Molecular mechanism of synapsis checkpoint in mammalian meiosis

• Project/Area Number: 20790169
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Fujita Health University
• Principal Investigator: KOGO Hiroshi Fujita Health University, 総合医科学研究所, 助教 (20282387)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 減数分裂 / 不妊症 / 不育症 / チェックポイント / 対合 / ノックアウトマウス / 不妊 / リン酸化 / 無精子 / 流産 / チェックプイント / ノックアトマウス / 雌雄差

Research Abstract

Meiosis is a special type of cell division producing oocytes and sperms. The failure in the process of meiosis causes infertility and miscarriage in human reproduction. To avoid such problems, there is a surveillance mechanism called meiotic checkpoint, although its molecular mechanism has been totally unknown, especially in mammals. In this study, the function of a meiosis-specific gene HORMAD1 was analyzed by generating gene-targeted mice. As a result, HORMAD1 was identified as the first gene required for the meiotic checkpoint in mammals.

Research Products

• [Journal Article] Screening of genes involved in chromosome segregation during meiosis I: towards the identification of genes responsible for infertility in humans. (2010)
  • Author(s): Kogo, H., Kowa- Sugiyama, H., Yamada, K., Bolor, H., Tsutsumi, M., Ohye, T., Inagaki, H., Taniguchi, M., Toda, T., Kurahashi, H.
  • Journal Title: J. Hum. Genet. 55(5) Pages: 293-299
  • NAID: 10030734983
  • Peer Reviewed
• [Journal Article] Paternal origin of the de novo constitutional t(11;22)(q23;q11). (2010)
  • Author(s): Ohye, T., Inagaki, H., Kogo, H., Tsutsumi, M., Kato, T., Tong, M., Macville, M.V., Medne, L., Zackai, E.H., Emanuel, B.S. Kurahashi, H.
  • Journal Title: Eur. J. Hum. Genet. (in press)
  • Peer Reviewed
• [Journal Article] Screening of genes involved in chromosome segregation during meiosis I : towards the identification of genes responsible for infertility in humans (2010)
  • Author(s): Kogo, H.et al.
  • Journal Title: Journal of Human Genetics (in press)
  • NAID: 10030734983
  • Peer Reviewed
• [Journal Article] Paternal origin of the de novo constitutional t(11;22)(q23;q11) (2010)
  • Author(s): Ohye, T.et al.
  • Journal Title: European Journal of Human Genetics (in press)
  • Peer Reviewed
• [Journal Article] Mutations of the SYCP3 gene in women with recurrent pregnancy loss. (2009)
  • Author(s): Bolor, H., Mori, T., Nishiyama, S., Ito, Y., Hosoba, E., Inagaki, H., Kogo, H., 他11名
  • Journal Title: Am. J. Hum. Genet. 84 Pages: 14-20
  • Peer Reviewed
• [Journal Article] Chromosomal instability mediated by non-B DNA: Cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans. (2009)
  • Author(s): Inagaki, H., Ohye, T., Kogo, H., Kato, T., Bolor, H., Taniguchi, M., Shaikh, T.H., Emanuel, B.S., Kurahashi, H.
  • Journal Title: Genome Res. 19 Pages: 191-198
  • Peer Reviewed
• [Journal Article] Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells. (2009)
  • Author(s): Kurahashi, H. Inagaki, H., Kato, T., Hosoba, E., Kogo, H., Ohye, T., Tsutsumi, M., Bolor, H., Tong, M., Emanuel, B.S.
  • Journal Title: Hum. Mol. Genet. 18 Pages: 3397-3406
  • Peer Reviewed
• [Journal Article] Recent advance in our understanding of the molecular nature of chromosomal abnormalities. (2009)
  • Author(s): Kurahashi, H., Bolor, H., Kato, T., Kogo, H., Tsutsumi, M., Inagak i, H., Ohye, T.
  • Journal Title: J Hum Genet. 54 Pages: 253-260
  • NAID: 10030730167
• [Journal Article] Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells (2009)
  • Author(s): Kurahashi, H.et al.
  • Journal Title: Human Molecular Genetics 18 Pages: 3397-3406
  • Peer Reviewed
• [Journal Article] Recent advance in our understanding of the molecular nature of chromosomal abnormalities (2009)
  • Author(s): Kurahashi, H.et al.
  • Journal Title: Journal of Human Genetics 54 Pages: 253-260
• [Journal Article] Mutations of the SYCP3 gene in women with recurrent pregnancy loss (2009)
  • Author(s): Bolor, H.
  • Journal Title: American Journal of Human Genetics 84 Pages: 14-20
  • Peer Reviewed
• [Journal Article] Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. (2008)
  • Author(s): Nishizawa, H., Pryor-Koishi, K., Suzuki, M., Kato, T., Kogo, H., Sekiya, T., Kurahashi, H., Udagawa, Y.
  • Journal Title: Mol. Hum. Reprod. 14 Pages: 595-602
  • Peer Reviewed
• [Journal Article] Chromosomal instability mediated by non- B DNA : Cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans (2008)
  • Author(s): Inagaki, H.
  • Journal Title: Genome Research 19 Pages: 191-198
  • Peer Reviewed
• [Journal Article] Increased levels of pregnancy- associated plasma protein- A2 in the serum of pre- eclamptic patients (2008)
  • Author(s): Nishizawa, H.
  • Journal Title: Molecular Human Reproduction 14 Pages: 595-602
  • Peer Reviewed
• [Presentation] HORMAD1は哺乳類の減数分裂における対合チェックポイントに必須である. (2010)
  • Author(s): 向後寛、堤真紀子、大江瑞恵、稲垣秀人、倉橋浩樹
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] HORMAD1は哺乳類の減数分裂における対合チェックポイントに必須である (2010)
  • Author(s): 向後寛
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation] HORMAD1は哺乳類雌の対合チェックポイントに必須である. (2009)
  • Author(s): 向後寛、堤真紀子、大江瑞恵、稲垣秀人、倉橋浩樹
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] HORMAD1は哺乳類雌の対合チェックポイントに必須である (2009)
  • Author(s): 向後寛
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 哺乳類の減数分裂におけるHORMAD1の機能と減数分裂チェックポイントの性差. (2009)
  • Author(s): 向後寛, ボロルハスバイラ, 堤真紀子, 大江瑞恵, 稲垣秀人, 倉橋浩樹
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] 哺乳類の減数分裂におけるHORMAD1の機能と減数分裂チェックポイントの性差 (2009)
  • Author(s): 向後寛
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] 哺乳類の減数分裂におけるHORMAD1の機能とチェックポイントの性的二型性. (2008)
  • Author(s): 向後寛、ボロルハスバイラ、堤真紀子、大江瑞恵、稲垣秀人、倉橋浩樹
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 哺乳類の減数分裂におけるHORMAD1の機能とチェックポイントの性的二型性 (2008)
  • Author(s): 向後寛
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Remarks]
  • URL: http://info.fujita-hu.ac.jp/~genome/mg/
• [Remarks]
  • URL: http://info.fujita-hu.ac.jp/~genome/mg/
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/~rgenome/mg/