Reincorporation of cathepsin D in neurons of mouse brain.

• Project/Area Number: 20790162
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Niigata University
• Principal Investigator: SHIBATA Masahiro Niigata University, 医歯学系, 准教授 (10343253)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: リソソーム / カテプシン / ライソゾーム病 / セロイドリポフスチノーシス / カテプシンD / トランスポゾン / ミクログリア / NCL

Research Abstract

Central nervous system specific cathepsin D (CD) deficient mice (CD:NesCre) ied around P40 by seizures and their phenotype was similar to neuronal ceroid lipofuscinosis. We noticed that CD protein was detected in neurons of CD:NesCre mice by immunohistochemistry, although CD mRNA was not expressed in the neurons. These results indicate that CD:NesCre neurons receive CD from other type of cells. We have also developed the chick experimental model to examine the transport system of lysosomal enzymes and established the stable expression system of transgenes into chick genome.

Research Products

• [Journal Article] LC3, a microtubule-associated proteinlA/B light chain3, is involved in cytoplasmic lipid droplet formation. (2010)
  • Author(s): Shibata M, et al
  • Journal Title: Biochem Biophys Res Commun. 393 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Atg9A Protein, an Autophagy-related Membrane Protein, Is Localized in the Neurons of Mouse Brains. (2010)
  • Author(s): Tamura H, et al
  • Journal Title: J Histochem Cytochem 58 Pages: 443-453
  • Peer Reviewed
• [Journal Article] Autophagic neuron death (2009)
  • Author(s): Uchiyama Y, Koike M, Shibata M, Sasaki M.
  • Journal Title: Methods Enzymol 453 Pages: 33-51
• [Journal Article] Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation (2009)
  • Author(s): Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, Dono K, Doki Y, Kominami E, Sugioka A, Monden M
  • Journal Title: Uchiyama Y. Autophagy 5(3) Pages: 351-60
  • Peer Reviewed
• [Journal Article] The MAP1-LC3 conjugation system is inved in lipid droplet formation (2009)
  • Author(s): Shibata M, et al
  • Journal Title: Biochem Biophys Res Commun. 382 Pages: 419-423
  • Peer Reviewed
• [Journal Article] The MAP1-LC3 conjugation System is involved in lipid droplet formation (2009)
  • Author(s): Shibata M., et.al.
  • Journal Title: Biochemical and biophysical research communications 382(2) Pages: 419-423
  • Peer Reviewed
• [Journal Article] Autephagic neuron death (2009)
  • Author(s): Uchiyama Y., et.al.
  • Journal Title: Methods in enzymology 453 Pages: 33-51
  • Peer Reviewed
• [Journal Article] Participation of autophagy in the initiation of graft dysfunctiOn after rat liver transplantation (2009)
  • Author(s): Gotoh K., et.al.
  • Journal Title: Autophagy 5(3) Pages: 351-360
  • Peer Reviewed
• [Journal Article] Very-high-dosealpha-tocopherolsupplementatilp increases bloodpressure and causes possible adverse central nervous system effects in stroke-prone spontaneously hynertensiye rats (2009)
  • Author(s): Miyamoto K., et.al.
  • Journal Title: J Neurosci Res 87(2) Pages: 556-566
  • Peer Reviewed
• [Journal Article] Autophagic neuron death in neonatal brain ischemia/hypoxia (2008)
  • Author(s): Uchiyama Y, Koike M, Shibata M.
  • Journal Title: Autophagy 4(4) Pages: 404-8
  • Peer Reviewed
• [Journal Article] Hepatic gap junctions in thehepatocarcinogen-resistant DRH rat (2008)
  • Author(s): Gotow T., et.al.
  • Journal Title: Histochem Cell Biol 130(3) Pages: 583-594
  • Peer Reviewed
• [Journal Article] Autophagpphysiology and pathophysiology (2008)
  • Author(s): Uchiyama Y., et.al.
  • Journal Title: Histochem Cell Biol 129(4) Pages: 407-420
  • Peer Reviewed
• [Presentation] 鶏胚での条件的遺伝子発現抑制法 (2010)
  • Author(s): 柴田昌宏、佐藤昇
  • Organizer: 第115回日本解剖学会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 2010-03-28
• [Presentation] 鶏胚での条件的遺伝子発現抑制法 (2010)
  • Author(s): 柴田昌宏、佐藤昇
  • Organizer: 日本解剖学会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 2010-03-28
• [Presentation] Efficient gene transfer to developing chick astrocytes by Tol2 transposition (2009)
  • Author(s): M. Shibata, R. Inoue, N. Sato
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] Efficient gene transfer to developing chick astrocytes by To12 transposition (2009)
  • Author(s): 柴田昌宏、井上良介、佐藤昇
  • Organizer: 日本神経科学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] オートファジーと脂肪滴について (2009)
  • Author(s): 柴田昌宏
  • Organizer: 第114回日本解剖学会全国学術総会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2009-03-28
• [Presentation] Lysosomal cathepsin D in neurons is in part delivered from glial cells (2008)
  • Author(s): M. Shibata, M. Koike, Y. Uchiyama
  • Organizer: 第38回米国神経科学会
  • Place of Presentation: ワシントンDC、米国
  • Year and Date: 2008-11-17
• [Presentation] Lysosomal cathepsin D in neurons is in part delivered from glial cells (2008)
  • Author(s): 柴田昌宏
  • Organizer: Neuroscience2008
  • Place of Presentation: Washington.DC
  • Year and Date: 2008-11-17
• [Presentation] Reincorporation of cathepsin D in neurons of mouse brain (2008)
  • Author(s): M. Shibata, M. Koike, Y. Uchiyama
  • Organizer: 第34回日本神経科学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-09
• [Presentation] Reincorporation of cathepsin D in neurons of mouse brain (2008)
  • Author(s): 柴田昌宏
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-09