Investigation of the cytolytic mechanisms of Panton-Valentine leukocidin

• Project/Area Number: 20790331
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Niigata University
• Principal Investigator: NISHIYAMA Akihito Niigata University, 医歯学系, 助教 (80452069)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 病原性 / 市中感染型MRSA / 深部感染 / Panton-Valentineロイコシジン / 毒素活性 / 細菌 / 感染症 / 市中感型MRSA

Research Abstract

Panton-Valentine leukocidin (PVL) is a virulence factor of community-acquired methicillin-resistant Staphylococcus aureus. We have demonstrated the lipid raft-mediated pore formation of PVL. Since integrity of lipid rafts did not affect on the recognition of target cells by PVL, novel PVL receptor(s), other than ganglioside GM1, is suggested. This receptor molecule may be a possible target of PVL inhibitor.

Research Products

• [Journal Article] Community-acquired methicillin-resistant Staphylococcus aureus: community transmission, pathogenesis, and drug resistance (2010)
  • Author(s): Yamamoto T, Nishiyama A, Takano T, Yabe S, Higuchi W, Razvina O, Shi D
  • Journal Title: J. Infect. Chemother (In press. (Invited review))
  • NAID: 10027190012
• [Journal Article] Molecular typing of Campylobacter jejuni and C. coli from chickens and patients with gastritis or Guillain-Barre syndrome based on multilocus sequence types and pulsed-field gel electrophoresis patterns (2010)
  • Author(s): Yabe S, Higuchi W, Iwao Y, Takano T, Razvina O, Reva I, Nishiyama A, Yamamoto T
  • Journal Title: Microbiol. Immunol (In press)
  • NAID: 10029545254
  • Peer Reviewed
• [Journal Article] Emergence of the community-acquired methicillin-resistant Staphylococcus aureus USA300 clone in a Japanese child, demonstrating multiple divergent strains in Japan (2010)
  • Author(s): Higuchi W, Mimura S, Kurosawa Y, Takano T, Iwao Y, Yabe S, Razvina O, Nishiyama A, Ikeda-Dantsuji Y, Sakai F, Hanaki H, Yamamoto T
  • Journal Title: J. Infect. Chemother (In press)
  • NAID: 10027190405
  • Peer Reviewed
• [Journal Article] ブドウ球菌毒素 (2010)
  • Author(s): 西山晃史、高野智洋、樋口渉、山本達男
  • Journal Title: 感染制御 Vol.6No.1 Pages: 33-38
• [Journal Article] Community-acquired methicillin-resistant Staphylococcus aureus : community transmission, pathogenesis, and drug resistance (review) (2010)
  • Author(s): Tatsuo Yamamoto
  • Journal Title: Journal of Infection and Chemotherapy (印刷中)
  • Peer Reviewed
• [Journal Article] ブドウ球菌毒素 (2010)
  • Author(s): 西山晃史
  • Journal Title: 感染制御(総説) 6 Pages: 33-38
• [Journal Article] Emergence of the community-acquired methicillin-resistant Staphylococcus aureus USA300 clone in a Japanese child, demonstrating multiple divergent strains in Japan (2010)
  • Author(s): Wataru Higuchi
  • Journal Title: Journal of Infection and Chemotherapy (印刷中)
  • Peer Reviewed
• [Journal Article] Molecular typing of Campylobacter jejuni and C.coli from chickens and patients with gastritis or Guillain-Barre syndrome based on multilocus seqyuence types and pulsed-field gel electrophoresis patterns.Microbiol (2010)
  • Author(s): Shizuka Yabe
  • Journal Title: Microbiology and Immunology (印刷中)
  • Peer Reviewed
• [Journal Article] Campylobacter jejuniとC.coliのST型別-グローバル分子疫学解析- (2009)
  • Author(s): 矢部静、岩尾泰久、高野智洋、西山晃史、山本達男
