Elucidation of oncolytic mechanisms of sindbis virus

• Project/Area Number: 20790297
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Chiba University
• Principal Investigator: SAITO Kengo Chiba University, 大学院・医学研究院, 助教 (70451755)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腫瘍 / シンドビスウイルス / 腫瘍溶解 / 構造蛋白 / 癌 / ウイルス

Research Abstract

I identified sindbis virus (SIN) has remarkable oncolytic effects on human cancer cell lines, and examined the involvement of the major components of SIN structural proteins, capsid, E2, and E1, in the oncolysis. E1 was shown to play an important role in the oncolysis. This finding suggested that the E1 protein may be used as an agent for cancer therapy.

Research Products

• [Journal Article] Oncolytic virotherapy for oral squamous cell carcinoma using replication-competent viruses. (2009)
  • Author(s): Saito K, Shirasawa H, Isegawa N, Shiiba M, Uzawa K, Tanzawa H
  • Journal Title: Oral Oncology 45(12) Pages: 1021-7
  • Peer Reviewed
• [Journal Article] Oncolytic activity of Sindbis virus in human oral squamous carcinoma cells. (2009)
  • Author(s): Saito K, Uzawa K, Kasamatsu A, Shinozuka K, Sakuma K, Yamatoji M, Shiiba M, Shino Y, Shirasawa H, Tanzawa H.
  • Journal Title: Br J Cancer. 101(4) Pages: 684-90
• [Journal Article] Oncolytic virotherapy for oral squamous cell carcinoma using replication-competent viruses (2009)
  • Author(s): 齋藤謙悟、白澤浩、丹沢秀樹, ほか3名
  • Journal Title: Oral Oncology 45 Pages: 1021-27
  • Peer Reviewed
• [Journal Article] Oncolytic activity of Sindbis virus in human oral squamous carcinoma cells (2009)
  • Author(s): 齋藤謙悟、白澤浩、丹沢秀樹, ほか3名
  • Journal Title: British Journal of Cancer 101 Pages: 684-690
  • Peer Reviewed
• [Presentation] The effect of a cisplatin-encapsulating cancer-targeted lipsome with viral proteins on cancer cells. (2009)
  • Author(s): Saito K., Shirasawa H., Uzawa K., Tanzawa H.
  • Organizer: 68th Annual Meeting of the japanese Cancer Association Yokohama
  • Year and Date: 2009-10-03
• [Presentation] The effect of a cisplatin-encapsulating cancer-targeted lipsome with viral proteins on cancer cells (2009)
  • Author(s): 齋藤謙悟
  • Organizer: 第68回 日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] Oncolytic activity of sindbis virus for oral cancer cells (2008)
  • Author(s): Saito k., Shirasawa H., Shiiba M., Uzawa K., Tanzawa H.
  • Organizer: 3th World Congress on Advances in Oncology and 11th International Symposium on Molecular Medicine
  • Place of Presentation: Greece Krete
  • Year and Date: 2008-10-09
• [Presentation] Oncolytic effects of UV-inactivated Sindbis virus in human neuroblastoma cells (2008)
  • Author(s): 八幡江里子
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-09-28
• [Remarks] DNA DATA BANK登録Accession number AB372876 Sindbis virus genomic RNA, 26S messenger RNA region, complete sequence, ov-100 variant.
• [Patent(Industrial Property Rights)] 腫瘍特異性を有する新型リポソーム (2010)
  • Inventor(s): 齋藤謙悟、丹沢秀樹、白澤浩、山本惠司、森部久仁一、鵜沢一弘、椎葉正史
  • Industrial Property Rights Holder: 千葉大学
  • Industrial Property Number: 2010-086071
  • Filing Date: 2010-04-02
• [Patent(Industrial Property Rights)] 腫瘍特異性を有するリポソーム (2010)
  • Inventor(s): 齋藤謙悟
  • Industrial Property Number: 2010-086071
  • Filing Date: 2010-04-02