The regulatory mechanism of claudins for paracellular pathway of the human amniotic epithelium.

• Project/Area Number: 20790177
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Keio University
• Principal Investigator: KOBAYASHI Ken Keio University, 大学院・農学研究院, 助教 (30449003)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 羊膜 / タイトジャンクション / クローディン / 羊水 / 傍細胞経路 / 絨毛膜羊膜炎 / 早産 / 分娩 / 上皮層 / クローデイン

Research Abstract

The amniotic epithelium is in direct contact with the amniotic fluid and regulates fluid flux via paracellular pathway by tight junctions. In this study, we investigated the regulatory mechanism of tight junctions and claudins as the basic research for premature birth and chorioamnionitis. The results have been shown that several physiological active substances such as hormone and inflammatory cytokines regulate the permeability of amniotic tight junctions through claudins.

Research Products

• [Journal Article] Progesterone maintains amniotic tight junctions during midpregnancy in mice. (2011)
  • Author(s): Kobayashi K, Miwa H, Yasui M
  • Journal Title: Molecular and Cellular Endocrinology 337 Pages: 36-42
  • Peer Reviewed
• [Journal Article] Apoptosis in mouse amniotic epithelium is induced by activated macrophages through the TNF receptor type 1/TNF pathway. (2011)
  • Author(s): Kobayashi K, Umezawa K, Yasui M
  • Journal Title: Biology of Reproduction 84 Pages: 248-254
  • Peer Reviewed
• [Journal Article] Progesterone maintains amniotic tight junctions during midpregnancy in mice. (2011)
  • Author(s): Kobayashi K.
  • Journal Title: Molecular and Cellular Endocrinology Volume: 337 Pages: 36-42
  • Peer Reviewed
• [Journal Article] Apoptosis in mouse amniotic epithelium is induced by activated macrophages through the TNF receptor type 1/TNF pathway. (2011)
  • Author(s): Kobayashi K.
  • Journal Title: Biology of Reproduction Volume: 84 Pages: 248-54
  • Peer Reviewed
• [Journal Article] Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice. (2010)
  • Author(s): Kobayashi K, Yasui M
  • Journal Title: Cell and Tissue Research 342 Pages: 307-316
  • Peer Reviewed
• [Journal Article] Inflammatory mediators weaken the amniotic membrane barrier through disruption of tight junctions. (2010)
  • Author(s): Kobayashi K, Miwa H, Yasui M
  • Journal Title: The Journal of Physiology 588 Pages: 4859-4869
  • Peer Reviewed
• [Journal Article] Expression and distribution of tight junction proteins in human amnion during late pregnancy. (2010)
  • Author(s): Kobayashi K, Kadohira I, Tanaka M, Yoshimura Y, Ikeda K, Yasui, M
  • Journal Title: Placenta 31 Pages: 158-162
  • Peer Reviewed
• [Journal Article] Inflammatory mediators weaken the amniotic membrane barrier throngh disruption of tight junctions. (2010)
  • Author(s): Kobayashi K.
  • Journal Title: The Journal of Physiology Volume: 588 Pages: 4859-4869
  • Peer Reviewed
• [Journal Article] Expression and distribution of tight junction proteins in human amnion during late pregnancy. (2010)
  • Author(s): KEN KOBAYASHI
  • Journal Title: Placenta 31 Pages: 34-39
  • Peer Reviewed
• [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy. (2009)
  • Author(s): Kobayashi K, Inai T, Shibata Y, Yasui, M
  • Journal Title: Placenta 30 Pages: 840-847
  • Peer Reviewed
• [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy. (2009)
  • Author(s): KEN KOBAYASHI
  • Journal Title: Placenta 30 Pages: 840-847
  • Peer Reviewed
• [Journal Article] Clinical application of in situ tissue engineering using a scaffolding technique for reconstruction of the larynx and trachea (2008)
  • Author(s): KOICHI OMORI
  • Journal Title: Annals of Otology, Rhinology & Laryngology 117 Pages: 673-678
  • Peer Reviewed
• [Journal Article] Regeneration of the trachea using bio-engineered scaffold with adipose-derived stem cells (2008)
  • Author(s): TERUHISA SUZUKI
  • Journal Title: Annals of Otology, Rhinology & Laryngology 117 Pages: 453-463
  • Peer Reviewed
• [Journal Article] The effect of fibroblasts on epithelial regeneration on the surface of the bioengineered trachea (2008)
  • Author(s): YUKIO NOMOTO
  • Journal Title: Annals of Otology, Rhinology & Laryngology 117 Pages: 59-64
  • Peer Reviewed
• [Remarks] ホームページ等