| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Mammalian nuclear Dbf2-Related (NDR) kinases (NDR1/2, LATS1/2) play a role in cell proliferation, apoptosis and morphological changes. These kinases are regulated by mammalian sterile 20-like kinases (MSTs) and Mps one binder (MOB) 1. MST2 and MOB1 synergistically activate NDR1. Activated MST2 facilitates the tripartite complex formation of MOB1, MST2 and NDR1 in cells. The in vitro biochemical study demonstrates the phosphorylation of MOB1 by MST2 at several sites. Point mutation analysis revealed that phosphorylation of MOB1 is crucial for the activation of NDR1/2 and LATS1/2. We also demonstrated that MST2 suppressed RASSF6 induced cell death. And at the same time, adaptor protein RASSF6 inhibits MST2 dependent NDR kinase activation.
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