Mass production of enucleated erythrocyte from erythroid progenitor cell lines.
| Project/Area Number |
20790240
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HIROYAMA Takashi The Institute of Physical and Chemical Research, 細胞材料開発室, 専任研究員 (50373361)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ES / iPS / 赤血球 / 細胞株 / MEDEP / WAVE2 / Rac / ROCK / ips / 分化 / 前駆細胞株 / iPS分化 |
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| Research Abstract |
In this study, we developed a method that enables to produce enucleated erythrocyte efficiently from mouse erythroid progenitor cell lines (MEDEP). And it was indicated that erythroid membrane protein, GPC and Rac-WAVE2 signaling pathway have important rolls in enucleation process. In addition, a culture method using a ROCK inhibitor, by which hematopoietic cells can be induced from human ES or iPS cells, was developed. However, human erythroid progenitor cell line has not been established.
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Report
(3 results)
Research Products
(19 results)
