Analysis of sulfotransferases involved in biosynthesis of selectin ligand carbohydrates in ulcerative colitis

• Project/Area Number: 20790278
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: Shinshu University
• Principal Investigator: KOBAYASHI Motohiro Shinshu University, 医学系研究科, 講師 (00362137)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 潰瘍性大腸炎 / 翻訳後修飾 / 高内皮細静脈様血管 / MAdCAM-1 / セレクチンリガンド糖鎖 / L-セレクチンリガンド糖鎖

Research Abstract

A diffuse lymphocyte infiltrate characterizes ulcerative colitis, and requires at least one of the two molecular interactions: one via L-selectin and peripheral lymph node addressin (PNAd) and the other via α4β7 integrin and mucosal addressin cell adhesion molecule 1 (MAdCAM-1). The present study was undertaken to elucidate the role of post-translational modification of MAdCAM-1 protein with L-selectin ligand carbohydrates in the pathogenesis of ulcerative colitis. In this study, ulcerative colitis disease activity is not regulated by expression of MAdCAM-1 protein itself, but rather by decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates mediated chiefly by sulfation by GlcNAc6ST-1.

Research Products

• [Journal Article] Tumor suppressor function of laminin-bindingα-dystroglycan requires a distinctβ3-N-acetylglucosaminyltransferase. (2009)
  • Author(s): Bao X, Kobayashi M, Hatakeyama S, Angata K, Gullberg D, Nakayama J, Fukuda MN, Fukuda M
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 12109-12114
  • Peer Reviewed
• [Journal Article] Role of ASC in the mouse model of Helicobacter pylori infection. (2009)
  • Author(s): Benoit NB, Kobayashi M, Kawakubo M, Takeoka M, Sano K, Zou J, Itano N, Tsutsui H, Noda T, Sagara J, Fukuda M, Nakayama J, Taniguchi S
  • Journal Title: J Histochem Cytochem 57 Pages: 327-338
  • Peer Reviewed
• [Journal Article] Role of sulfated O-glycans expressed by HEV-like vessels in pathogenesis of chronic inflammatory gastrointestinal diseases. (2009)
  • Author(s): Kobayashi M, Fukuda M, Nakayama J
  • Journal Title: Biol Pharm Bull 32 Pages: 774-779
  • Peer Reviewed
• [Journal Article] Roles of mucin-type O-glycans in the pathogenesis of Helicobacter pylori infection. (2009)
  • Author(s): Kobayashi M, Lee H, Nakayama J, Fukuda M
  • Journal Title: Glycobiology 19 Pages: 453-461
  • Peer Reviewed
• [Journal Article] Carbohydrate-dependent defense mechanisms against Helicobacter pylori infection. (2009)
  • Author(s): Kobayashi M, Lee H, Nakayama J, Fukuda M
  • Journal Title: Curr Drug Metab 10 Pages: 29-40
  • Peer Reviewed
• [Journal Article] GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis. (2009)
  • Author(s): Kobayashi M, Hoshino H, Masumoto J, Fukushima M, Suzawa K, Kageyama S, Suzuki M, Ohtani H, Fukuda M, Nakayama J
  • Journal Title: Inflamm Bowel Dis 15 Pages: 697-706
  • Peer Reviewed
• [Journal Article] Tumor suppressor function of laminin binding α-dystroglycan requires a distinct β3-N-acetylglucosaminyltransferase (2009)
  • Author(s): Bao X
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 12109-12114
  • Peer Reviewed
• [Journal Article] Role of ASC in the mouse model of Helicobacter pylori infection (2009)
  • Author(s): Benoit NB
  • Journal Title: J Histochem Cytochem 57 Pages: 327-338
  • Peer Reviewed
• [Journal Article] Role of sulfated O-glycans expressed by HEV-like vessels in pathogenesis of chronic inflammatory gastrointestinal diseases (2009)
  • Author(s): Kobayashi M
  • Journal Title: Biol Pharm Bull 32 Pages: 774-779
  • Peer Reviewed
• [Journal Article] Roles of mucin-type O-glycans in the pathogenesis of Helicobacter pylori infection (2009)
  • Author(s): Kobayashi M
  • Journal Title: Glycobiology 19 Pages: 453-461
  • Peer Reviewed
• [Journal Article] Carbohydrate-dependent defense mechanisms against Helicobacter pylori infection (2009)
  • Author(s): Kobayashi M
  • Journal Title: Curr Drug Metabol 10 Pages: 29-40
  • Peer Reviewed
• [Journal Article] GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis (2009)
  • Author(s): Kobayashi M
  • Journal Title: Inflamm Bowel Dis 15 Pages: 697-706
  • Peer Reviewed
• [Presentation] The HECA-452-positive but MECA-79-negative immunohistochemical profile of HEV-like vessels is characteristic for the diagnosis of gastric MALT lymphoma (2009)
  • Author(s): Kobayashi M
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: San Diego, CA,USA
  • Year and Date: 2009-11-13
• [Presentation] Primary colonic signet-ring cell carcinoma presenting carcinocythemia : (2009)
  • Author(s): 小林基弘
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-01
• [Presentation] The HECA-452-positive but MECA-79-negative immunohistochemical profile of HEV-like vessels is characteristic for the diagnosis of gastric MALT lymphoma. (2009)
  • Author(s): Kobayashi M, Mitoma J, Fukuda M, Nakayama J
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Primary colonic signet-ring cell carcinoma presenting carcinocythemia: an autopsy case. (2009)
  • Author(s): 小林基弘, 伊藤誠, 岩谷舞
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
• [Presentation] Decoration of MAdCAM-1 protein with L-selectin ligand carbohydrate mediated by GlcNAc6ST-1 directs disease activity of ulcerative colitis (2008)
  • Author(s): Kobayashi M
  • Organizer: Glycobiology 2008
  • Place of Presentation: Fort Worth, TX, USA
  • Year and Date: 2008-11-13
• [Presentation] Preferential glycosylation of MAdCAM-1 on high endothelial venule-like vessels in ulcerative colitis (2008)
  • Author(s): 小林基弘
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] Decoration of MAdCAM-1 protein with L-selectin ligand carbohydrate mediated by GlcNAc6ST-1 directs disease activity of ulcerative colitis. (2008)
  • Author(s): Kobayashi M, Hoshino H, Masumoto J, Fukushima M, Suzawa K, Fukuda M, Nakayama J
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: Fort Worth, TX, USA
• [Presentation] Laminin-binding glycans attached toα-dystroglycan function as a novel tumor suppressor. (2008)
  • Author(s): Bao X, Kobayashi M, Hatakeyama S, Yu SY, Angata K, Gullberg D, Khoo KH, Nakayama J, Fukuda MN, Fukuda M
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: Fort Worth, TX, USA
• [Presentation] Preferential glycosylation of MAdCAM-1 on high endothelial venule-like vessels in ulcerative colitis. (2008)
  • Author(s): 小林基弘, 福島万奈, 大谷明夫, 中山淳
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
• [Presentation] Preferential induction of PNAd on HEV-like vessels in active phase of ulcerative colitis. (2008)
  • Author(s): 酒井康弘, 小林基弘, 大谷明夫, 中山淳
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
• [Remarks]
  • URL: http://www.shillshu-u.ac.jp/faculty/medicine/chair/i-1byori/default.htm