Investigation of peptidoglycan modification and its role in virulence of Salmonella enterica serovar Typhimurium.

• Project/Area Number: 20790119
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: Doshisha Women's College of Liberal Arts
• Principal Investigator: KAWANO Miyuki Doshisha Women's College of Liberal Arts, 薬学部, 特別任用助教 (10454498)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 微生物・感染症学 / マクロファージ / エンドトキシン / ペプチドグリカン / グラム陰性菌外膜 / グラム陰性菌 / 外膜修飾 / ペプチドグリカン修飾 / リゾチーム

Research Abstract

Salmonella Typhimurium LpxR potentially detoxifies lipid A by 3'-0-deacylation. The intracellular growth of the lpxR-null strain was lower than that of the wild-type strain, and the expression level of iNOS was enhanced in the cells infected with the lpxR-null strain. We also identified the candidate genes for peptidoglycan N-deacetylase in S.Typhimurium. The deletion of the gene also affected the intracellular growth. These results suggested that the cell wall modifications were important for the intracellular growth of S.Typhimurium within host cells.

Research Products

• [Journal Article] Mutations in the lipid A deacylase PagL which release the enzyme from its latency affect the ability of PagL to interact with lipopolysaccharide in Salmonella enterica serovar Typhimurium. (2010)
  • Author(s): 眞鍋貴行, 河野美幸, 川崎清史
  • Journal Title: Biochem Biophys Res Commun. (投稿中)
  • Peer Reviewed
• [Journal Article] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages. (2010)
  • Author(s): 河野美幸, 眞鍋貴行, 川崎清史
  • Journal Title: FEBS Letters vol.584 Pages: 207-212
  • Peer Reviewed
• [Journal Article] Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages (2010)
  • Author(s): 河野美幸
  • Journal Title: FEBS letters 584 Pages: 207-212
  • Peer Reviewed
• [Presentation] LpxRによるリピドA脱アシル化はサルモネラの効率的な細胞内増殖に必要である (2009)
  • Author(s): 河野美幸, 眞鍋貴行, 川崎清史
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] サルモネラLPS脱アシル化酵素PagLの活性調節に関与するアミノ酸残基は他の外膜タンパク質との相互作用に重要である (2009)
  • Author(s): 眞鍋貴行, 河野美幸, 川崎清史
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Lack of a lipid A modification affects intracellular growth of Salmonella Typhimurium in RAW264.7 cells (2009)
  • Author(s): 河野美幸
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: ポートアイランド(神戸)
  • Year and Date: 2009-10-24
• [Presentation] Salmonella typhimurium mutant lacking a lipid A modification exhibited reduced intracellular growth in RAW264 (2008)
  • Author(s): 河野美幸, 川崎清史
  • Organizer: 7 cells第81回日本生化学会大会・第31回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Salmonella typhimurium mutant lacking a lipid A modification exhibited reduced intracellular growth in RAW264.7 cells (2008)
  • Author(s): 河野美幸
  • Organizer: 第81回日本生化学会大会・第31回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12