Clinical and basic analysis of biosynthetic genes for staphylococcal signaling substances for virulence

• Project/Area Number: 20790332
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Nagoya University
• Principal Investigator: KEIKO Yamada Nagoya University, 大学院・医学系研究科, 助教 (00402561)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: セカンドメッセンジャー / 情報伝達シグナル物質 / cyclic-di-GMP / cyclic-di-AMP / バイオフィルム / カテーテル感染 / ブドウ球菌 / 黄色ブドウ球菌

Research Abstract

Cyclic-di-GMP affected suppressively on biofilm formation. A biosynthetic gene of cyclic-di-AMP became identified and was confirmed its function. It is essential in Staphylococcus aureus. Internal concentration of cyclic-di-GMP and cyclic-di-AMP did not affected by plasmid-mediated overexpression, presumed the strict regulation system on the production of these molecules. Amount of biofilm formation of clinical isolates associated with catheter infection varied among strains and not related with their origins. Biofilm of some strains were affected by DNase, Proteinase K or Dispersin B indicated that components of biofilm also differ among strains.

Research Products

• [Journal Article] Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients. (2010)
  • Author(s): Hasegawa, T., A. Okamoto, T. Kamimura, I. Tatsuno, S.N. Hashikawa, M. Yabutani, M. Matsumoto, K. Yamada, M. Isaka, M. Minami, M. Ohta.
  • Journal Title: Apmis 118 Pages: 167-178
  • Peer Reviewed
• [Journal Article] Evaluation of selection media for the detection of borderline MRSA. (2010)
  • Author(s): Yamada, K., K. Inuzuka, N. Tatsumi, I. Sanzen, T. Ohkura, A. Okamoto, T. Hasegawa, M. Ohta
  • Journal Title: J Infect Chemother 16 Pages: 19-24
  • NAID: 10027189182
  • Peer Reviewed
• [Journal Article] Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients. (2010)
  • Author(s): Hasegawa, T., A.Okamoto, T.Kamimura, I.Tatsuno, S.N.Hashikawa, M.Yabutani, M.Matsumoto, K.Yamada, M.Isaka, M.Minami, M.Ohta
  • Journal Title: Apmis (Acta Pathologica, Microbiologica et Immunologica Scandinavica) 118 Pages: 167-178
  • Peer Reviewed
• [Journal Article] Evaluation of selection media for the detection of borderline MRSA. (2010)
  • Author(s): Yamada, K., K.Inuzuka, N.Tatsumi, I.Sanzen, T.Ohkura, A.Okamoto, T.Hasegawa, M.Ohta
  • Journal Title: J Infect Chemother 16 Pages: 19-24
  • NAID: 10027189182
  • Peer Reviewed
• [Journal Article] Effect of cyclic bis(3'-5')diguanylic acid and its analogs on bacterial biofilm formation (2009)
  • Author(s): Ishihara Y, Hyodo M, Hayakawa Y, Kamegaya T, Yamada K, Okamoto A, Hasegawa T, Ohta M.
  • Journal Title: FEMS Microbiol Lett. 301(2) Pages: 193-200
  • Peer Reviewed
• [Journal Article] Nationwide epidemiological study revealed the dissemination of meticillin-resistant Staphylococcus aureus carrying a specific set of virulence-associated genes in Japanese hospitals. (2009)
  • Author(s): Ohkura, T., K. Yamada, A. Okamoto, H. Baba, Y. Ike, Y. Arakawa, T. Hasegawa, M. Ohta.
  • Journal Title: Journal of Medical Microbiology 58 Pages: 1329-1336
  • Peer Reviewed
• [Journal Article] Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator (agr) locus. (2009)
  • Author(s): Nagao, M., A. Okamoto, K. Yamada, T. Hasegawa, Y. Hasegawa, M. Ohta.
  • Journal Title: BMC microbiology 9 Pages: 52-52
  • Peer Reviewed
• [Journal Article] Effect of cyclic bis(3'-5')diguanylic acid and its analogs on bacterial biofilm formation. (2009)
  • Author(s): Ishihara Y, Hyodo M, Hayakawa Y, Kamegaya T, Yamada K, Okamoto A, Hasegawa T, Ohta M.
  • Journal Title: FEMS Microbiol Lett. 301 Pages: 193-200
  • Peer Reviewed
• [Journal Article] Nationwide epidemiological study revealed the dissemination of meticillin-resistant Staphylococcus aureus carrying a specific set of virulence-associated genes in Japanese hospitals. (2009)
  • Author(s): Ohkura, T., K.Yamada, A.Okamoto, H.Baba, Y.Ike, Y.Arakawa, T.Hasegawa, M.Ohta
  • Journal Title: Journal of Medical Microbiology 58 Pages: 1329-1336
  • Peer Reviewed
• [Journal Article] 病原細菌プロテアーゼの基質探索ストラテジー:SP1Aを例に (2009)
  • Author(s): 山田景子
  • Journal Title: ファルマシア 45 Pages: 1019-1020
  • Peer Reviewed
• [Journal Article] Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator (agr) locus (2009)
  • Author(s): M. Nagao, A. Okamoto, K. Yamada, T. Hasegawa, Y. Hasegawa and M. Ohta
  • Journal Title: BMC Microbiology 9(in press)
  • Peer Reviewed
• [Journal Article] Microchip electrophoresis for specific gene detection of the pathogenic bacteria V. cholerae by circle-to-circle amplification. (2008)
  • Author(s): Mahmoudian, L., J. Melin, M.R. Mohamadi, K. Yamada, M. Ohta, N. Kaji, M. Tokeshi, M. Nilsson, Y. Baba.
  • Journal Title: Anal Sci 24 Pages: 327-332
  • NAID: 10021098849
  • Peer Reviewed
• [Presentation] 黄色ブドウ球菌とセカンドメッセンジャーについて (2009)
  • Author(s): 山田景子(外2名2番目)
  • Organizer: 日本細菌学会中部支部会総会
  • Place of Presentation: 名古屋
• [Presentation] Staphylococcus aureusのバイオフィルム形成におけるc-di-GMPの関与 (2008)
  • Author(s): 山田景子(外3名3番目)
  • Organizer: 日本細菌学会中部支部総会
  • Place of Presentation: 金沢