Regulation of physiological functions by magnesium ions in white adipocytes

• Project/Area Number: 20790179
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Tokyo Medical University
• Principal Investigator: INOUE Hana Tokyo Medical University, 医学部, 助教 (20390700)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分子 / 細胞生理学 / イオンチャネル / 白色脂肪細胞 / 生理機能

Research Abstract

In the present study, we have established the methods to prepare white adipocytes suitable for various physiological experiments. In this preparation, it was clarified that the volume-sensitive chloride channel is expressed and involved in volume regulation in white adipocytes. Expression level of this channel was decreased in white adipocytes from diabetic animals. Moreover, it was also revealed that white adipocytes express MIC channel which is regulated by both intracellular and extracellular magnesium ions.

Research Products

• [Journal Article] Volume-sensitive outwardly rectifying chloride channel in white adipocytes from normal and diabetic mice. (2010)
  • Author(s): H. Inoue, N. Takahashi, Y. Okada, M. Konishi
  • Journal Title: Am. J. Physiol. Cell Physiol. 298
  • Peer Reviewed
• [Journal Article] Volume-sensitive outwardly rectifying chloride channel in white adipocy-tes from normal and diabetic mice. (2010)
  • Author(s): Inoue H, Takahashi N, Okada Y, Konishi M
  • Journal Title: American Journal of Physiology-Cell Physiology 298 Pages: 900-909
  • Peer Reviewed
• [Presentation] Volume-sensitive chloride channel activity in freshly isolated white adip-ocytes from normal and diabetic mice. (2009)
  • Author(s): Inoue H, Takahashi N, Okada Y, Konishi M
  • Organizer: 36^<th> International Congress of Physiological Sciences
  • Place of Presentation: Kyoto,Japan
  • Year and Date: 2009-07-31
• [Presentation] 白色脂肪細胞における容積感受性クロライドチャネルの機能的発現と容積調節への関与 (2009)
  • Author(s): 井上華、小西真人
  • Organizer: 東京医科大学医学会総会
• [Presentation] Volume-sensitive chloride channel activity in freshly isolated white adipocytes. (2008)
  • Author(s): H. Inoue, N. Takahashi, Y. Okada, M. Konishi
  • Organizer: 日本生理学会大会
• [Presentation] Volume-sensitive chloride channel activity in freshly isolated white adipocytes from normal and diabetic mice. (2008)
  • Author(s): H. Inoue, N. Takahashi, Y. Okada, M. Konishi
  • Organizer: International Congress of Physiological Sciences