Identification and characterization of a new ligand of BLT2

• Project/Area Number: 20790232
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: OKUNO Toshiaki Kyushu University, 大学院・医学研究院・医化学分野, 助教 (60361466)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 生理活性脂質 / 受容体 / GPCR / 脂質 / BLT2 / ロイコトリエンB4 / 12-HHT / Gタンパク質

Research Abstract

I found that 12-hydroxy-heptadeca-trienoic acid (12-HHT) is a natural ligand for BLT2, which was originally identified as a low-affinity leukotriene B4 receptor. From analyses of BLT2 deficient mice, we found that BLT2 is involved in inflammatory bowel disease, and we found that helix 8 of BLT2 C-teriminal is involved in receptor trafficking to the plasma membrane.

Research Products

• [Journal Article] Helix 8 of leukotriene B4 type-2 receptor is required for the folding to pass the quality control in the endoplasmic reticulum (2009)
  • Author(s): Yasuda D, Okuno T, Yokomizo T, Hori T, Hirota N, Hashidate T, Miyano M, Shimizu T, Nakamura M
  • Journal Title: FASEB Journal 23(5) Pages: 1470-1481
• [Journal Article] ロイコトリエンと炎症・免疫 (2009)
  • Author(s): 奥野利明、横溝岳彦
  • Journal Title: 炎症と免疫 17(4) Pages: 373-378
• [Journal Article] Helix 8 of leukotriene B_4 type-2 receptor is required for the folding to pass the quality control in the endoplasmic reticulum. (2009)
  • Author(s): Yasuda D, Okuno T, et al.
  • Journal Title: Faseb J 23(5) Pages: 1470-1481
  • Peer Reviewed
• [Journal Article] ロイコトリエンと炎症・免疫 (2009)
  • Author(s): 奥野利明、横溝岳彦
  • Journal Title: 炎症・免疫 17(4) Pages: 373-378
• [Journal Article] Helix 8 of leukotriene B4 type-2 receptor is required for the folding to pass the quality control in the endoplasmic reticulum (2009)
  • Author(s): Yasuda D, Okuno T, et.al.
  • Journal Title: Faseb J (In press)
  • Peer Reviewed
• [Journal Article] 12(S)- Hydroxyheptadeca- 5Z, 8E, 10Etrienoic acid is a natural ligand for leukotriene B4 receptor 2 (2008)
  • Author(s): Okuno T, Iizuka Y, Okazaki H, Yokomizo T, Taguchi R, Shimizu T
  • Journal Title: J. Exp. Med. 205 Pages: 759-766
• [Journal Article] ロイコトリエンB4第二受容体(BLT2)の生体内リガンドは12-HHTである (2008)
  • Author(s): 奥野利明
  • Journal Title: 実験医学カレントトピック
• [Journal Article] 2(S)-Hydroxyheptadeca-5Z, 8E, 10E-trienoic acid is a natural ligand for leukotriene B4 receptor 2 (2008)
  • Author(s): Okuno T, et.al.
  • Journal Title: J. Exp. Me d 205(4) Pages: 759-766
  • Peer Reviewed
• [Journal Article] ロイコトリエンB4第二受容体BLT2の生体内リガンドは12-HHTである (2008)
  • Author(s): 奥野利明
  • Journal Title: 実験医学 26(11) Pages: 1752-1755
• [Presentation] Gタンパク質共役型受容体の細胞内移行の分子機序 (2009)
  • Author(s): 荒武良総、奥野利明、横溝岳彦
  • Organizer: 第4回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2009-08-08
• [Presentation] オーファンGPCRの新規脂質リガンド探索 (2009)
  • Author(s): 松延武彦、奥野利明、横溝岳彦
  • Organizer: 第4回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2009-08-08
• [Presentation] DIFICIENCY OF LEUKOTRIENE B4 RECEPTORS AFFECTS TUMOR METASTASIS (2009)
  • Author(s): Okazaki H, Matsunobu T, Okuno T, Tokomizo T
  • Organizer: 第4回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2009-08-08
• [Presentation] 12-HHT, a Metabolite of Cycloocygenase, is a natural Ligand for Leukotriene B4 Receptor 2 (2009)
  • Author(s): Okuno
  • Organizer: BLT2 Gordon Research Conference
  • Place of Presentation: ニューイングランド大学
  • Year and Date: 2009-06-08
• [Presentation] 12-HHT, a Metabolite of Cycloocygenase, is a natural Ligand for Leukotriene B4 Receptor 2, BLT2 (2009)
  • Author(s): Okuno T, et al.
  • Organizer: Gordon Research Conference
  • Place of Presentation: ニューイングランド大学
  • Year and Date: 2009-06-08
• [Presentation] 12-HHT is a natural ligand for leukotriene B4 receptor 2 (2009)
  • Author(s): Okuno
  • Organizer: BLT2 International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 東京
  • Year and Date: 2009-05-27
• [Presentation] 12-HHT is a natural ligand for leukotriene B4 receptor 2, BLT2 (2009)
  • Author(s): Okuno T, et al.
  • Organizer: International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 東京
  • Year and Date: 2009-05-27
• [Presentation] 12-HHT, a Metabolite of Cyclooxygenase, is a natural Ligand for Leukotriene B4 Receptor 2 (2009)
  • Author(s): Okuno
  • Organizer: BLT2 The 5 th Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 東京
  • Year and Date: 2009-05-25
• [Presentation] 12-HHT, a Metabolite of Cyclooxygenase, is a natural Ligand for Leukotriene B4 Receptor 2, BLT2 (2009)
  • Author(s): Okuno T, et al.
  • Organizer: The 5 th Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 東京
  • Year and Date: 2009-05-25
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2009)
  • Author(s): 奥野利明
