Characterization of the cellular oxygen sensing machinery
| Project/Area Number |
20790223
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
NAKAYAMA Koh Tokyo Medical and Dental University, 難治疾患研究所, 特任講師 (10451923)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 生体分子医学 / 低酸素応答 / 細胞死 / エネルギー代謝 / タンパク質複合体 / プロリン水酸化酵素 / PHD3 / PRP19 / タンパク質間相互作用 / プロリン水酸化 / タンパク質 / シグナル伝達 / プロテオーム / 免疫沈降 / 細胞培養 / 質量分析 |
|---|
| Research Abstract |
Organisms respond to hypoxia condition by regulating respiration and metabolism to maintain homeostasis. It is not clear yet what is the 'oxygen sensor' molecule in vivo. In the present study, we have characterized the hypoxia complex which is formed by prolyl-hydroxylase PHD3. As a result, we identified several different molecules which would play roles in oxygen sensing. PDH regulated the metabolism, while PRP19 suppressed the cell death in hypoxia treated cells.
|
Report
(3 results)
Research Products
(10 results)
