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A novel diagnostic monoclonal antibody specific for Helicobacter pylori CagA of East Asian type.

Research Project

Project/Area Number 20790285
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionOita University

Principal Investigator

UCHIDA Tomohisa  Oita University, 医学部, 助教 (70381035)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsヘリコバクター・ピロリ / CagA,病原性 / ELISA / モノクローナル抗体 / 胃がん / 胃炎 / H.pylori / cagA遺伝子 / 免疫組織化学 / 胃癌 / 診断法 / DNA解析 / 東アジア型CagA / cahA遺伝子
Research Abstract

We developed and validated an enzyme linked immunosorbent assay (ELISA) using a monoclonal antibody specifically recognizing East Asian CagA positive H. pylori. A total of 32 H. pylori strains were tested and the data were subjected to receiver operator characteristic (ROC) curve analysis. The accuracy of the test, determined by calculating the area under the curve, was 0.96, which indicated a high level of accuracy. At the ROC optimized cut off, the sensitivity and specificity of our ELISA method were 88.0% and 100%, respectively. The validated ELISA showed good performance in terms of sensitivity and specificity. These results suggest that this test is suitable for the diagnostic detection of East Asian CagA carrying strains. We also analyzed the localization of the CagA protein in H. pylori infected gastric mucosa with fluorescence immunohistochemistry, and found that CagA protein expression was up regulated by adhesion to epithelial cells.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (12 results)

All 2010 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (5 results)

  • [Journal Article] Evaluation of Rapid Urine Test for the Detection of Helicobacter pylori Infection in the Vietnamese Population.2010

    • Author(s)
      Nguyen LT, Uchida T, Taukamoto Y, Trinh TD, Ta L, Ho DQ, Matsuhisa T, Uchida M, Takayama A, Hijiya N, Okimoto T, Kodama M, Murakami K, Fujioka T, Moriyama M.
    • Journal Title

      Dig Dis Sci. 55

      Pages: 89-93

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel diagnostic monoclonal antibody specific for Helicobacter pylori CagA of East Asian type2009

    • Author(s)
      Yasuda A, Uchida T, Nguyen LT, Kawazato H, Tanigawa M, Murakami K, Kishida T, Fujioka T, Moriyama M.
    • Journal Title

      Apmis 117

      Pages: 893-9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Helicobacter pylori dupA gene is not associated with clinical outcomes in the Japanese population.2009

    • Author(s)
      Nguyen LT, T, Tsukamoto Y, Kuroda A, Okimoto T, Kodama M, Murakami K, Fujioka T, MM.
    • Journal Title

      Clin Microbiol Infect. (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel diagnostic monoclonal antibody specific for Helicobacter pylori CagA of East Asian type2009

    • Author(s)
      Yasuda A, Uchida T, Nguyen LT, Kawazato H, Tanigawa M, Murakami K, Kishida T, Fujioka T, Moriyama M
    • Journal Title

      APMIS 117

      Pages: 893-899

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Helicobacter pylori virulence and the diversity of gastric cancer in Asia2008

    • Author(s)
      Nguyen LT, Uchida T, Murakami K, Fujioka T, Moriyama M.
    • Journal Title

      Journal of Medical Microbiology 57

      Pages: 1445-1453

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotyping of the cagA gene of Helicobacter pylori on immunohistoche mistry with East Asian CagA-specific antibody.2008

    • Author(s)
      Kanada R, Uchida T, Tsukamoto Y, Nguyen LT, et.al.
    • Journal Title

      Pathol International 58

      Pages: 218-225

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide analysis of DNA copy number alterations and gene expres sion in gastric cancer2008

    • Author(s)
      Tsukamoto Y, Uchida T, Karnan S, et.al.
    • Journal Title

      Journal of Pathology 216

      Pages: 471-482

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Evaluation of the anti-East Asian CagA specific antibody for cagA gene genotyping in Thailand and Vietnam2009

    • Author(s)
      Tomohisa Uchida, et al.
    • Organizer
      日本ヘリコバクター学会
    • Place of Presentation
      東京ステーションコンファレンス
    • Year and Date
      2009-06-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] ベトナムにおける東アジア型Hehcobacter pyloTiの感染頻度2008

    • Author(s)
      内田智久、Nguyell Laln Tung, et.al.
    • Organizer
      第14回日本ヘリコバクター学会
    • Place of Presentation
      神戸市中央区
    • Year and Date
      2008-06-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 東アジア型CagA特異抗体を用いたタイとベトナムにおけるH. pylori cagA遺伝子型の決定

    • Author(s)
      内田智久、沖本忠義、兒玉雅明、松久威史、村上和成、藤岡利生、守山正胤
    • Organizer
      第15回日本ヘリコバクター学会学術集会
    • Related Report
      2009 Final Research Report
  • [Presentation] ヘリコバクター・ピロリが有する東アジア型CagA蛋白質に対するモノクローナル抗体の開発による東アジア型CagAを診断するELISA法の確立

    • Author(s)
      内田智久, Nguyen Lam Tung, 村上和成, 藤岡利生, 守山正胤
    • Organizer
      第16回日本ヘリコバクター学会学術集会
    • Related Report
      2009 Final Research Report
  • [Presentation] Shigetaka Yanagisawa and Masatsugu Moriyama Genotyping of the cagA Gene of Helicobacter pylori by Immunohistochemistry with East Asian CagA-specific Antibody

    • Author(s)
      Tomohisa Uchida, Ryoko Kanada, Yoshiyuki Tsukamoto, Lam Tung Nguyen, Naoki Hijiya, Keiko Matsuura, Masaaki Kodama, Tadayoshi Okimoto, Kazunari Murakami, Toshio Fujioka
    • Organizer
      7th China-Korea-Japan Joint Conference on Helicobacter Infection
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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