Analysis of coupling mechanism of voltage-gated H^+ channel and NADPH oxidase

• Project/Area Number: 20790189
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Osaka University
• Principal Investigator: OKOCHI Yoshifumi Osaka University, 医学系研究科, 助教 (90435818)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 電位依存性プロトンチャネル / 好中球 / 活性酸素 / NADPHオキシダーゼ / 貪食細胞

Research Abstract

We found that voltage-gated proton channel, VSOP, regulates reactive oxygen species (ROS) generation in neutrophils, which have the ability of killing pathogens. We also found the possibility that VSOP and NADPH oxidase interact physically and that both molecules are present in same location of neutrophils. These results suggest that VSOP can regulate intracellular/extracellular proton and membrane potential properly by interaction with NADPH oxidase and then regulate ROS generation.

Research Products

• [Journal Article] VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification (2010)
  • Author(s): El Chemaly A, Okochi Y, Sasaki M, Arnaudeau S, Okamura Y, Demaurex N.
  • Journal Title: Journal of experimental medicine Vol.207 Pages: 129-139
  • Peer Reviewed
• [Journal Article] Functionality of the voltage-gated proton channel truncated in S4 (2010)
  • Author(s): Sakata S, Kurokawa T, Norholm MH, Takagi M, Okochi Y, von Heijne G, Okamura Y.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Vol.107 Pages: 2313-2318
  • Peer Reviewed
• [Journal Article] Functionality of the voltage-gated proton channel truncated in S4. (2010)
  • Author(s): Sakata S, Kurokawa T, Nerholm MH, Takagi M, Okochi Y, von Heijne G, Okamura Y.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 107 Pages: 2313-2318
  • Peer Reviewed
• [Journal Article] VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification. (2010)
  • Author(s): El Chemaly A, Okochi Y, Sasaki M, Arnaudeau S, Okamura Y, Demaurex N.
  • Journal Title: Journal of experimental medicine 207 Pages: 129-139
  • Peer Reviewed
• [Journal Article] Voltage-gated proton channel is expressed on phagosomes (2009)
  • Author(s): Okochi Y, Sasaki M, Iwasaki H, Okamura Y.
  • Journal Title: Biochemical and Biophysical Research Communications Vol.382 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Voltage-gated proton channel is expressed on phagosomes. (2009)
  • Author(s): Okochi Y, Sasaki M, Iwasaki H, Okamura Y.
  • Journal Title: Biochemical and Biophysical Research Communications 382 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Larsson HP Multimeric nature of voltage-gated proton channels (2008)
  • Author(s): Koch HP, Kurokawa T, Okochi Y, Sasaki M, Okamura Y
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Vol.105 Pages: 9111-9116
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain MI, Iwasaki H, Okochi Y, Chahine M, Higashijima S, Nagayama K, Okamura Y.
  • Journal Title: Journal of Biological Chemistry Vol.283 Pages: 18248-18259
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain MI, Iwasaki H, Okoch Y, Chahine M, Higashijima S, Nagayama K, Okamura Y.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 18248-59
  • Peer Reviewed
• [Journal Article] Multimeric nature of voltage-gated proton channels (2008)
  • Author(s): Koch HP, Kurokawa T, Okochi Y, Sasaki M, Okamura Y, La rsson HP
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 105 Pages: 9111-6
  • Peer Reviewed
• [Presentation] Voltage-gated proton channel is expressed on phagosome (2009)
  • Author(s): Yoshifumi Okochi, Mari Sasaki, Yasushi Okamura
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2009-09-16
• [Presentation] Analyses of voltage-gated proton channel, VSOP, in phagocytes (2009)
  • Author(s): Y. Okochi, M. Sasaki, Y. Okamura
  • Organizer: The 36th Congress of the International Union of Physiological Sciences (IUPS2009)
  • Place of Presentation: Kyoto International Conference Center (Kyoto)
  • Year and Date: 2009-07-30
• [Presentation] Analyses of voltage-gated proton channel, VSOP, inphagocytes (2009)
  • Author(s): Y.Okochi, M.Sasaki, Y.Okamura
  • Organizer: the 36th Congress of the International Union of Phsioloical Sciences (IUPS2009)
  • Place of Presentation: Kyoto International Confe rence Center
  • Year and Date: 2009-07-30
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm