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Elucidation of molecular mechanisms of gliogenesis and an attempt to induce neurogenesis in the adult spinal cord

Research Project

Project/Area Number 20790161
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTohoku University

Principal Investigator

KITADA Masaaki  Tohoku University, 大学院・医学系研究科, 准教授 (80324614)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords細胞分化 / 神経幹細胞 / 神経前駆細胞 / 成体脊髄上衣細胞 / Notchシグナリンググリア細胞新生 / 神経細胞新生 / 脊髄上衣細胞 / Notchシグナリング / グリア細胞発生 / 脊髄
Research Abstract

We have found the activity of the ependymal cells as glial precursor cells in the adult spinal cord. In this study, we elucidated that proliferation and differentiation of the ependymal cells are regulated by the intracellular signaling of Notch pathway. Also, we explored the possibility to convert this gliogenesis into neurogenesis and thus identified the factor which can regulate gliogenesis and neurogenesis.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (29 results)

All 2010 2009 2008

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (14 results)

  • [Journal Article] Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration.2010

    • Author(s)
      Wakao S*, Hayashi T*, Kitada M*, et al (+11 people)
    • Journal Title

      Exp Neurol 223(2)

      Pages: 537-547

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unique multipotent stem cells in adult human mesenchymal cell populations.2010

    • Author(s)
      Kuroda Y*, Kitada M*, et al (+14 people)
    • Journal Title

      Proc Natl Acad Sci U S A 107(19)

      Pages: 8639-8643

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration2010

    • Author(s)
      Wakao S, Hayashi T, Kitada M
    • Journal Title

      Exp Neurol (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique multipotent cells in adult human mesenchymal cell populations2010

    • Author(s)
      Kuroda Y, Kitada M
    • Journal Title

      Proc Natl Acad Sci USA (印刑中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction system of neural and muscle lineage cells from bone marrow stromal cells: a new strategy for tissue reconstruction in degenerative diseases.2009

    • Author(s)
      Kitada M, Dezawa M.
    • Journal Title

      Histol Histopathol 24(5)

      Pages: 631-642

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new monoclonal antibody, A3B10, specific for astrocyte-lineage cells recognizes calmodulin-regulated spectrin-associated protein 1 (Camsap1).2009

    • Author(s)
      Yamamoto M, Yoshimura K, Kitada M, et al (+3 people)
    • Journal Title

      J Neurosci Res 87(2)(Review)

      Pages: 503-513

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Practical induction system for dopamine-producing cells from bone marrow stromal cells using spermine-pullulan- mediated reverse transfection method.2009

    • Author(s)
      Nagane K, Kitada M, et al (+3 people)
    • Journal Title

      Tissue Eng Part A 15(7)

      Pages: 1655-1665

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Committed neural progenitor cells derived from genetically modified bone marrow stromal cells ameliorate deficits in a rat model of stroke.2009

    • Author(s)
      Hayase M, Kitada M, et al (+6 people)
    • Journal Title

      J Cereb Blood Flow Metab 29(8)

      Pages: 1409-1420

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction system of neural and muscle lineage cells from bone marrow stromal cells : a new strategy for tissue reconstruction in degenerative diseases2009

    • Author(s)
      Kitada M
    • Journal Title

      Histol Histopathol 24

      Pages: 631-642

    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Practical induction system dopamine-producing cells from bone marrow stromal cells using spermine-pullulan-mediated reverse transfection method2009

    • Author(s)
      Nagane K, Kitada M
    • Journal Title

      Tissue Eng Part A 15

      Pages: 1655-1665

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Committed neural progenitor cells derived from genetically modified bone marrow stromal cells ameliorate deficits in a rat model of stroke2009

    • Author(s)
      Hayase M
    • Journal Title

      J Cereb Blood Flow Metab 29

      Pages: 1409-1420

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new monoclonal antibody, A3B10, specific for astrocyte-lineage cells recognizes calmodulin-regulated spectrin-associated protein 1 (Camsap1)2009

    • Author(s)
      Yamamoto M
    • Journal Title

      J Neurosci Res 87

      Pages: 503-513

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Practical induction system for dopamine-producing cells from bone marrow stromal cells using spermine-pullulan-mediated reverse transfection method2009

