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Analysis of physiological roles of ion channels in ER membrane

Research Project

Project/Area Number 20790173
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionTohoku University

Principal Investigator

MURATA Yoshimichi  Tohoku University, 大学院・医学系研究科, 助教 (60455780)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords生体膜 / イオンチャネル / 小胞体膜
Research Abstract

We prepared nuclear envelopes (NEs) from mouse pancreatic acinar cells and observed MaxiK currents in the membrane subjected to the patch-clamp technique. The results indicate that the MaxiK channels are oriented in the ER membrane as their usual extracellular side faces to cytosol. These observation is inconsistent with the conventional understandings of the protein transport process. In this study, we examined the orientation of MaxiK channel in the ER membrane of the pancreatic acinar cells.
We performed an immunofluorescent staining in our NE preparation using anti-Calnexin antibodies. Calnexin, an ER resident membrane protein, direscts C-terminus to the cytosol and N-terminus to the lumen. The antibody which binds to N-terminus of Calnexin showed the signal only when NE membrane was permeablilized. It supports the notion that the orientaion of our NE preparation itself would be intact. Collectively, we suggest that MaxiK channels of the mouse pancreatic acinar cells have unconventional orientation in the ER membrane.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (9 results)

All 2009 2008

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Voltage-sensing phosphatase : actions and potentials2009

    • Author(s)
      Yasushi Okamura
    • Journal Title

      Journal of Physiology 587

      Pages: 513-520

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Asymmetry of Rb+ conduction emerged under bi-ionic conditions in epithelial Maxi-K+ channels2008

    • Author(s)
      Ohsaga A., Murata Y., Kondo Y., Hira R., Maruyama Y.
    • Journal Title

      Journal of. Physiological Science 58

      Pages: 363-369

    • NAID

      130004466936

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetry of Rb^+ conduction emerged under bi-ionic conditions in epithelial Maxi-K^+ channels2008

    • Author(s)
      Atsushi Ohsaga
    • Journal Title

      J. Physiol. Sci. 58

      Pages: 363-369

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 電位感受性酵素Ci-VSPは、脱分極により活性化される2009

    • Author(s)
      村田喜理
    • Organizer
      第37回薬物活性シンポジウム
    • Place of Presentation
      東北薬科大学
    • Year and Date
      2009-10-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] A catalogue of nuclear membrane channels and their role in pancreatic acinar cells IUPS2009

    • Author(s)
      丸山芳夫、村田喜理
    • Organizer
      第36回国際生理学会
    • Place of Presentation
      京都
    • Year and Date
      2009-07-30
    • Related Report
      2009 Final Research Report
  • [Presentation] Role of glysines and prolines on the putative permeation path of TRPV1 and TRPM8 channels2009

    • Author(s)
      村田喜理
    • Organizer
      IUPS第36回国際生理学会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] A catalogue of nuclear membrane channels and their role in pancreaticacinar cells2009

    • Author(s)
      丸山芳夫
    • Organizer
      IUPS第36回国際生理学会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] K+-Rb+共イオン条件下での非対称性Rb+電流コンダクタンス2008

    • Author(s)
      大佐賀敦、村田喜理、平理一郎、丸山芳夫
    • Organizer
      東北生理談話会
    • Place of Presentation
      弘前大学
    • Year and Date
      2008-10-08
    • Related Report
      2009 Final Research Report
  • [Presentation] K^+-Rb^+共イオン条件下での非対称性Rb+電流コンダクタンス2008

    • Author(s)
      丸山芳夫ら
    • Organizer
      東北生理談話会
    • Place of Presentation
      弘前大学医学部
    • Year and Date
      2008-10-08
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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