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Identification of Cancer Stem Cells in Murine Tumors

Research Project

Project/Area Number 20790300
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionGifu University

Principal Investigator

HIRATA Akihiro  Gifu University, 生命科学総合研究支援センター, 助教 (30397327)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsがん幹細胞 / 大腸がん / 組織幹細胞 / β-カテニン / Wntシグナル伝達経路 / Wntシグナル / 発がん / 実験病理学
Research Abstract

The presence of cancer stem cells in murine colon tumors was investigated, focusing on the common characteristics with tissue stem cells. Firstly, we examined the underling mechanisms on the control of cellular differentiation and proliferation in the normal colon epithelium and found that the induction of higher level of β-catenin induced the colonic stem cells dividing slowly. Next, we examined the expression pattern of β-catenin in murine colon tumors and found the cells expressing a higher level of β-catenin and dividing more slowly than surrounding tumor cells, indicating the presence of cancer stem-like cells in murine colon tumors.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (42 results)

All 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (27 results) Remarks (3 results)

  • [Journal Article] Chemopreventive effects of fermented brown rice and rice bran (FBRA) on the inflammation-related colorectal carcinogenesis in Apc^<Min/+> mice.2010

    • Author(s)
      Phutthaphadoong S, Yamada Y, Hirata A, Tomita H, Taguchi A, Hara A, Limtrakul PN, Iwasaki T, Kobayashi H, Mori H.
    • Journal Title

      Oncology Reports. 23(1)

      Pages: 23-59

    • NAID

      120006341941

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of initiation activity of 1, 2-dimethylhydrazine in in vivo medium-term liver initiation assay system using 4-week-old rats without hepatocellular proliferative stimuli during the test chemical treatment period.2010

    • Author(s)
      Asaoka Y, Sakai H, Hirata A, Sasaki J, Goryo M, Miyamoto Y, Yanai T, Masegi T, Okada K.
    • Journal Title

      Journal of Veterinary Medical Sciences. 72(1)

      Pages: 43-53

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemopreventive effects of fermented brown rice and rice bran (FBRA) on the inflammation-related colorectal carcinogenesis in Apc^<Min/+> mice.2010

    • Author(s)
      Phutthaphadoong S, Yamada Y, Hirata A, Tomita H, Taguchi A, Hara A, Limtrakul PN, Iwasaki T, Kobayashi H, Mori H.
    • Journal Title

      Oncology Reports 23

      Pages: 53-59

    • NAID

      120006341941

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of initiation activity of 1,2-dimethylhydrazine in in vivo medium-term liver initiation assay system using 4-week-old rats without hepatocellular proliferative stimuli during the test chemical treatment period.2010

    • Author(s)
      Asaoka Y, Sakai H, Hirata A, Sasaki J, Goryo M, Miyamoto Y, Yanai T, Masegi T, Okada K.
    • Journal Title

      Journa of Veterinary Medical Sciences 72

      Pages: 43-53

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequent development of inflammatory lesions and lymphoid foci in the kidneys of Japanese wild crows (Corvus macrorhynchos and Corvus corone) as a result of the entry of causal agents via the renal portal blood.2010

    • Author(s)
      Hirata A, Yonemaru K, Kubo M, Murakami M, Sakai H, Yanai T, Masegi T.
    • Journal Title

      Journa of Veterinary Medical Sciences 72

      Pages: 327-332

    • NAID

      130000134557

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemopreventive effects of fermented brown rice and rice bran against 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanon e-induced lung tumorigenesis in female A/J mice.2009

    • Author(s)
      Phutthaphadoong S, Yamada Y, Hirata A, Tomita H, Taguchi A, Hara A, Limtrakul PN, Iwasaki T, Kobayashi H, Mori H.
    • Journal Title

      Oncology Reports. 21(2)

      Pages: 321-327

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemopreventive effects of fermented brown rice and rice bran against4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis in female A/J mice2009

    • Author(s)
      Phutthaphadoong Supot
    • Journal Title

      Oncology Report 21

      Pages: 321-327

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 expression and β-catenin activation in gastric carcinogenesis in N-methyl-N-nitrosourea-treated K19-C2mE transgenic mice.2008

    • Author(s)
      Takasu S, Tsukamoto T, Cao X, Toyoda T, Hirata A, Ban H, Yamamoto M, Sakai H, Yanai T, Masegi T, Oshima M, Tatematsu M.
    • Journal Title

      Cancer Science. 99(12)

      Pages: 2356-2364

    • NAID

      10024013824

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Carcinogenic risk of heterocyclic amines in combination-assessment with a liver initiation model.2008