  • Journal Title: 日本カンピロバクター研究会誌 Vol.2 Pages: 60-66
• [Journal Article] Streptococcus pneumoniae and Haemophilus influenzae at the initial stage of influenza (2009)
  • Author(s): Takano M, Ozaki K, Nitahara Y, Higuchi W, Takano T, Nishiyama A, Yamamoto T
  • Journal Title: Pediatr. Int 51 Pages: 687-695
  • NAID: 10025861162
  • Peer Reviewed
• [Journal Article] Genotypes, intrafamilial transmission, and virulence potential of nasal methicillin-resistant Staphylococcus aureus from children in the community (2009)
  • Author(s): Ozaki K, Takano M, Higuchi W, Takano T, Yabe S, Nitahara Y, Nishiyama A, Yamamoto T
  • Journal Title: J. Infect. Chemother 15 Pages: 84-91
  • NAID: 10025128460
  • Peer Reviewed
• [Journal Article] A rapidscreening method for Panton-Valentineleucocidin-positive community-acquiredmethicillin-resistant Staphylococcus aureusbelonging to multilocus sequence type 30 andits related clone using a combination ofmultiplex PCR and pulsed-field gelelectrophoresis. (2009)
  • Author(s): Reva I, Higuchi W, Takano T, Singur O, Ozaki K, Isobe H, Yabe S, Saito K, Baranovich T, Enany S, Otsuka T, Potapov V, Nishiyama A, Yamamoto T
  • Journal Title: J. Infect. Chemother 15 Pages: 75-83
  • Peer Reviewed
• [Journal Article] CA-MRSA(PVL産生株、USA300) (2009)
  • Author(s): 山本達男、西山晃史、高野智洋、樋口渉
  • Journal Title: 臨床と微生物 Vol.36No.2 Pages: 103-108
• [Journal Article] Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guerin by intelectin-1 deposited on cell surfaces (2009)
  • Author(s): Tsuji S, Yamashita M, Hoffman DR, Nishiyama A, Shinohara T, Ohtsu T, Shibata Y
  • Journal Title: Glycobiology 19 Pages: 518-526
  • Peer Reviewed
• [Journal Article] Streptococcus pneumoniae and Haemophilus influenzae at the initial stage of influenza (2009)
  • Author(s): Misao Takano
  • Journal Title: Pediatrics International 51 Pages: 687-695
  • Peer Reviewed
• [Journal Article] A rapid screening method for Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type 30 and its related clone using a combination of multiplex PCR and pulsed-field gel electrophoresis (2009)
  • Author(s): Reva I,Nishiyama A,Yamamoto T, 他11名
  • Journal Title: Journal of Infection and Chemotherapy (In press)
  • NAID: 10025128410
  • Peer Reviewed
• [Journal Article] Genotypes, intrafamilial transmission, and virulence potential of nasal methicillin-resistant Staphylococcus aureus from children in the community (2009)
  • Author(s): Ozaki K, Nishiyama A, Yamamoto T, 他5名
  • Journal Title: Journal of Infection and Chemotherapy (In press)
  • NAID: 10025128460
  • Peer Reviewed
• [Journal Article] CA・MRSA(PVL産生株、USA300)(総説) (2009)
  • Author(s): 山本達男、西山晃史、高野智洋、樋口渉
  • Journal Title: 臨床と微生物 36 Pages: 103-108
• [Journal Article] Campylobacter jejuniの構造 (2008)
  • Author(s): 山本達男、西山晃史、矢部静、樋口渉
  • Journal Title: 日本カンピロバクター研究会誌 Vol.1 Pages: 23-27
• [Journal Article] Depletion of cellular cholesterol enhances macrophage MAPK activation by chitin microparticles but not by heat-killed Mycobacterium bovis BCG (2008)
  • Author(s): Nishiyama A, Shinohara T, Pantuso T, Tsuji S, Yamashita M, Shinohara S, Myrvik QN, Henriksen RA, Shibata Y
  • Journal Title: Am. J. Physiol. Cell. Physiol 295
  • Peer Reviewed
