Developmental research for measurement of acrolein and polyamine oxidase as markers for diagnosis of brain infarction

• Project/Area Number: 20790143
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Chiba Institute of Science
• Principal Investigator: TOMITORI Hideyuki Chiba Institute of Science, 薬学部, 講師 (30337381)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 生理活性物質 / 脳・神経 / バイオマーカー / 臨床化学 / 薬物治療・トキシコロジー / ポリアミン / アクロレイン / ポリアミンオキシダーゼ / 脳梗塞

Research Abstract

Polyamines play important roles in cell proliferation and differentiation. However, acrolein that is highly toxic compound is produced by polyamine oxidation. In this study, new methods for measuring acrolein and polyamine oxidases, and molecular mechanism of acrolein toxicity on cell growth have been studied. New findings in this study have been described below.

Research Products

• [Journal Article] Identification of acrolein-conjugated protein in plasma of patients with brain infarction. Biochem (2010)
  • Author(s): Yoshida, M., Higashi, K., Jin, L., Machi, Y., Suzuki, T., Masuda, A., Dohmae, N., Suganami, A., Tamura, Y., Nishimura, K., Toida, T., Tomitori, H., Kashiwagi, K., Igarashi, K.
  • Journal Title: Biophys. Res. Commun. 391 Pages: 1234-1239
• [Journal Article] Identification of acrolein-conjugated protein in plasma of patients with brain infarction. (2010)
  • Author(s): Yoshida, M.
  • Journal Title: Biochem.Biophys.Res.Commun. 391 Pages: 1234-1239
  • Peer Reviewed
• [Journal Article] Enhancement of the synthesis of RpoE and StpA by polyamines at the level of translation in Escherichia coli under heat shock conditions (2009)
  • Author(s): Terui, Y., Higashi, K., Tabei, Y., Tomitori, H., Yamamoto, K., Ishihama, A., Igarashi, K., Kashiwagi, K.
  • Journal Title: J. Bacteriol 191 Pages: 5348-5357
• [Journal Article] Acrolein, IL-6 and CRP as markers of silent brain infarction (2009)
  • Author(s): Yoshida, M., Tomitori, H., Machi, Y., Katagiri, D., Ueda, S., Horiguchi, K., Kobayashi, E., Saeki, N., Nishimura, K., Ishii, I., Kashiwagi, K., Igarashi, K.
  • Journal Title: Atherosclerosis 203 Pages: 557-562
• [Journal Article] Acrolein toxicity: Comparison with reactive oxygen species (2009)
  • Author(s): Yoshida, M., Tomitori, H., Machi, Y., Hagihara, M., Higashi, K., Goda, H., Ohya, T., Niitsu, M., Kashiwagi, K., Igarashi, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 313-318
• [Journal Article] Acrolein, IL-6 and CRP as markers of silent brain infarction (2009)
  • Author(s): Yoshida, M.
  • Journal Title: Atherosclerosis 203 Pages: 557-562
  • Peer Reviewed
• [Journal Article] Enhancement of the synthesis of RpoE and StpA by polyamines at the level of translation in Escherichia coli under heat shock conditions (2009)
  • Author(s): Terui, Y.
  • Journal Title: J.Bacteriol. 191 Pages: 5348-5357
  • Peer Reviewed
• [Journal Article] Acrolein toxicity : Comparison with reactive oxygen species (2009)
  • Author(s): Yoshida, M., et.al.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 313-318
  • Peer Reviewed
• [Journal Article] Binding of spermine and ifenprodil to a purified, soluble regulatory domain of the N-methyl-D-aspartate receptor (2008)
  • Author(s): Han, X., Tomitori, H., Mizuno, S., Higashi, K., Full, C., Fukiwake, T., Terui, Y., Leewanich, P., Nishimura, K., Toida, T., Williams, K., Kashiwagi, K., Igarashi, K.
  • Journal Title: J. Neurochem 107 Pages: 1566-1577
• [Journal Article] Binding of spermine and ifenprodil to a purified, soluble regulatory domain of the N-methyl-D-aspartate receptor (2008)
  • Author(s): Han, X., et.al.
  • Journal Title: J. Neurochem. 107 Pages: 1500-1577
  • Peer Reviewed
• [Presentation] アダマンタンダイマーの合成と細胞増殖への影響 (2010)
  • Author(s): 荒野直紀
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山コンベンションセンター、岡山
  • Year and Date: 2010-03-29
• [Presentation] アダマンタンダイマーの合成と細胞増殖への影響 (2010)
  • Author(s): 荒野直紀
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山コンベンションセンター岡山
  • Year and Date: 2010-03-29
• [Presentation] ポリアミンにより合成促進されるribosome modulation factorの大腸菌生存率維持に果たす役割 (2010)
  • Author(s): 秋山真律子
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山コンベンションセンター、岡山
  • Year and Date: 2010-03-28
• [Presentation] ポリアミンにより合成促進されるribosome modulation factorの大腸菌生存率維持に果たす役割 (2010)
  • Author(s): 秋山真律子
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山コンベンションセンター岡山
  • Year and Date: 2010-03-28
• [Presentation] アクロレイン耐性細胞の樹立とそのアクロレイン毒性除去機構 (2009)
  • Author(s): 中村瑞穂
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2009-10-23
• [Presentation] ポリアミン酸化により産生されるアクロレインの毒性:活性酸素種との比較 (2009)
  • Author(s): 富取秀行
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2009-10-23
• [Presentation] Isolation and characterization of an acrolein tolerant mutant of FM3A cells (2009)
  • Author(s): 富取秀行
  • Organizer: 2009 GORDON RESEARCH CONFERENCE ON POLYAMINES
  • Place of Presentation: Waterville Valley, New Hampshire, USA
  • Year and Date: 2009-06-24
• [Presentation] Polyamine effect on protein synthesis and its mechanism (2009)
  • Author(s): Kashiwagi, K., Terui, Y., Higashi, K., Okudaira, H., Ochiai, E., Tomitori, H., Nishimura, K., Yamamoto, K., Ishihama, A., Igarashi, K.
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
  • Year and Date: 2009-06-22
• [Presentation] スペルミン及びイフェンプロジルのNMDA受容体調節領域への結合能 (2009)
  • Author(s): 韓霞
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] アクロレイン耐性細胞の樹立とその性質の検討 (2009)
  • Author(s): 富取秀行
  • Organizer: 日本ポリアミン研究会 第23回研究発表会
  • Place of Presentation: 藍野大学、大阪
  • Year and Date: 2009-01-23
• [Presentation] アクロレイン耐性細胞の樹立とその性質の検討 (2009)
  • Author(s): 富取秀行
  • Organizer: 日本ポリアミン研究会第23回研究発表会
  • Place of Presentation: 藍野大学
  • Year and Date: 2009-01-23
• [Presentation] PREDICTION OF SILENT BRAIN INFARCTION BY ACROLEIN, POLYAMINE OXIDASE (2008)
  • Author(s): 富取秀行
  • Organizer: IL-6 AND CRP Polyamines: Forty years of Mammalian Ornithine Decarboxylase
  • Place of Presentation: Kuopio, Finland
  • Year and Date: 2008-06-28
• [Presentation] PREDICTION OF SILENT BRAIN INFARCTION BY ACROLEIN POLYAMINE OXIDASE, IL-6 AND CRP (2008)
  • Author(s): 富取秀行
  • Organizer: Polyamines : Forty years of Mammalian Ornithine Decarboxylase
  • Place of Presentation: Kuopio, Finland
  • Year and Date: 2008-06-28