| Project/Area Number |
20790122
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Environmental pharmacy
|
|---|
| Research Institution | Kobe Pharmaceutical University |
|---|
Principal Investigator |
YAMAKI Kouya Kobe Pharmaceutical University, 薬学部, 講師 (00351768)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | ETBE / バイオエタノール / 免疫 / T細胞 |
|---|
| Research Abstract |
To determine the effect of ETBE, a bioethanol-derived fuel oxygenate, on immune response, the adjuvant effect of ETBE was investigated. First, we injected ovalbumin and 0.1 to 3 % ETBE in saline solution to mice. After 21 days, anti-OVA IgG levels in serum of the mice were measured. The mice injected with 3 % ETBE were dead within a day. ETBE at concentrations between 0.1 to 1 % did not affect anti-OVA IgG level, thus single injection of ETBE with the antigen was considered to have no adjuvant effect on the antibody production. Next, we injected 1 % ETBE 5 times per week from day 0 to day 20 to mice immunized with ovalbumin on day 0. The levels of anti-OVA IgG in serum of immunized mice with successive injection of 1 % ETBE were higher than those of immunized mice without injection of ETBE. This result indicated that the chronic exposure of ETBE might exert adjuvant activity.
In addition, 1 % ETBE treatment attenuated the degranulation of mast cell line RBL2H3.
These results indicated 1 % ETBE may potentially affect immune responses. Although low concentrations of ETBE evaporated from fuels might not affect human health, accidental exposure to a high concentration of ETBE is likely to affect immune responses.
|
