Generation of a medaka model for in vivo analysis of tumor cells
| Project/Area Number |
20790313
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
HASEGAWA Sumitaka National Institute of Radiological Sciences, 分子イメージング研究センター, 主任研究員 (60415437)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 疾患モデル動物 / がん / 放射線生物学 / 動物モデル / メダカ / 蛍光イメージング / 微量元素 / PIXE分析法 |
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| Research Abstract |
We generated a medaka model capable of allowing the observation of various cell behaviors of transplanted tumors cells, such as cell proliferation and metastasis, which were visualized easily in vivo. Medaka melanoma cells stably expressing green fluorescence protein were established and transplanted into medaka with same genetic background. We could easily visualize tumor growth and metastasis of the transplanted cells at a single level resolution in vivo. Our medaka model provides an opportunity to visualize in vivo tumor cells "as seen in a culture dish" and would be useful for in vivo tumor cell biology.
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Report
(4 results)
Research Products
(6 results)
