Comprehensive analysis of lipid mediators in murine disease models

• Project/Area Number: 20790248
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KITA Yoshihiro The University of Tokyo, 大学院・医学系研究科, 助教 (20401028)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 代謝異常学 / 脂質メタボロミクス / 脂質メディエーター / 質量分析 / 炎症 / エイコサノイド / ホスホリパーゼA2

Research Abstract

Quantitative lipid mediator profiling strategy developed by the investigator was applied to several murine disease models that include inflammatory disease models. Lipid mediator profiles that characterize disease status were successfully determined in murine models of multiple sclerosis, sepsis, etc., which paved the way for a comprehensive analysis of lipid mediators in diseases.

Research Products

• [Journal Article] Prostaglandin F2α receptor signaling facilitates bleomycin-induced pulmonary fibrosis independently of transforming growth factor-β. (2009)
  • Author(s): Oga, T., Matsuoka, T., Yao, C., Nonomura, K., Kitaoka, S., Sakata, D., Kita, Y., Tanizawa, K., Taguchi, Y., Chin, K., Mishima, M., Shimizu, T., Narumiya, S.
  • Journal Title: Nature Med. 12 Pages: 1426-1430
  • Peer Reviewed
• [Journal Article] Targeted lipidomics reveals mPGES-1-PGE2 as a therapeutic target for multiple sclerosis. (2009)
  • Author(s): Kihara, Y., Matsushita, T., Kita, Y., Uematsu, S., Akira, S., Kira, J. -i., Ishii, S., Shimizu, T.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 106 Pages: 21807-21812
  • Peer Reviewed
• [Journal Article] Demethylallosamidin, a chitinase inhibitor, suppresses airway inflammation and hyperresponsiveness. (2009)
  • Author(s): Matsumoto, T., Inoue, H., Sato, Y., Kita, Y., Nakano, T., Noda, N., Eguchi-Tsuda, M., Moriwaki, A., Kan-O, K., Matsumoto, K., Shimizu, T., Nagasawa, H., Sakuda, S., Nakanishi, Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 390 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Targeted lipidomics reveals mPGES-1-PGE2 as a therapeutic target for multiple sclerosis (2009)
  • Author(s): Kihara, Y., Matsushita, T., Kita, Y., Uematsu, S., Akira, S., Kira, J.-i., Ishii, S., Shimizu, T.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 106 Pages: 21807-21812
  • Peer Reviewed
• [Journal Article] Demethylallosamidin, a chitinase inhibitor, suppresses airway inflammation and hyperresponsiveness (2009)
  • Author(s): Matsumoto, T., Inoue, H., Sato, Y., Kita. Y., Nakano, T., Noda, N., Eguchi-Tsuda, M., Moriwaki, A., Kan-O, K., Matsumoto, K., Shimizu, T., Nagasawa, H., Sakuda, S., Nakanishi, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 390 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Prostaglandin F2α receptor signaling facilitates bleomycin-induced pulmonary fibrosis independently of transforming growth factor-β (2009)
  • Author(s): Oga, T., Matsuoka, T., Yao, C., Nonomura, K., Kitaoka, S., Sakata, D., Kita, Y., Tanizawa, K., Taguchi, Y., Chin, K., Mishima, M., Shimizu, T., Narumiya, S.
  • Journal Title: Nature Med. 12 Pages: 1426-1430
  • Peer Reviewed
• [Journal Article] Platelet-Activating Factor Production in the Spinal Cord of Experimental Allergic Encephalomyelitis Mice via the Group IVA Cytosolic PLA2-LysoPAFAT Axis. (2008)
  • Author(s): Kihara, Y., Yanagida, K., Masago, K., Kita, Y., Hishikawa, D., Shindou, H., Ishii, S., Shimizu, T.
  • Journal Title: J. Immunol. 181 Pages: 5008-5014
  • Peer Reviewed
• [Journal Article] Modulation of lipid and protein mediators of inflammation by cytosolic phospholipase A2α during experimental sepsis. (2008)
  • Author(s): Uozumi, N., Kita, Y., Shimizu, T.
