Analysis of decoy receptors in osteoblastogenesis and osteoclastogenesis : a role in prostate cancer and its bone metastasis

• Project/Area Number: 20790283
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: Kobe University
• Principal Investigator: KONDO Takeshi 神戸大学, 大学院・医学研究科, 講師 (20335441)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: BAMBI / TGF-b / BMP / pseudoreceptor / TGF-β

Research Abstract

In vitro studies revealed that BAMBI mRNA expression significantly increased during osteoblastic differentiation. It was suggested that BAMBI expression plays an important role as part of the negative feedback loop system in TGF-β/BMP signaling and BAMBI is regulated by both TGF-β/BMP and Wnt/β-catenin signaling pathways. I then assessed the methylation status of the BAMBI promoter region and found tissue-specific distribution of promoter hypermethylation. It is therefore speculated that the epigenetic regulation of BAMBI gene expression by CpG hypermethylation is physiologically involved in tissue-and development-specific BAMBI expression. BAMBI is a target gene of both BMP and Wnt/β-catenin signaling and may be involved in preventing some of the osteoblast precursors from terminal differentiation

Research Products

• [Journal Article] Epigenetic Alteration by DNA Promoter Hypermethylation of Genes Related to Transforming Growth Factor-beta (2011)
  • Author(s): Khin SS, Kitazawa R, Kondo T, Idei, Fujimoto M, Haraguchi R, Mori K, Kitazawa S
  • Journal Title: (TGF-beta) Signaling in Cancer Cancers Volume: 3 Pages: 982-993
  • Peer Reviewed
• [Journal Article] Identification and analysis of function of a novel splicing variant of mouse receptor activator of NF-κB (2011)
  • Author(s): Mukai S, Kitazawa R, Ishii J, Kondo T, Hakozaki A, Horiuchi K, Haraguchi R, Mori K, Kitazawa S
  • Journal Title: Mol Cell Biochem Volume: 350 Pages: 29-38
  • Peer Reviewed
• [Journal Article] Identification and analysis of function of a novel splicing variant of mouse receptor activator of□ NF-kappaB (2011)
  • Author(s): Mukai S, et al
  • Journal Title: Mol Cell Biochem Volume: 350 Issue: 1-2 Pages: 29-38
  • DOI: 10.1007/s11010-010-0679-z
  • Peer Reviewed
• [Journal Article] Epigenetic Alteration by DNA Promoter Hypermethylation of Genes Related to Transforming Growth Factor-beta (TGF-beta) Signaling in Cancer (2011)
  • Author(s): Khin SS, et al
  • Journal Title: Cancers Volume: 3 Pages: 982-993
  • Peer Reviewed
• [Journal Article] BAMBI gene is epigenetically silenced in subset of high-grade bladder cancer (2009)
  • Author(s): Khin SS, Kitazawa R, Win N, Aye TT, Mori K, Kondo T, Kitazawa S
  • Journal Title: Int J Cancer Volume: 125 Pages: 328-338
  • Peer Reviewed
• [Journal Article] BAMBI gene is epigenetically silenced in subset of high-grade bladder cancer. (2009)
  • Author(s): Khin SS, Kitazawa R, Win N, Aye TT, Mori K, Kondo T, Kitazawa S.
  • Journal Title: Int J Cancer 125 Pages: 328-338
  • Peer Reviewed
• [Journal Article] BAMBI gene is epigenetically silenced in a subset of high-grade bladder cancer. (2009)
  • Author(s): Khin SS, et al.
  • Journal Title: Int J Cancer 125 Pages: 328-338
  • Peer Reviewed
• [Journal Article] Diffuse cardiac lymphatic involvement by metastatic neuroendocrine carcinoma mimicking hypertrophic cardiomyopathy : a case report. (2009)
  • Author(s): Kondo T, et al.
  • Journal Title: Cases J 2 Pages: 9127-9127
  • Peer Reviewed
• [Journal Article] Modulation of mouse RANKL gene expression by Runx2 and vitamin D3 (2008)
  • Author(s): Kitazawa R, Mori K, Yamaguchi A, Kondo T, Kitazawa S
  • Journal Title: J Cell Biochem Volume: 105 Pages: 1289-1297
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide suppresses RANK gene expression in macrophages by down-regulating PU (2008)