    • Author(s)
      Hirata A, Tsukamoto T, Sakai H, Takasu S, Ban H, Imai T, Totsuka Y, Nishigaki R, Wakabayashi K, Yanai T, Masegi T, Tatematsu M.
    • Journal Title

      Food and Chemical Toxicology. 46(6)

      Pages: 2003-2009

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Further upregulation of β-catenin/Tcf transcription is involved in the development of macroscopic tumors in the colon Apc^<Min/+> mice.2008

    • Author(s)
      Oyama T, Yamada Y, Hata K, Tomita H, Hirata A, Sheng H, Hara A, Aoki H, Kunisada T, Yamashita S, Mori H.
    • Journal Title

      Carcinogenesis. 29(3)

      Pages: 666-672

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Carcinogenic risk of heterocyclic amines in combination - assessment with a liver initiation model2008

    • Author(s)
      平田暁大
    • Journal Title

      Food and Chemical Toxicology 46

      Pages: 2003-2009

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 expression and β-catenin activation in gastric carcinogenesis in N-methyl-N-nitrosourea-treated K19-C2mE transgenic mide2008

    • Author(s)
      高須伸二
    • Journal Title

      Cancer Science 99

      Pages: 2356-2364

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 獣医病理病態学からみた比較腫瘍学への貢献-犬の血管肉腫の研究を例に-2010

    • Author(s)
      酒井洋樹、村上麻美、平田暁大、児玉篤史、村井厚子、米丸加余子、丸尾幸嗣、森崇、星野有希、木村透、柳井徳磨
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 獣医病理病態学からみた比較腫瘍学への貢献 -犬の血管肉腫の研究を例に-2010

    • Author(s)
      酒井洋樹、村上麻美、平田暁大、児玉篤史、村井厚子、米丸加余子、丸尾幸嗣、森崇、星野有希、木村透、柳井徳磨
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNA低メチル化(Global DNA hypomethylation)の胃発がん抑制作用-メチル基転移酵素Dnmt1低発現マウスによる検討-2010

    • Author(s)
      平田暁大、山田泰広、富田弘之、山下聡、牛島俊和、原明
    • Organizer
      第26回日本毒性病理学会
    • Place of Presentation
      金沢
    • Year and Date
      2010-02-03
    • Related Report
      2009 Final Research Report
  • [Presentation] DNA低メチル化(Global DNA hypomethylation)の胃発がん抑制作用-メチル基転移酵素Dnmtl低発現マウスによる検討-2010

    • Author(s)
      平田暁大、山田泰広、富田弘之、山下聡、牛島俊和、原明
    • Organizer
      第26回日本毒性病理学会
    • Place of Presentation
      横浜
    • Year and Date
      2010-02-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dkk-1 heterozygousity dose not promote colon tumor development in Apc (Min/+) mice with Sfrps heterozygousity2009

    • Author(s)
      平田暁大、山田泰広、尾山武、原明
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Role of epigenetic modifications in colon tumorigenesis2009

    • Author(s)
      山田泰広、波多野裕一郎、平田暁大、原明
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Dkk-1 heterozygousity dose not promote colon tumor development in Apc(Min/+) mice with Sfrps heterozygousity..2009

    • Author(s)
      平田暁大、山田泰広、尾山武、原明
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of epigenetic modifications in colon tumorigenesis.2009

    • Author(s)
      山田泰広、波多野裕一郎、平田暁大、原明
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] Global hypomethylation suppresses inflammation-related colon carcinogenesis in mice2009

    • Author(s)
      波多野裕一郎、山田泰広、平田暁大、畑和也、田口綾子、境浩康、馬場政司、原明
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Final Research Report
  • [Presentation] Roles of Bone Marrow Derived Cells in Helicobacter pylori Associated Mouse Gastric Carcinogenesis2009

    • Author(s)
      高須伸二、豊田武士、時亮、平田暁大、山本昌美、田口修、立松正衞、塚本徹哉
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Final Research Report
  • [Presentation] Global hypomethylation suppresses inflammation-related colon carcinogenesis in mice.2009

    • Author(s)
      波多野裕一郎、山田泰広、平田暁大、畑和也、田口綾子、境浩康、馬場政司、原明
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Roles of Bone Marrow Derived Cells in Helicobacter pylori Associated Mouse Gastric Carcinogenesis.2009

    • Author(s)
      高須伸二、豊田武士、時亮、平田暁大、山本昌美、田口修、立松正衞、塚本徹哉
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 4週齢ラットを用いた中期イニシエーション活性検索法の有用性2009

