Prediction of metabolic dynamics in human erythrocytes based on a large-scale mathematical model and its verification by metabolome analysis
Project/Area Number |
20790237
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General medical chemistry
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Research Institution | Keio University |
Principal Investigator |
YACHIE Ayako Keio University, 医学部, 助教 (10453549)
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Project Period (FY) |
2008 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | システム生物学 / シミュレーション / 血液保存 / メタボローム / 代謝解析 / 代謝シミュレーション / 細胞シミュレーション / 赤血球 / 保存血液 / アデノシン / 低酸素応答 |
Research Abstract |
Large-scale mathematical model of metabolism in human red blood cells predicted that high extracellular adenosine resulted in decrease in intracellular ATP levels, and that the reduction of cellular energy would be compensated by glycolytic activation through hypoxia-induced hemoglobin-transition. A simulation model that can reproduce the metabolic behavior in long and cold stored red blood cells in MAP solution was also developed. The MAP-stored model would contribute to high-throughput and low-cost screening of optimized stored condition.
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Report
(3 results)
Research Products
(31 results)
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[Journal Article] Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry Antioxid2010
Author(s)
Hattori, K., Kajimura, M., Hishiki, T., Nakanishi, T., Kubo, A., Nagahata, Y., Ohmura, M., Yachie-Kinoshita, A., Matsuura, T., Morikawa, T., Nakamura, T., Setou, M., Suematsu, M.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Hypotaurine is an energy-saving hepatoprotective compound against ischemia-reperfusion injury of the rat liver2010
Author(s)
Sakuragawa, T., Hishiki, T., Ueno, Y., Ikeda, S., Soga, T., Yachie-Kinoshita, A., Kajimura, M., Suematsu, M.
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Journal Title
J. Clin. Biochem. Nutr. 46(2)
Pages: 126-134
Related Report
Peer Reviewed
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[Journal Article] T-state stabilization of hemoglobin by nitric oxide to form a-nitrosyl heme causes constitutive release of ATP from human erythrocytes2010
Author(s)
Akatsu, T., Tsukada, K., Hishiki, T., Suganuma, K., Tanabe, M., Shimazu, M., Kitagawa, Y., Yachie-Kinoshita, A., Suematsu, M.
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Journal Title
Advances in Experimental Medicine and Biology 662
Pages: 109-114
Related Report
Peer Reviewed
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[Journal Article] Hypotaurine is an energy-saving hepatoprotective compound against ischemia-reperfusion injury of the rat liver.2010
Author(s)
Sakuragawa, T., Hishiki, T., Ueno, Y., Ikeda, S., Soga, T., Yachie-Kinoshita, A.,Kajimura, M.,Suematsu, M
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Journal Title
J Clin Biochem Nutr. 46(2)
Pages: 126-134
Related Report
Peer Reviewed
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[Journal Article] In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods2009
Author(s)
Nishino, T., Yachie-Kinoshita, A. * (* corresponding authour), Hirayama, A., Soga, T., Suematsu, M., Tomita, M.
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Journal Title
J Biotechnol 144(3)
Pages: 212-223
Related Report
Peer Reviewed
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[Presentation] Mathematical modeling of preserved red blood cells towards improvement of blood storage2009
Author(s)
Nishino, T., Yachie-Kinoshita, A., Hirayama, A., Soga, T., Suematsu, M. Tomita, M
Organizer
The 10^<th> International Conference on Systems Biology
Place of Presentation
Stanford, CA, USA
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[Presentation] 細胞における代謝の時空間シミュレーション2008
Author(s)
谷内江綾子, 川原仁志, 野田茂穂, 須永泰弘, 大日向大地, 横田秀夫, 末松誠
Organizer
BMB2008第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
Place of Presentation
神戸市
Year and Date
2008-12-11
Related Report
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