| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Transverse-tubule (T-tubule) is invagination of plasma membrane, only found in ventricular and skeletal myocytes, and have important role for excitation and contraction coupling. To identify molecules involved in formation and maintain of T-tubule structure in cardiac myocyte, mRNA expression of candidate genes were determined by Northern blotting. Among the genes tested, Pacsin3, a member of F-BAR protein family, was highly expressed in adult ventricular myocyte. To reveal the functions of Pacsin3, several deletion mutants was transiently transfected in COS7 cell. C-terminal deletion mutant of Pacsin3 was co-localized with alpha-tubulin, and changed form of microtubule network.
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