  • Author(s): Ishii J, Kitazawa R, Mori K, McHugh KP, Morii E, Kondo T, Kitazawa S
  • Journal Title: J Cell Biochem Volume: 105 Pages: 896-904
  • Peer Reviewed
• [Journal Article] Gastric remnant adenocarcinoma with micropapillary component (2008)
  • Author(s): Kondo T, Kitazawa R, Kitazawa S
  • Journal Title: Dig Dis Sci Volume: 53 Pages: 2287-2289
  • Peer Reviewed
• [Journal Article] Modulation of mouse RANKL gene expression by Runx2 and vitamin D3. (2008)
  • Author(s): Kitazawa R, Mori K, Yamaguchi A, Kondo T, Kitazawa S.
  • Journal Title: J Cell Biochem 105 Pages: 1289-1297
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide suppresses RANK gene expression in macrophages by down-regulating PU.1 and MITF. (2008)
  • Author(s): Ishii J, Kitazawa R, Mori K, McHugh KP, Morii E, Kondo T, Kitazawa S.
  • Journal Title: J Cell Biochem 105 Pages: 896-904
  • Peer Reviewed
• [Journal Article] Gastric remnant adenocarcinoma with micropapillary component. (2008)
  • Author(s): Kondo T, Kitazawa R, Kitazawa S.
  • Journal Title: Dig Dis Sci 53 Pages: 2287-2289
  • Peer Reviewed
• [Journal Article] Modulation of mouse RANKL gene expression by Runx2 and vitamin D3 (2008)
  • Author(s): Kitazawa, et.al.
  • Journal Title: J Cell Biochem 105 Pages: 1289-1297
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide suppresses RANK gene expression in macrophages by down-regulating PU.1 and MITF (2008)
  • Author(s): Ishii J, et.al.
  • Journal Title: J Cell Biochem 105 Pages: 896-904
  • Peer Reviewed
• [Journal Article] Gastric Remnant Adenocarcinoma with Micropapillary Component (2008)
  • Author(s): Kondo T, et.al.
  • Journal Title: Dig Dis Sci 53 Pages: 2287-2289
  • Peer Reviewed
• [Journal Article] A p. D116G mutation in CREB1 leads to novel multiple malformation syndrome resembling CrebA knockout mouse
  • Author(s): Kitazawa S, Kondo T, Mori K, Yokoyama N, Matsuo M, Kitazawa R
  • Journal Title: Hum Mutat Volume: (in press)
  • Peer Reviewed
• [Journal Article] A p.D116G mutation in CREB1 leads to novel multiple malformation syndrome resembling CrebA knockout mouse
  • Author(s): Kitazawa S, et al
  • Journal Title: Hum Mutat Volume: (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Identification and analysis of function of a novel splicing variant of mouse receptor activator of NF-κB.
  • Author(s): Mukai S, Kitazawa R, Ishii J, Kondo T, Hakozaki A, Horiuchi K, Haraguchi R, Mori K, Kitazawa S.
  • Journal Title: Mol Cell Biochem in press
  • Peer Reviewed
• [Journal Article] Identification and analysis of function of a novel splicing variant of mouse receptor activator of NF-kappaB
  • Author(s): Mukai S, et al.
  • Journal Title: Mol Cell Biochem Volume: (in press)
  • Peer Reviewed
• [Presentation] マウスBAMBI遺伝子発現調節機構の解析 (2009)
  • Author(s): 近藤武史, ほか
  • Organizer: 日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-05-03
• [Presentation] マウスBAMBI遺伝子発現調節機構の解析 (2009)
  • Author(s): 近藤武史, ほか
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
• [Presentation] マウスBAMBI遺伝子発現の調節機構 (2008)
  • Author(s): 近藤武史, ほか
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
• [Presentation] マウスBAMBl遺伝子発現の調節機構 (2008)
  • Author(s): 近藤武史, ほか
  • Organizer: 日本病理学会総会
  • Place of Presentation: 金沢
• [Book] わたしの病気は何ですか?.病理診断科への招待- (2010)
  • Author(s): 近藤武史、榎木英介
  • Total Pages: 96
  • Publisher: 岩波書店
• [Book] 病理診断科への招待(わたしの病気は何ですか?) (2010)
  • Author(s): 近藤武史、榎木英介
  • Total Pages: 96
  • Publisher: 岩波書店
• [Book] わたしの病気は何ですか?-病理診断科への招待- (2010)
  • Author(s): 近藤武史、榎木英介
  • Total Pages: 96
  • Publisher: 岩波書店
• [Remarks] ホームページ
  • URL: http://researchmap.jp/takeshikondo/
• [Remarks] ホームページ
  • URL: http://researchmap.jp/takeshikondo/
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/patho2/
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/patho2/