    • Author(s)
      浅岡由次、酒井洋樹、平田暁大、佐々木淳、御領政信、宮本庸平、柳井徳磨、柵木利昭、岡田幸助
    • Organizer
      第36回日本トキシコロジー学会学術年会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-07-06
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] N-bis (2-hydroxypropyl) nitrosamine誘発鼻腔内腫瘍に対するp53ノックアウトマウスの発がん感受性2009

    • Author(s)
      平田暁大、塚本徹哉、高須伸二、山本昌美、立松正衞、相磯成敏、福島昭治
    • Organizer
      第16回日本がん予防学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-16
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨髄移植マウスにおけるHelicobacter感染およびMNU誘発胃癌と骨髄由来細胞の関与2009

    • Author(s)
      高須伸二、塚本徹哉、豊田武士、時亮、平田暁大、山本昌美、立松正衞
    • Organizer
      第16回日本がん予防学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-16
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Cyclooxygenase-2阻害剤EtodolacによるDSS誘発Minマウス大腸腫瘍の抑制効果2009

    • Author(s)
      塚本徹哉、時亮、豊田武士、高須伸二、齋藤典子、齋藤亜弓、山本昌美、平田暁大、立松正衞、田中卓二
    • Organizer
      第16回日本がん予防学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-16
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] N-bis(2-hydroxypropyl)nitrosamine誘発鼻腔内腫瘍に対するp53ノックアウトマウスの発がん感受性2009

    • Author(s)
      平田暁大、塚本徹哉、高須伸二、山本昌美、立松正衞、相磯成敏、福島昭治
    • Organizer
      第16回日本がん予防学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Wnt pathwayによる大腸上皮細胞の増殖制御: β-catenin inducibleマウスによる検討2009

    • Author(s)
      平田暁大、山田泰広、山下聡、牛島俊和、原明
    • Organizer
      第25回日本毒性病理学会
    • Place of Presentation
      浜松
    • Year and Date
      2009-01-27
    • Related Report
      2009 Final Research Report
  • [Presentation] Wnt pathwayによる大腸上皮細胞の増殖制御 : β-catenin inducibleマウスによる検討2009

    • Author(s)
      平田暁大
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      浜松
    • Year and Date
      2009-01-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] The role of DNA methylation in carcinogenesis of the digestive tract2008

    • Author(s)
      山田泰広、平田暁大、富田弘之、山下聡、森秀樹、牛島俊和、原明
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-04
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] The epigenetic transcriptional repression of Wnt antagonist genes in the development of colon tumors in Apc^<Min/+> mice2008

    • Author(s)
      尾山武、山田泰広、平田暁大、安井由美子、金美慧、甲野裕之、原明、杉江茂幸、田中卓二、森秀樹
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-04
    • Related Report
      2009 Final Research Report
  • [Presentation] The epigenetic transcriptional repression of Wnt antagonist genes in the development of colon tumors in Apc^<min/+> mice2008

    • Author(s)
      尾山武
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Role of the canonical Wnt signaling in prolifbration and differentiation of the colonic epithelium cell2008

    • Author(s)
      平田暁大、山田泰広、山下聡、牛島俊和、原明、森秀樹
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-03
    • Related Report
      2009 Final Research Report
  • [Presentation] Beta-catenin accumulation and mutation in gastric cancer in H. pylori in fected and MNU treated K19-C2mE transgenic mice2008

    • Author(s)
      高須伸二、塚本徹哉、曹雪源、豊田武士、時亮、平田暁大、大島正伸、立松正衞
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-03
    • Related Report
      2009 Final Research Report
  • [Presentation] Role of the canonical Wnt signaling in proliferation and differentiation of the colonic epithelium cell2008

    • Author(s)
      平田暁大
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Beta-catenin accumulation and mutation in gastric cancer in H. pylori infected and MNU treated K19-C2mE transgenic mice2008

    • Author(s)
      高須伸二
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] 未分化性の維持と発がん2008

    • Author(s)
      山田泰広、原明、平田暁大、廣瀬善信、ルドルフイェーニッシュ、森秀樹
    • Organizer
      第97回日本病理学会総会
    • Place of Presentation
      金沢
    • Related Report
      2009 Final Research Report
  • [Remarks] 第26回日本毒性病理学会会長賞(優秀賞)受賞演題:「DNA低メチル化(Global DNA hypomethylation)の胃発がん抑制作用-メチル基転移酵素Dnmt1低発現マウスによる検討-」

    • Related Report
      2009 Final Research Report
  • [Remarks] 第25回日本毒性病理学会会長賞(最優秀賞)受賞演題:「Wnt pathwayによる大腸上皮細胞の増殖制御: β-catenin inducibleマウスによる検討」

    • Related Report
      2009 Final Research Report
  • [Remarks] 第26回日本毒性病理学会 会長賞(優秀賞)受賞

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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