The molecular mechanism of maturation defects of RBP-J-deficient neurons.

• Project/Area Number: 20790244
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Research Institute, Shiga Medical Center
• Principal Investigator: TANIGAKI Kenji Research Institute, Shiga Medical Center, 遺伝子研究部門, 専門研究員 (70362473)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 発生医学 / Notch / RBP-J / 神経発生 / 大脳皮質 / conditional knockout

Research Abstract

Notch/RBP-Jシグナルは神経幹細胞の維持をはじめ、神経発生を多段階で制御することが知られていた。我々は、Notchシグナルの主要な伝達因子RBP-Jが神経細胞の成熟にも関与していることを見出した。蛍光蛋白質であるGPSの変異体であるVenusを用いてNotchシグナルを可視化することによって、その機能がRBP-Jの転写抑制によることを明らかにした。

Research Products

• [Journal Article] Sept5 deficiency exerts pleiotropic influence on affective behaviors and cognitive functions in mice. (2009)
  • Author(s): Suzuki G, Harper KM, Hiramoto T, Sawamura T, Lee M, Kang G, Tanigaki K, Buell M, Geyer MA, Trimble WS, Agatsuma S, Hiroi N.
  • Journal Title: Hum Mol Genet 18 Pages: 1652-60
• [Journal Article] RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis. (2009)
  • Author(s): Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
  • Journal Title: Dev Biol 332 Pages: 339-50
• [Journal Article] Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. (2009)
  • Author(s): arlen M, Meletis K, Goritz C, Darsalia V, Evergren E, Tanigaki K, Amendola M, Barnabe-Heider F, Yeung MS, Naldini L, Honjo T, Kokaia Z, Shupliakov O, Cassidy RM, Lindvall O, Frisen J.
  • Journal Title: Nat Neurosci 12 Pages: 259-67
• [Journal Article] RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis. (2009)
  • Author(s): Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
  • Journal Title: Dev Biol. 332 Pages: 339-350
  • Peer Reviewed
• [Journal Article] Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. (2009)
  • Author(s): Carlen M, Meletis K, Goritz C, Darsalia V, Evergren E, Tanigaki K, Amendola M, Barnabe-Heider F, Yeung MS, Naldini L, Honjo T, Kokaia Z, Shupliakov O, Cassidy RM, Lindvall O, Frisen J.
  • Journal Title: Nat Neurosci 12 Pages: 259-67
  • Peer Reviewed
• [Presentation] RBP-J promotes neuronal differentiation. (2009)
  • Author(s): Tanigaki K.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] RBP-J promotes neuronal differentiation. (2009)
  • Author(s): Tanigaki K.
  • Organizer: The Notch Meeting
  • Place of Presentation: ギリシア
  • Year and Date: 2009-09-25
• [Presentation] RBP-J promotes neuronal differentiation. (2009)
  • Author(s): Tanigaki K.
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] RBP-Jの神経機能における役割の解析 (2008)
  • Author(s): 谷垣健二
  • Organizer: 第3回Notch研究会
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2008-07-17
• [Presentation] BP-Jの神経機能における役割の解析 (2008)
  • Author(s): 谷垣健二
  • Organizer: 第3回Notch研究会
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2008-07-17
• [Remarks]
  • URL: http://www.shigamed.jp/staff/tanigaki.html
• [Remarks] 京都新聞2009年6月20日
• [Remarks] 科学新聞2009年7月10日