  • Journal Title: J. Immunol. 181 Pages: 3558-3566
  • Peer Reviewed
• [Journal Article] Arachidonic acid metabolites inhibit the stimulatory effect of angiotensin II in renal proximal tubules. (2008)
  • Author(s): Li, Y., Yamada, H., Kita, Y., Suzuki, M., Endo, Y., Horita, S., Yamazaki, O., Shimizu, T., Seki, G., Fujita, T.
  • Journal Title: Hypertens. Res. 31 Pages: 2155-2164
  • NAID: 10024828609
  • Peer Reviewed
• [Journal Article] Platelet- Activating Factor Production in the Spinal Cord of Experimental Allergic Encephalomyelitis Mice via the Group IVA Cytosolic PLA2- LysoPAFAT Axis (2008)
  • Author(s): Kihara, Y., Yanagida, K., Masago, K., Kita Y., Hishikawa, D., Shindou, FL, Ishii, S., and Shimizu, T.
  • Journal Title: Journal of Immunology 181 Pages: 5008-5014
  • Peer Reviewed
• [Journal Article] Modulation of lipid and protein mediators of inflammation by cytosolic phospholipase A2α during experimental sepsis (2008)
  • Author(s): Uozumi, N., Kita. Y., and Shimizu, T.
  • Journal Title: Journal of Immunology 181 Pages: 3558-3566
  • Peer Reviewed
• [Journal Article] Arachidonic acid metabolites inhibit the stimulatory effect of angiotensin II in renal proximal tubules (2008)
  • Author(s): Li Y, Yamada H, Kita Y, Suzuki M, Endo Y, Horita S, Yamazaki O, Shimizu T, Seki G, Fujita T.
  • Journal Title: Hypertension Research 31 Pages: 2155-2164
  • NAID: 10024828609
  • Peer Reviewed
• [Presentation] QUANTITATIVE LIPIDOMICS STRATEGY BY LC-ESIMS: METHODOLOGY AND APPLICATION IN BIOLOGICAL STUDIES (2009)
  • Author(s): Yoshihiro Kita, Yasuyuki Kihara, Satoshi Ishii, Keisuke Yoshikawa, Atsuyoshi Shimada, Takao
  • Organizer: 4th International Congress on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 東京(学術総合センター)
  • Year and Date: 2009-05-28
• [Presentation] QUANTITATIVE LIPIDOMICS STRATEGY BY LC-ESIMS : METHODOLOGY AND APPLICATION IN BIOLOGICAL STUDIES (2009)
  • Author(s): Yoshihiro Kita, Yasuyuki Kihara, Satoshi Ishii, Keisuke Yoshikawa, Atsuyoshi Shimada, Takao Shimizu
  • Organizer: 4th International Congress on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 東京(学術総合センター)
  • Year and Date: 2009-05-28
• [Presentation] UPLC-ESIMSによるリン脂質ディファレンシャル解析法の開発および膜リン脂質代謝酵素解析への応用 (2008)
  • Author(s): 北芳博、東知宏、清水孝雄
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] LC-MSによる脂質メディエーターの定量的プロファイリング～ 開発から応用まで (2008)
  • Author(s): 北芳博、清水孝雄
  • Organizer: 第35回BMSコンファレンス(BMS2008)(質量分析学会)
  • Place of Presentation: 裏磐梯ロイヤルホテル(福島県)
  • Year and Date: 2008-07-08
• [Presentation] LC-MSによる脂質メディエーターの定量的プロファイリング〜開発から応用まで (2008)
  • Author(s): 北芳博、清水孝雄
  • Organizer: 第35回BMSコンファレンス (BMS2008) (質量分析学会)
  • Place of Presentation: 裏磐梯ロイヤルホテル (福島県)(口頭発表)
  • Year and Date: 2008-07-08
• [Presentation] UPLC-ESIMS lipidomics strategy for in vitro and in vivo characterization of cytosolic phospholipase A2 enzymes FASEB Summer Research Conferences (2008)
  • Author(s): Kita, Y., Azuma, C., Shimizu, T.
  • Organizer: アメリカ・コネチカット州・ニューヘブン
  • Place of Presentation: ポスター
• [Presentation] UPLC-ESIMS lipidomics strategy for in vitro and in vivo characterization of cytosolic phospholipase A2 enzymes (2008)
  • Author(s): Kita. Y., Azuma, C., and Shimizu, T.
  • Organizer: FASEB Summer Research Conferences
  • Place of Presentation: アメリカ・コネチカット州・ニューヘブン(ポスター発表)
• [Presentation] UPLC-ESIMSによるリン脂質ディファレンシャル解析法の開発および膜リン脂質代謝酵素解析への応用 (2008)
  • Author(s): 北芳博、東知宏、清水孝雄
  • Organizer: BMB2008 (第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会)
  • Place of Presentation: 神戸ポートアイランド(ポスター発表)