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Establishment and characterization of novel tumor cell migration factor, TRPV2 channel

Research Project

Project/Area Number 20790247
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionGunma University

Principal Investigator

NAKAGAWA Yuko  Gunma University, 生体調節研究所, 助教 (90422500)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsがん / Ca^<2+>透過性チャネル / チャネル / カルシウム
Research Abstract

The present study was conducted to characterize the regulation and function of TRPV2 channel in highly malignant tumor cell. The expression of TRPV2 was not only detected in the normal cell, but also in the tumor cell. We then determined localization of TRPV2 using metastatic B16 melanoma murine cells transfected with TRPV2-EGFP. In serum-free condition, most of the TRPV2 signal was located in the cytoplasm. Treatment with serum induced translocation of some of the TRPV2-EGFP to the plasma membrane. Serum-induced translocation was blocked by transfection of short-form TRPV2 (s-TRPV2) lacking a pore-forming region and the sixth transmembrane domain. These data conformed to that of macrophage TtT/M87 cell. To determine the function of migration for the TRPV2, we performed the wound healing assay. The ruthenium red, TRPV family inhibitor, blocked the migration of B6 cell. However s-TRPV2 couldn't inhibit the cell migration.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion.2009

    • Author(s)
      Yuko Nakagawa, Itaru Kojima., (他8名, 1番目)
    • Journal Title

      PLos One. 4

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion2009

    • Author(s)
      Nakagawa Y, Nagasawa M, Yamada S, Hara A, Mogami H, Nikolaev VO, Lohse MJ, Shigemura N, Ninomiya Y, Kojima I.
    • Journal Title

      PLos One. 4

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sweet taste receptor expreSsed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion2009

    • Author(s)
      Yuko Nakagawa, Masahiro Nagasawa, Satoko Yamada, Akemi Hara, Hideo Mogami, Viacheslav O. Nikolaev, Martin J. Lohse, Noriatsu Shigemura, Yuzo Ninomiya, and Itaru Kojima
    • Journal Title

      PLoS ONE 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 膵β細胞における甘味感受体T1R2+T1R3の機能解析2009

    • Author(s)
      中川祐子、原朱美、長澤雅裕、最上秀夫、小島至
    • Organizer
      第82回日本内分泌学会学術総会
    • Place of Presentation
      群馬県民会館(群馬県)
    • Year and Date
      2009-04-24
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.imcr.gunma-u.ac.jp/organization/organization5

    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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