• [Presentation] 胃癌関連Helicobacter pylori cagPAIが示す特異構造 (2010)
  • Author(s): 樋口渉、Ivan Reva、Olga Razvina、西山晃史、高野智洋、山本達男
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-06
• [Presentation] わが国の食中毒起因菌Campylobacter jejuni/coliに関する分子疫学調査 (2010)
  • Author(s): Olga Razvina、矢部静、岩尾泰久、樋口渉、高野智洋、西山晃史、山本達男
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-06
• [Presentation] 交通機関、公共施設、大学生に於けるメチシリン耐性ブドウ球菌(MRSA、MRCNS)の分布調査 (2010)
  • Author(s): 矢部静、樋口渉、岩尾泰久、磯辺浩和、高野智洋、西山晃史、山本達男
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-05
• [Presentation] 家族・親族を感染源としたPVL陽性市中感染型MRSAの高頻度再感染の実態と予防対策 (2010)
  • Author(s): 高野智洋、矢部静、樋口渉、岩尾泰久、西山晃史、山本達男
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-05
• [Presentation] Multilocus sequence typing of Campylobacter jejuni and C.coli -Global molecular epidermiology- (2009)
  • Author(s): Yabe S, Iwao Y, Higuchi W, Takano T, Nishiyama A, Razvina O, Yamamoto T
  • Organizer: Colera & Other Bacterial Enteric Infections, 44th Annual Joint Panel Meeting of United States-Japan Cooperative Medical Science Program
  • Place of Presentation: San Diego, CA, U.S.A.
  • Year and Date: 2009-10-13
• [Presentation] Multilocus sequence typing of Campylobacter jejuni and C.coli (2009)
  • Author(s): Shizuka Yabe
  • Organizer: 44^<th> Annual Joint Panel Meeting of United States-Japan Cooper ative Medical Science Program
  • Place of Presentation: San Diego(米国)
  • Year and Date: 2009-10-13
• [Presentation] Unique adherence characteristics of Panton-Valentine leucocidin-positive ST22 methicillin-resistant Staphylococcus aureus from recurrent and/or multifocal cutaneous abscesses (2009)
  • Author(s): Yamamoto T, Yabe S, Shi D, Higuchi W, Iwao Y, Isobe H, Takano T, Nishiyama A
  • Organizer: Abstract (# L1-1672) presented at 49th ICAAC
  • Place of Presentation: San Francisco, CA, U.S.A.
  • Year and Date: 2009-09-15
• [Presentation] Current status of methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci carriage in medical students and the transmission backgrounds in Japan (2009)
  • Author(s): Yabe S, Higuchi W, Iwao Y, Shi D, Takano T, Nishiyama A, Yamamoto T
  • Organizer: Abstract presented at 49th ICAAC
  • Place of Presentation: San Francisco, CA, U.S.A.
  • Year and Date: 2009-09-15
• [Presentation] Unique adherence characteristics of Panton-Valentine leucocidin-positive ST22 methicillin-resistant Staphylococcus aureus from recurrent and/or multifocal cutaneous abscesses (2009)
  • Author(s): Tatsuo Yamamoto
  • Organizer: 49^<th> ICAAC
  • Place of Presentation: San Francisco(米国)
  • Year and Date: 2009-09-14
• [Presentation] Current status of methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci carriage in medical students and the transmission backgrounds in Japan (2009)
  • Author(s): Shizuka Yabe
  • Organizer: 49^<th> ICAAC
  • Place of Presentation: San Francisco(米国)
  • Year and Date: 2009-09-14
• [Presentation] Two types of methicillin-resistant Staphylococcus aureus isolated alone or in combination with methicillin-susceptible S. aureus from bullous impetigo in Japan (2009)
  • Author(s): Shi D, Higuchi W, Iwao Y, Saito K, Yabe S, Takano T, Nishiyama A, Yamamoto T
  • Organizer: Abstract (# C2-121) presented at 49th ICAAC
  • Place of Presentation: San Francisco, CA, U.S.A.