  • Organizer: 日本生化学会九州支部
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-17
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2009)
  • Author(s): 奥野利明
  • Organizer: 日本生化学会九州支部(学術奨励賞受賞講演)
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-17
• [Presentation] ロイコトリエンB4第2受容体BLT2の内在性リガンドの同定 (2009)
  • Author(s): 奥野利明
  • Organizer: 生命科学系GCOEネットワーク・フォーラム2009
  • Place of Presentation: 東京
  • Year and Date: 2009-02-14
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2009)
  • Author(s): 奥野利明
  • Organizer: Gタンパク質特定領域・膜輸送複合体特定領域 合同若手ワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2009-01-30
• [Presentation] GPCRが有するHelix 8の小胞体搬出における意義 (2009)
  • Author(s): 安田大恭、奥野利明、横溝岳彦、清水孝雄、中村元直
  • Organizer: Gタンパク質特定領域・膜輸送複合体特定領域合同若手ワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2009-01-30
• [Presentation] 12-HHT, a Metabolite of Cyclooxygenase, is a Natural Ligand for Leukotriene B4 Receptor 2 (2008)
  • Author(s): Okuno
  • Organizer: BLT2 Japan/Korea Conference on Cellular Signaling
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-19
• [Presentation] 2-HHT, a Metabolite of Cyclooxygenase, is a Natural Ligand for Leuk otriene B4 Receptor 2, BLT2 (2008)
  • Author(s): Toshiaki Okuno
  • Organizer: Japan/Korea Conference on Cellular Signaling
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-19
• [Presentation] ロイコトリエンB4第一受容体BLT1と血小板活性化因子受容体PAFRの脱感作機構 (2008)
  • Author(s): 荒武良総、奥野利明、横溝岳彦
  • Organizer: 第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2008)
  • Author(s): 奥野利明
  • Organizer: 第81回日本生化学会大会合同大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Generation and characterization of a novel high-affinity monoclonal antibody for FLAG tag (2008)
  • Author(s): Sasaki, F, Onohara, N, Okuno, T, Yokomizo T
  • Organizer: 第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 12(S)-Hydroxyheptadeca-5Z, 8E, 10E-trienoic acid is a natural ligand for leukotriene B4 receptor 2 (2008)
  • Author(s): Okuno
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Dificiency of leukotriene B4 receptors affects tumor metastasis (2008)
  • Author(s): Okazaki, H., Okuno, T., Yokomizo T
  • Organizer: The 2nd Global COE International Symposium joint with the 18th Hot Spring Harbor Symposium of Medical Institute of Bioregulation
  • Place of Presentation: 福岡
  • Year and Date: 2008-11-09
• [Presentation] ロイコトリエンB4第二受容体(BLT2)の生体内リガンドの同定 (2008)
  • Author(s): 奥野利明
  • Organizer: 東京呼吸器リサーチフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2008-11-01
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2008)
  • Author(s): 奥野利明
  • Organizer: 特定領域G蛋白質シグナル・班会議
  • Place of Presentation: 越後湯沢
  • Year and Date: 2008-09-19
• [Presentation] ロイコトリエンB4第一受容体BLT1の脱感作機構の解析 (2008)
  • Author(s): 荒武良総、奥野利明、横溝岳彦
  • Organizer: 特定領域G蛋白質シグナル・班会議
  • Place of Presentation: 越後湯沢
  • Year and Date: 2008-09-19
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2008)
  • Author(s): 奥野利明、横溝岳彦
  • Organizer: 特定領域G蛋白質シグナル班会議
  • Place of Presentation: 越後湯沢
  • Year and Date: 2008-09-19
• [Presentation] ロイコトリエンB4第二受容体(BLT2)の生体内リガンドはシクロオキシゲナーゼ産物12-HHTである (2008)
  • Author(s): 奥野利明
  • Organizer: 第2回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2008-08-10
• [Presentation] 2つのLeukotrieneB4受容体と癌の転移・浸潤 (2008)
  • Author(s): 岡崎寛士、奥野利明、横溝岳彦
  • Organizer: 第2回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2008-08-09
• [Presentation] 高感度抗FLAGモノクローナル抗体の樹立 (2008)
  • Author(s): 佐々木文之、小野原直哉、奥野利明、横溝岳彦
  • Organizer: 第2回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2008-08-09
• [Presentation] Gタンパク質共役型受容体の脱感作と細胞内移行の分子機序 (2008)
  • Author(s): 荒武良総、奥野利明、横溝岳彦
  • Organizer: 第2回GCOE理医連携リトリート
  • Place of Presentation: 熊本
  • Year and Date: 2008-08-09
• [Presentation] ロイコトリエンB4第二受容体(BLT2)の生体内リガンドは12-HHTである (2008)
  • Author(s): 奥野利明
  • Organizer: 第50回日本脂質生化学会
  • Place of Presentation: 徳島
  • Year and Date: 2008-06-06
• [Presentation] ロイコトリエンB4第二受容体(BLT2)の生体内リガンドはシクロオキシゲナーゼ産物12-HHTである (2008)
  • Author(s): 奥野利明
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-18
• [Presentation] ロイコトリエンB4第2受容体BLT2の内在性リガンドの同定 (2008)
  • Author(s): 奥野利明、横溝岳彦
  • Organizer: 生命科学系GCOEネットワーク・フォーラム2009
  • Place of Presentation: 東京
  • Year and Date: 2008-02-14
• [Presentation] ロイコトリエンB4第二受容体BLT2の生体内リガンドの同定 (2008)
  • Author(s): 奥野利明、横溝岳彦
  • Organizer: Gタンパク質特定領域・膜輸送複合体特定領域合同若手ワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2008-01-30
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002948/research.html
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002948/research.html
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002948/research.html