Regulation of cell polarity by a novel Par3-binding protein

• Project/Area Number: 20790233
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: KAMAKURA Sachiko Kyushu University, 生体防御医学研究所, 助教 (80398081)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞内シグナル伝達 / 細胞極性 / 上皮細胞 / ニューロン / 細胞性

Research Abstract

Cell polarization is crucial for a variety of processes in development. The Par3/Par6/aPKC (Par aPKC) complex is required for polarity establishment in several types of cell. During the process, the Par aPKC complex is targeted to the plasma membrane, which plays an important role in cell polarization. However, little is known about the mechanisms underlying proper subcellular localization of the complex. In the present study, we identify a novel Par3-binding protein, which recruits the Par aPKC complex to the plasma membrane for regulating mammalian epithelial cell polarity.

Research Products

• [Journal Article] Membrane phospholipid metabolism during phagocytosis in human neutrophils. (2010)
  • Author(s): Minakami, R., Maehara, Y., Kamakura, S., Kumano, O., Miyano, K., Sumimoto, H.
  • Journal Title: Genes Cells 15 Pages: 409-423
  • Peer Reviewed
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47 plays a crucial role in activation of the phagocyte NADPH oxidase (2009)
  • Author(s): Taura, M., Miyano, K., Minakami, R., Kamakura, S., Takeya, R., Sumimoto, H.
  • Journal Title: Biochem. J. 419 Pages: 329-338
  • Peer Reviewed
• [Journal Article] A region N-terminal to the tandem SH3 domain of p47^<phox> plays a crucial role in activation of the phagocyte NADPH oxidase (2008)
  • Author(s): Taura, M., Miyano, K., Minakami, R., Kamakura, S., Takeya, R., Sumimoto. H
  • Journal Title: Biochem. J 419 Pages: 329-338
  • Peer Reviewed
• [Journal Article] Membrane phospholipid metabolism during phagocytosis in human neutrophils.
  • Author(s): Minakami, R., Maehara, Y., Kamakura, S., Kumano, O., Miyano, K., Sumimoto, H.
  • Journal Title: Genes Cells (In press)
  • Peer Reviewed