Study of side effect reduction of anticancer agents by regulation of exogenous gene expression

• Project/Area Number: 20790152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Himeji Dokkyo University
• Principal Investigator: KINOSHITA Atsushi Himeji Dokkyo University, 薬学部, 講師 (60454766)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 医療薬剤学 / 遺伝子治療 / 抗悪性腫瘍薬 / 遺伝子発現制御 / CMVプロモーター / フリーラジカルスカベンジャー / 活性酸素種 / MAPK / 抗悪件腫瘍薬

Research Abstract

For the purpose of regulation of transgene expression after gene introduction in gene therapy, the levels of CMV-promoter-driven transgene expression was analyzed when transgene introduced cells was exposed by anticancer agents. The levels of CMV-promoter-driven transgene expression were increased by doxorubicin (Dox) exposure. The induction of CMV-promoter-driven transgene expression by Dox was decreased by co-treatment of edaravone. We also found that phosphorylation pathway of AP-1 having CMV-promoter binding ability was activated by Dox.

Research Products

• [Journal Article] Effects of anticancer agents and scavengers for CMV-promoter-driven exogenous gene expression in genetically modified cells (2009)
  • Author(s): A.Kinoshita, D.Kobayashi, Y.Saitoh, F.Komada
  • Journal Title: Journal of Pharmacy and Pharmacology Vol.61, No.4 Pages: 527-531
  • Peer Reviewed
• [Journal Article] Effects of anticancer agents and scavengers on CMV-promoter-driven exogenous gene expression in genetically modified cells (2009)
  • Author(s): Atsushi Kinoshita
  • Journal Title: Journal of Pharmacy and Pharmacology Vol.61 Pages: 527-531
  • Peer Reviewed
• [Journal Article] Regulation of CMV promoter-driven exogenous gene expression with doxorubicin in genetically modified cells (2008)
  • Author(s): A.Kinoshita, D.Kobayashi, Y.Hibino, T.Isago, K.Uchino, K.Yagi, M.Hirai, Y.Saitoh, F.Komada
  • Journal Title: Journal of Pharmacy and Pharmacology Vol.60, No.12 Pages: 1659-1665
  • Peer Reviewed
• [Journal Article] Regulation of CMV promoter-driven exogenous gene expression with doxorubicin in genetically modified cells (2008)
  • Author(s): Atsushi Kinoshita
  • Journal Title: Journal of Pharmacy and Pharmacology 60 Pages: 1659-1665
  • Peer Reviewed
• [Presentation] CMVプロモーター制御下の遺伝子発現におよぼす抗悪性腫瘍薬の効果とAP-1リン酸化経路の解析 (2010)
  • Author(s): 木下淳
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] CMVプロモーター制御下の遺伝子発現におよぼす抗悪性腫瘍薬の効果とAP-1リン酸化経路の解析 (2010)
  • Author(s): 木下淳
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ
  • Year and Date: 2010-03-28
• [Presentation] 抗悪性腫瘍薬による副作用の軽減を目的とした新規遺伝子治療の構築 (2009)
  • Author(s): 木下淳
  • Organizer: 電気学会光・量子デバイス研究会
  • Place of Presentation: 神戸
  • Year and Date: 2009-05-08
• [Presentation] 抗悪性腫瘍薬による副作用の軽減を目的とした新規遺伝子治療の構築 (2009)
  • Author(s): 木下淳
  • Organizer: 電気学会 光・量子デバイス研究会
  • Place of Presentation: 神戸大学 六甲台キャンパス
  • Year and Date: 2009-05-08
• [Presentation] CMVプロモーター制御下の遺伝子発現におよぼす抗悪性腫瘍薬およびscavengerの効果 (2009)
  • Author(s): 木下淳
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] CMVプロモーター制御下の遺伝子発現におよぼす抗悪性腫瘍薬およびscavengerの効果 (2009)
  • Author(s): 木下淳
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26