Development of myogenic differentiation for cell therapeutic approach

• Project/Area Number: 20790317
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: KASAHARA Yuko National Center of Neurology and Psychiatry, 遺伝子疾患治療研究部, 外来研究員 (90391911)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞 / 細胞移植 / 骨格筋 / 間葉系幹細胞 / 筋分化 / 筋ジストロフィー / 骨格筋分化誘導因子 / モデル動物

Research Abstract

We could successfully achieve effective myogenic differentiation of mesenchymal stromal cells (MSCs) by MyoD overexpression. This study suggests that MSCs would be a promising therapeutic tool for the treatment of Duchenne muscular dystrophy with allogenic transplantation.

Research Products

• [Journal Article] Cellular vimentin content affects the protein level of Hepatitis C virus core protein and the activity of Hepatitis C virus production in cultured cells. (2009)
  • Author(s): Yuko Nitahara-Kasahara, Masayoshi Fukasawa, Fumiko Shinkai-Ouchi, Shigeko Sato, Tetsuro Suzuki, Kyoko Murakami, Takaji Wakita, Kentaro Hanada, Tatsuo Miyamura, Masahiro Nishijima
  • Journal Title: Virology Vol.383 Pages: 319-327
  • Peer Reviewed
• [Journal Article] Scalable Purification of Adeno-associated Virus Serotype 1 (AAV1) and AAV8 Vectors, Using Dual Ion-Exchange Adsorptive Membranes. (2009)
  • Author(s): Takashi Okada, Mutsuko Nonaka-Sarukawa, Ryosuke Uchibori, Kazue Kinoshita, Hiromi Hayashita-Kinoh, Yuko Nitahara-Kasahara, Shin'ichi Takeda, Keiya Ozawa
  • Journal Title: Human Gene Therapy Vol.20,No.9 Pages: 1013-1021
  • Peer Reviewed
• [Journal Article] Scalable Purification of Adeno-associated Vims Serotype 1 (AAV1) and AAV8 Vectors, Using Dual Ion-Exchange Adsorptive Membranes. (2009)
  • Author(s): Takashi Okada, et. al.
  • Journal Title: Human Gene Therapy 20 Pages: 1013-1021
  • Peer Reviewed
• [Journal Article] Cellular vimentin content affects the protein level of Hepatitis C virus core protein and the activity of Hepatitis C virus productionin cultured cells (2009)
  • Author(s): Yuko Nitahara-Kasahara
  • Journal Title: Virology 383 Pages: 319-327
  • Peer Reviewed
• [Journal Article] ウイルスベクターを用いた筋ジストロフィーの治療法開発 (2008)
  • Author(s): 笠原優子
  • Journal Title: 医学の歩み 226 Pages: 379-383
• [Journal Article] Improvement of ECG abnormalities and cardiac fibrosis in dystrophic mice by rAAV9-mediated microdystrophin transduction.
  • Author(s): Jin-Hong Shin, Hiromi Hayashita-Kinoh, Yuko Nitahara-Kasahara, Takashi Okada, Shin'ichi Takeda
  • Journal Title: (in submission)
• [Presentation] 9型AAVベクターを用いた筋ジストロフィー犬胎児・新生児への遺伝子導入 (2009)
  • Author(s): 武田伸一
  • Organizer: 厚生労働省精神・神経疾患研究委託費筋ジストロフィーに対する治療研究を臨床に展開するための統括的研究会
  • Place of Presentation: 都市センターホテル/東京
  • Year and Date: 2009-12-04
• [Presentation] 9型AAVベクターを用いた筋ジストロフィー犬胎児・新生児への遺伝子導入 (2009)
  • Author(s): 喜納裕美、岡田浩典、笠原優子、岡田尚巳、武田伸一
  • Organizer: 第4回筋ジストロフィー研究合同発表会
  • Place of Presentation: 岡山, 倉敷由加温泉ホテル桃花
  • Year and Date: 2009-10-31
• [Presentation] 9型AAVベクターを用いた筋ジストロフィー犬胎児・新生児への遺伝子導入 (2009)
  • Author(s): 喜納裕美
  • Organizer: 第4回筋ジストロフィー研究合同発表会
  • Place of Presentation: 倉敷由加温泉ホテル桃花/岡山県
  • Year and Date: 2009-10-31
• [Presentation] MyoD発現アデノウイルスベクターによる骨髄間葉系幹細胞の骨格筋分化誘導と細胞移植治療 (2009)
  • Author(s): 笠原(仁田原)優子、喜納(早下)裕美、西山章代、Jin-Hong Shin、大島幸子、岡田尚巳、武田伸一
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京, 都市センターホテル
  • Year and Date: 2009-10-26
• [Presentation] MyoD発現アデノウイルスベクターによる骨髄問葉系幹細胞の骨格筋分化誘導と細胞移植治療 (2009)
  • Author(s): 笠原(仁田原)優子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル/東京都
