Studies on molecular factors responsible for active growth of Echinococcus multilocularis metacestodes

• Project/Area Number: 20790321
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nihon University
• Principal Investigator: MATUMOTO Jun Nihon University, 生物資源科学部, 講師 (70296169)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 人獣共通寄生虫 / エキノコックス / 寄生虫学 / 発生・分化 / 獣医学 / 寄生虫

Research Abstract

This study aims to elucidate molecular factors responsible for the active growth of Echinococcus multilocularis metacestodes. We established a full-length cDNA library of the parasite by V-capping method. In silico analyses of this cDNA library containing over 20,000 clones revealed expression profile in the larval parasite. We are presently developing a E. multilocularis DNA microarray, which is expected to facilitate comprehensive expression analyses of the parasite. Experimental infections of larval E. multilocularis to 4 inbred mouse strainsidentified DBA/2 and C57BL/10 strains as highly susceptible and resistant strains, respectively. Based on these findings in this study, we are performing further genetic experiments using backcrossing mice with various genetic backgrounds originated from the resistant and susceptible mouse strains.

Research Products

• [Journal Article] Echinococcus multilocularis: Two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs. (2010)
  • Author(s): Kouguchi, H., Matsumoto, J., Katoh, Y., Suzuki, T., Oku, Y., Yagi, K.
  • Journal Title: Experimental Parasitology 124(2) Pages: 238-243
• [Journal Article] Echinococcus multilocularis : Two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs (2010)
  • Author(s): Kouguchi, H., Matsumoto, J., Katoh, Y., 他3名
  • Journal Title: Experimental Parasitology 124 Pages: 238-243
  • Peer Reviewed
• [Journal Article] Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. (2009)
  • Author(s): Dang, Z., Yagi, K., Oku, Y., Kouguchi, H., Kajino, K., Watanabe, J., Matsumoto, J., Nakao, R., Wakaguri, H., Toyoda, A., Sugimoto, C.
  • Journal Title: Vaccine 27(52) Pages: 7339-7345
  • NAID: 120001821839
• [Journal Article] Molecular cloning and characterization of a T24-like protein in Echinococcus multilocularis. (2009)
  • Author(s): Dang, Z., Watanabe, J., Kajino, K., Oku, Y., Matsumoto, J., Yagi, K., Kouguchi, H., Sugimoto, C.
  • Journal Title: Molecular and Biochemical Parasitology 168(1) Pages: 117-119
• [Journal Article] Cutaneous leishmaniasis caused by Leishmania (L. ) major infection in Sindh province, Pakistan. (2009)
  • Author(s): Bhutto, A.M., Soomro, F.R., Baloch, J.H., Matsumoto, J., Uezato, H., Hashiguchi, Y., Katakura, K.
  • Journal Title: Acta Tropica 111(3) Pages: 295-298
• [Journal Article] Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis (2009)
  • Author(s): Dang, Z,, Yagi, K., Oku, Y, 他8名
  • Journal Title: Vaccine 27 Pages: 7339-7345
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of a T24-like protein in Echinococcus multilocularis (2009)
  • Author(s): Dang, Z., Watanabe, J., Kajino, K., 他5名
  • Journal Title: Molecular and Biochemical Parasitology 168 Pages: 117-119
  • Peer Reviewed
• [Presentation] 多包虫のcDNAライブラリーにみられた抗酸化活性関連遺伝子 (2009)
  • Author(s): 平松濡、水上智秋、松本淳、野中成晃、他(計9名)
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 法政大学(東京)
  • Year and Date: 2009-03-28
• [Presentation] 多包虫のcDNAライブラリーに見られた抗酸化活性関連遺伝子. (2009)
  • Author(s): 平松澪、水上智秋、松本淳、野中成晃、片倉賢、李爽、山下理宇、渡辺純一、奥祐三郎
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京