cellular Microbiology (In press)
  • Peer Reviewed
• [Presentation] レンサ球菌属ゲノム情報に基づく種特異的進化機構の解析 (2010)
  • Author(s): 丸山史人
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] ビブリオ科細菌における薬剤耐性プラスミド全塩基配列解析とその伝達機構 (2009)
  • Author(s): 野中里佐・丸山史人・宮本学・黒川顕・増田道明
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-21
• [Presentation] 次世代シーケンサーの基礎と細菌学への応用 (2009)
  • Author(s): 丸山史人
  • Organizer: 第3回細菌学・若手コロセウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-26
• [Presentation] 芳香族化合物による土壌撹乱に対するバクテリア群集の環境応答のメタゲノム解析 (2009)
  • Author(s): 森宙史, 加藤広海, 堂園亜由美, 大坪嘉行, 宮腰昌利, 丸山史人, 豊田敦, 藤山秋佐夫, 永田裕二, 黒川顕, 津田雅孝
  • Organizer: 細菌学若手コロッセウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-26
• [Presentation] Species-specific evolutionary strategies revealed from genome and transcriptome of Streptococcus pyogenes and S. mutans (2009)
  • Author(s): F. Maruyama, H. Mori, C. Aikawa, T. Nozawa, K. Kurokawa, I. Nakagawa
  • Organizer: ProkaGENOMICS 2009
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2009-10-04
• [Presentation] A visualization tool for comparative community analyses using nearest relatives (2009)
  • Author(s): H. Mori, F. Maruyama, K. Kurokawa
  • Organizer: ProkaGENOMICS 2009
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2009-10-04
• [Presentation] Species-specific evolutionary strategies revealed from genome and transcriptome of Streptococcus pyogenes and S.mutans (2009)
  • Author(s): F.Maruyama, H.Mori, C.Aikawa, T.Nozawa, K.Kurokawa, I.Nakagawa
  • Organizer: ProkaGENOMICS 2009
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2009-10-04
• [Presentation] 口腔領域レンサ球菌による宿主上皮細胞への付着侵入と炎症の誘導 (2009)
  • Author(s): 桜井敦朗, 岡橋暢夫, 中田匡宣, 寺尾豊, 丸山史人, 川端重忠, 大嶋隆
  • Organizer: 第18回Lancefieldレンサ球菌研究会
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-26
• [Presentation] 口腔領域レンサ球菌による宿主上皮細胞への付着侵入と炎症の誘導 (2009)
  • Author(s): 桜井敦朗, 岡橋暢夫, 中田匡宣, 寺尾豊, 丸山史人, 川端重忠, 大嶋隆
  • Organizer: 第18回 Lancefield レンサ球菌研究会
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-26
• [Presentation] Streptococcus mutansおよびS. pyogenesゲノム・トランスクリプトームからみた種分化機構の解明 (2009)
  • Author(s): 丸山史人, 大野雅幸, 相川知広, 野澤孝志, 桜井敦朗, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] A群レンサ球菌の全ゲノム発現解析に基づく細胞内生存機構の解明 (2009)
  • Author(s): 大野雅幸, 丸山史人, 相川知広, 野澤孝志, 桜井敦朗, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] オートファジー誘導によるA群レンサ球菌感染上皮の細胞死制御機構の解析 (2009)
  • Author(s): 相川知広, 野澤孝志, 大野雅幸, 丸山史人, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] A群レンサ球菌感染時にオートファジーを誘導するNLRファミリーと相互作用する宿主因子の機能解析 (2009)
  • Author(s): 野澤孝志, 桜井敦朗, 大野雅幸, 相川知広, 丸山史人, 中川一路
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] Streptococuss mutansおよびS.pyogenesゲノム・トラノスクリプトームからみた種分化機構の解明 (2009)
  • Author(s): 丸山史人
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] Streptococcus mutansおよびS. pyogenesゲノム・トランスクリプトームからみた種分化機構の解明 (2009)
  • Author(s): 丸山史人, 大野雅幸, 相川知広, 野澤孝志, 桜井敦朗, 中川一路
  • Organizer: ゲノム微生物学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] Streptococcus muansおよびS.pyogenesゲノム・トランーヌクリプトームからみた種分化機構の解明 (2009)
  • Author(s): 丸山史人
  • Organizer: ゲノム微生物学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] Group A streptococcus transcriptome aynamics insiae numan cell reveals bacterial adaptation and survival against innate immune systems (2008)
  • Author(s): M. Ono
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Hyogo
  • Year and Date: 2008-09-08
• [Presentation] Ilaentirication or minimum structure or patnogen- associatea molecular pattern and the recognition mechanism'in autophagy induction against group (2008)
  • Author(s): T. Nozawa
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Hyogo
  • Year and Date: 2008-09-08
• [Presentation] 比較ゲノム・トランスクリトームにつくStreptococcus mutansおよびS.pyogenesの種レベルでの進化機構の解明 (2008)
  • Author(s): 丸山史人
  • Organizer: 特定領域研究「ゲノム」4領域合同班会議
  • Place of Presentation: 兵庫
  • Year and Date: 2008-08-25
• [Presentation] 比ゲノム・トランスクリトームにつくStreptococcus mutansおよびS.pyogenesゲノム進化機能の解析 (2008)
  • Author(s): 丸山史人
  • Organizer: 第2回細菌学・若手コロッセウム
  • Place of Presentation: 神奈川
  • Year and Date: 2008-08-03
• [Book] 難培養微生物研究の最新技術II-ゲノム解析を中心とした最前線と将来展望-Current Technology and Perspectives for Yet-uncultivated Microbial Resources (2010)
  • Author(s): 森宙史、丸山史人、黒川顕
  • Publisher: シーエムシー出版(未定(印刷中))