Localization mechanism of a Ca^<2+> pump modulator, phospholamban in cardiac sarcoplasmic reticulum

Research Project

Project/Area Number	20790208
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	General pharmacology
Research Institution	Yamaguchi University
Principal Investigator	HONDA Takeshi Yamaguchi University, 大学院・医学系研究科, 助教 (30457311)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	化学架橋 / 筋小胞体 / 心不全 / ホスホランバン / 心筋小胞体 / 免疫沈降 / アプタマー
Research Abstract	Phospholamban (PLN) is a regulator of the myocardiac contractility in cardiac sarcoplasmic reticulum (SR). Controling the subcellular localization of PLN leads to the prospect for new therapy of heart failure. In this study, it was suggested that the C-terminal region of PLN is critical for localization of PLN to SR. Based on this finding, the identification of a putative protein interacting to the C-terminal region of PLN was attempted. Furthermore, a novel PLN-targeted aptamer was successfully developed as the useful molecular tool for detection of the PLN-binding protein.

Report

(3 results)