  • Year and Date: 2009-10-25
• [Presentation] AAVベクターを用いた筋ジストロフィーに対する遺伝子治療 (2009)
  • Author(s): 岡田尚巳, 喜納裕美, 笠原優子, 岡田浩典, 武田伸一
  • Organizer: 日本人類遺伝学会第54回大会
  • Place of Presentation: 東京, グランドプリンスホテル高輪
  • Year and Date: 2009-09-24
• [Presentation] AAVベクターを用いた筋ジストロフィーに対する遺伝子治療 (2009)
  • Author(s): 岡田尚巳
  • Organizer: 日本人類遺伝学会第54回大会
  • Place of Presentation: グランドプリンスホテル高輪/東京都
  • Year and Date: 2009-09-24
• [Presentation] Cell therapeutic approach to duchenne muscular dystrophy using myogenic differentiation of multipotent mesencymal stromal cells in dog (2009)
  • Author(s): Yuko Nitahara-Kasahara, Hiromi Hayashita-Kinoh, Jin-Hong Shin, Akiyo Nishiyama, Sachiko Ohshima-Hosoyama, Michiko Wada-Maeda, Akinori Nakamura, Takashi Okada, Shin'ichi Takeda.
  • Organizer: Japan Society of Gene Therapy The 15th Annual Meeting 2009
  • Place of Presentation: Osaka, 大阪大学
  • Year and Date: 2009-07-11
• [Presentation] Cell therapeutic approach to duchenne muscular dystrophy using myogenic differentiation of multipotent mesencymal stromal cells in dog. (2009)
  • Author(s): Yuko Nitahara-Kasahara
  • Organizer: Japan Society of Gene Therapy Annual Meeting
  • Place of Presentation: 大阪大学/大阪府
  • Year and Date: 2009-07-11
• [Presentation] Myogenic differentiation of multipotent mesencymal stromal cell and cell therapeutic approach to duchenne muscular dystrophy. (2008)
  • Author(s): Yuko Nitahara-Kasahara, Akiyo Nishiyama, Jin-Hong Shin, Sachiko Ohshima-Hosoyama, Takashi Okada, Shin'ichi Takeda.
  • Organizer: Japan Society of Gene Therapy The 15th Annual Meeting
  • Place of Presentation: Sapporo, 札幌医科大学
  • Year and Date: 2008-06-14
• [Presentation] Myogenic differentiation of multipotent mesencymal stromal cell and cell therapeutic approach to duchenne muscular dystrophy (2008)
  • Author(s): Yuko Nitahara-Kasahara
  • Organizer: Japan Society of Gene Therapy Annual Meeting
  • Place of Presentation: Sapporo
  • Year and Date: 2008-06-14
• [Presentation] Improvement of cardiac conduction abnormalities by rAAV-9-mediated microdystrophin transduction in mdx. (2008)
  • Author(s): Jin-Hong Shin, Sachiko Ohshima-Hosoyama, Yuko Nitahara-Kasahara, Takashi Okada, Shin'ichi Takeda.
  • Organizer: Japan Society of Gene Therapy The 15th Annual Meeting
  • Place of Presentation: Sapporo, 札幌医科大学
  • Year and Date: 2008-06-13
• [Presentation] Transduction efficiency and immune response with rAAV8 in dog slkeletal muscle (2008)
  • Author(s): Sachiko Ohshima-Hosoyama
  • Organizer: Japan Society of Gene Therapy Annual Meeting
  • Place of Presentation: Sapporo
  • Year and Date: 2008-06-13
• [Presentation] Effective transduction of Dystrophic dogs with rAAV Serotype 8. (2008)
  • Author(s): Sachiko Ohshima-Hosoyama, Jin-Hong Shin, Akiyo Nishiyama, Yuasa Katsutoshi, Yuko Nitahara-Kasahara, Takashi Okada, Shin'ichi Takeda
  • Organizer: The 11th Annual Meeting, American Society of Gene Therapy
  • Place of Presentation: Boston, MA, USA, John B. Hynes Convention Center
  • Year and Date: 2008-06-01
• [Presentation] Electrocardiographic improvemet of mdx heart by transduction with rAAV9-microdystrophin (2008)
  • Author(s): Jin-Hong Shin
  • Organizer: American Society of Gene Therapy Annual Meeting
  • Place of Presentation: Boston, U.S.A.
  • Year and Date: 2008-06-01
• [Book] 医学の歩み(ウイルスベクターを用いた筋ジストロフィーの治療法開発, Vol.226, No.5) (2008)
  • Author(s): 笠原優子、岡田尚巳、武田伸一