    • Author(s)
      Nagane K, Kitada M
    • Journal Title

      Tissue Eng Part A 15

      Pages: 1655-1665

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 神経の再生2008

    • Author(s)
      北田容章、出澤真理(+7 perople)
    • Journal Title

      日本臨床 66(5)

      Pages: 921-925

    • Related Report
      2009 Final Research Report
  • [Journal Article] 神経の再生2008

    • Author(s)
      Kitada M
    • Journal Title

      日本臨床 66

      Pages: 921-925

    • Related Report
      2008 Annual Research Report
  • [Presentation] 失われたグリア前駆細胞を求めて-DM-20 mRNA発現細胞に注目して-2010

    • Author(s)
      北田容章・出澤真理
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      盛岡市
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 成体中枢神経系における新たな前駆細胞の探索-内在性前駆細胞の賦活化による神経疾患治療を目指して-2009

    • Author(s)
      北田容章
    • Organizer
      第34回峠の会
    • Place of Presentation
      東蒲原群
    • Year and Date
      2009-07-31
    • Related Report
      2009 Final Research Report
  • [Presentation] 成体中枢神経系における新た無前駆細胞の探索-内在性前駆細胞の賦活化による神経疾患治療を目指して-2009

    • Author(s)
      北田容章
    • Organizer
      第34回峠の会
    • Place of Presentation
      東蒲原群
    • Year and Date
      2009-07-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] 同種動物作成抗体による多重染色法-tyramideとmonovalent抗体の比較-2009

    • Author(s)
      北田容章・出澤真理
    • Organizer
      日本顕微鏡学会第65回学術講演会
    • Place of Presentation
      仙台市
    • Year and Date
      2009-05-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 同種動物作成抗体による多重染色法-tyramide と monovalent 抗体の比較-2009

    • Author(s)
      北田容章
    • Organizer
      日本顕微鏡学会第65回学術講演会
    • Place of Presentation
      仙台市
    • Year and Date
      2009-05-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 成体中枢神経に存在する幼若細胞の探索-内在性前駆細胞の賦活化による神経疾患治療を目指して-2009

    • Author(s)
      北田容章・出澤真理
    • Organizer
      第114回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岡山市(シンポジウム)
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 成体中枢神経に存在する幼若細胞の探索-内在性前駆細胞の賦活化による神経疾患治療を目指して-2009

    • Author(s)
      北田容章
    • Organizer
      第114回日本解剖学会総会・全国学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Notch regulation of the spinal progenitor fate in the adult rodent.2008

    • Author(s)
      Kitada M, Dezawa M
    • Organizer
      The 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry (ICHC2008)
    • Place of Presentation
      Gdansk, Poland
    • Year and Date
      2008-08-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Lectin-binding pattern reveals involvement of glycoconjugates and carbohydrate antigens in neural stem cell niche.2008

    • Author(s)
      Kitada M, Dezawa M
    • Organizer
      The 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry (ICHC2008)
    • Place of Presentation
      Gdansk, Poland
    • Year and Date
      2008-08-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Notch regulation of the spinal progenitor fate in the adult rodent2008

    • Author(s)
      Kitada M
    • Organizer
      The 13^<th> Congress of the International Federation of Societies for Histochemistry and Cytochemistry (ICHC2008)
    • Place of Presentation
      Gdansk, Poland
    • Year and Date
      2008-08-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] Lectin-binding pattern reveals involvement of glycoconjugates and carbohydrate antigens in neural stem cell niche2008

    • Author(s)
      Kitada M
    • Organizer
      The 13^<th> Congress of the International Federation of Societies for Histochemistry and Cytochemistry (ICHC2008)
    • Place of Presentation
      Gdansk, Poland
    • Year and Date
      2008-08-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 成体脊髄の新たなグリア前駆細胞の同定と、その細胞分裂・分化にかかわる分子の探索2008

    • Author(s)
      北田容章・出澤真理
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      千代田区
    • Year and Date
      2008-07-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 成体脊髄の新たなグリア前駆細胞の同定と、その細胞分裂・分化に関わる分子の探索2008

    • Author(s)
      北田容章
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2008-07-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] 同種動物作成抗体による多重染色-tyramide反応を用いて-2008

    • Author(s)
      北田容章・出澤真理
    • Organizer
      日本顕微鏡学会第64回学術講演会
    • Place of Presentation
      京都市
    • Year and Date
      2008-05-23
    • Related Report
      2009 Final Research Report 2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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