  • Year and Date: 2009-09-12
• [Presentation] Two types of methicillin-resistant Staphylococcus aureus isolated alone or in combination with methicillin-susceptible S.aureus from bullous impetigo in Japan (2009)
  • Author(s): Da Shi
  • Organizer: 49^<th> ICAAC
  • Place of Presentation: San Francisco(米国)
  • Year and Date: 2009-09-12
• [Presentation] otent Anti-Campylobacter jejuni Activity of Carbapenems and the Mode of Action (2009)
  • Author(s): Nishiyama A, Yabe S, Higuchi W, Takano T, Yamamoto T
  • Organizer: Abstract (P-150) presented at 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2009-09-04
• [Presentation] Potent ability of sitafloxacin (fluoroquinolone) as an anti-Helicobacter pylori agent (2009)
  • Author(s): Takano T, Higuchi W, Nishiyama A, Yoshida K, Kanda H, Yamamoto T
  • Organizer: Abstract (P-172) presented at 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2009-09-04
• [Presentation] Types of the cagA gene sequence, especially its tyrosine phosphorylation motif, of Helicobacter pylori strains from Russia (2009)
  • Author(s): Higuchi W, Takano T, Nishiyama A, Reva I, Potapov V, Yamamoto T
  • Organizer: Abstract (P-136) presented at 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2009-09-04
• [Presentation] Molecular epidermiology of Helicobacter pylori isolated from Russia (2009)
  • Author(s): Reva I, Pererva O, Yabe S, Iwao Y, Higuchi W, Takano T, Nishiyama A, Taneike I, Nakagawa S, Potapov V, Yamamoto T
  • Organizer: Abstract (O11-5) presented at 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2009-09-04
• [Presentation] Potent Anti-Campylobacter jejuni Activity of Carbapenems and the Mode of Action (2009)
  • Author(s): Akihito Nishiyama
  • Organizer: 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-04
• [Presentation] Potent ability of sitafloxacin (fluotoquinolone) as an anti-Helicobacter pylori agent (2009)
  • Author(s): Tomomi Takano
  • Organizer: 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-04
• [Presentation] Types of the cagA gene sequence, especially its tyrosine phosphorylation motif, of Helicobacter pylori strains from Russia (2009)
  • Author(s): Wataru Higuchi
  • Organizer: 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-04
• [Presentation] Molecular epidermiology of Helicobacter pylori isolated from Russia (2009)
  • Author(s): Ivan Reva
  • Organizer: 15th International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO2009)
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-04
• [Presentation] Campylobacter jejuniとC. coliのST型別-グローバル分子疫学解析-(シンポジウム) (2009)
  • Author(s): 矢部静、岩尾泰久、高野智洋、西山晃史、山本達男
  • Organizer: 第2回日本カンピロバクター研究会総会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-02
• [Presentation] わが国で分離されたCampylobacter jejuniのST型解析 (2009)
  • Author(s): 岩尾泰久、矢部静、高野智洋、西山晃史、山本達男
  • Organizer: 第2回日本カンピロバクター研究会総会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-02
• [Presentation] ホスホマイシンの新しい作用の探索 (2009)
  • Author(s): 西山晃史、近幸吉、田邊嘉也、下条文武、山本達男
  • Organizer: 第47回新潟化学療法研究会
  • Place of Presentation: 新潟
  • Year and Date: 2009-06-21
• [Presentation] カンピロバクター・ジェジュニの運動性と形態変化 (2008)
  • Author(s): 矢部静、樋口渉、レワ・イワン、高野智洋、西山晃史、山本達男
  • Organizer: 第1回日本カンピロバクター研究会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-02
• [Book] 「市中感染型MRSA」、河野茂 編、「MRSA ?基礎・臨床・対策-」 (2010)
  • Author(s): 西山晃史、高野智洋、樋口渉、山本達男 著
  • Publisher: 医薬ジャーナル社(印刷中)
• [Book] 「市中感染型MRSA」、河野茂 編、「MRSA-基礎・臨床・対策-」 (2010)
  • Author(s): 西山晃史
  • Publisher: 医薬ジャーナル社(印刷中)