| Project/Area Number |
20790322
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Gunma University |
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Principal Investigator |
HATABU Toshimitsu Gunma University, 医学部, 助教 (60344917)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | マラリア / 貧血 / スカベンジャーレセプター / パターン認識分子 |
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| Research Abstract |
Severe falciparum malaria such as cerebral malaria and severe anemia is leading cause of morbidity and mortality. Plasmodium falciparum-infected red blood cells (pRBCs) adhere to the endothelial cells via receptors expressed on the surface of the endothelial cells, sequester in the microvasculature of several organs. Severe anemia which may be due to a number of factors including rupture of the pRBCs and phagocytosis of pRBCs is another cause of death. However, the molecular mechanism underlying both the cytoadherence and erythrophagocytosis related with severe malaria is not completely understood. Here, we report that the pRBCs bind to the class A scavenger receptors, SR-A and MARCO, which is expressed on the surface of the activated phagocytes.
To identify the cytoadherence of pRBCs to SR-A and MARCO, human SR-A or MARCO cDNA was transfected to CHO cells (CHO-SR-A or CHO-MARCO cells). Furthermore, several mutants of both molecules were made to identify the sites related with pRBC adherence. pRBCs adhered to the both CHO- SR-A and CHO-MARCO cells, but not to the CHO-mock cells. The pRBC did not observed adherence to the deletion mutants of SRCR domain in both scavenger receptors.
These results may suggest that both SR-A and MARCO acts as a host factor related with cytoadherence of the pRBCs, and contribute to our present understanding the pathology of severe falciparum malaria.
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