Functional analysis of novel protein secretion system, T6SS in Vibrio parahaemolyticus

• Project/Area Number: 20790335
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: AKEDA Yukihro Osaka University, 微生物病研究所, 特任講師 (60444527)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腸炎ビブリオ / タンパク質分泌機構 / 6型タンパク質分泌装置

Research Abstract

There are various protein secretion systems in pathogenic gram-negative bacteria. Recently, a novel protein secretion system, T6SS, has been reported. In this project, it has been found that V. parahaemolyticus has a T6SS. which can secrete and translocate a novel protein in T6SS-dependent manner. This protein secretion requires N-terminal region of a novel secreted protein.

Research Products

• [Journal Article] Tetsuya Iida and Takeshi Honda. Aeromonas hydrophila PepO outer membrane endopeptidase activates human big endothelin-3 in vitro and induces skin ulcer in goldfish (Carassius auratus) (2010)
  • Author(s): Said Kamal Abolghait, Yukihiro Akeda, Toshio Kodama, Vlademir V. Cantarelli
  • Journal Title: Veterinary Microbiology (in press)
  • Peer Reviewed
• [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region (2010)
  • Author(s): Toshio Kodama, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Mikiharu Morita, Kaori Izutsu, Yukihiro Akeda, Kwon-Sam Park, Vlademir Cantarelli, Rikard Dryselius, Tetsuya Iida, Takeshi Honda
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enter otoxicity by controlling the expression of genes in the Vp-PAI region. (2010)
  • Author(s): T Kodama, K Gotoh, H Hiyoshi, M Morita, K Izutsu, Y Akeda, KS Park, V Cantarelli, R Dryselius, T lida, T Honda
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] Identification and characterization of a type III secretion-associated chaperone in the type III secretion system 1 of Vibrio parahaemolyticus (2009)
  • Author(s): Yukihiro Akeda, Kanna Okayama, Tomomi Kimura, Rikard Dryselius, Toshio Kodama, Kazunori Oishi, Tetsuya Iida, Takeshi Honda
  • Journal Title: FEMS Microbiology Letters 296 Pages: 18-25
  • Peer Reviewed
• [Journal Article] Intranasal immunization with a mixture of PspA and a Toll-like receptor agonist induces specific antibodies and enhances bacterial clearance in the airways of mice (2009)
  • Author(s): Keita Oma, Jizi Zhao, Hirokazu Ezoe, Yukihiro Akeda, Shohei Koyama, Ken J. Ishii, Kosuke Kataoka, Kazunori Oishi
  • Journal Title: Vaccine 27 Pages: 3181-3188
  • Peer Reviewed
• [Journal Article] Identification and characterization of a type III secretion-associated chaperone in the type III secretion system 1 of Vibrio parahaemolyticus (2009)
  • Author(s): Y Akeda, K Okayama, T Kimura, R Dryselius, T Kodama, K Oishi, T Iida, T Honda
  • Journal Title: FEMS Microbiology Letters 296 Pages: 18-25
  • Peer Reviewed
• [Journal Article] Intranasal immunization with a mixture of PspA and a Toll-like receptor agonist induces specific antibodies and enhances bacterial clearance in the airways of mice (2009)
  • Author(s): K Oma, J Zhao, H Ezoe, Y Akeda, S Koyama, KJ Ishii, K Kataoka, K Oishi.
  • Journal Title: Vaccine 27 Pages: 3181-3188
  • Peer Reviewed
• [Journal Article] Sixty years of Vibrio parahaemolyticus research (2008)
  • Author(s): Takeshi Honda, Tetsuya Iida, Yukihiro Akeda, Toshio Kodama
  • Journal Title: Microbe 3 Pages: 462-466
• [Journal Article] Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2 (2008)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Kazuyoshi Goto, h, Yukihiro Akeda, Shigeaki Matsuda, Kwon-Sam Park, Vlademir V. Cantarelli, Tetsuya Iida, Takeshi Honda
  • Journal Title: Infection and Immunity 76 Pages: 4282-4289
  • Peer Reviewed
• [Journal Article] 腸管出血性大腸菌O157感染症 (2008)
  • Author(s): 明田幸宏
  • Journal Title: 綜合臨床 57 Pages: 693-2696
• [Journal Article] Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2 (2008)
  • Author(s): T Kodama, H Hiyoshi, K Gotoh, Y Akeda, S Matsuda, KS Park, VV Cantarelli, T Iida, T Honda
  • Journal Title: Infection and Immunity 76 Pages: 4282-4289
  • Peer Reviewed
• [Journal Article] Sixty years of Vibrio parahaemolyticus research (2008)
  • Author(s): T Honda, T Iida, Y Akeda, T Kodama
  • Journal Title: Microbe 3 Pages: 462-466
• [Presentation] Protein secretion systems of Vibrio parahaemolyticus (2010)
  • Author(s): 明田幸宏
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Protection of fatal secondary pneumococcal infection following influenza virus infection by pneumococcal surface protein A (PspA) immunization (2010)
  • Author(s): Hirokazu Ezoe, Yukihihiro Akeda, Kazunori Oishi
  • Organizer: US-Japan Cooperative Medical Science Program Acute Respiratory Infections Panel 2010
  • Place of Presentation: San Francisco, CA USA
• [Presentation] Protection of fatal secondary pneumococcal infection following influenza virus infection by pneumococcal surface protein A (PspA) immunization (2010)
  • Author(s): Hirokazu Ezoe, Yukihiro Akeda, Kazunori Oishi
  • Organizer: US-Japan Cooperative Medical Science Program-Acute Respiratory Infections Panel
  • Place of Presentation: San Francisco, CA, USA
• [Presentation] Protein secretion systems of Vibrio parabaemolyticus (2010)
  • Author(s): 明田幸宏
  • Organizer: 第83日本細胞学会総会
  • Place of Presentation: 横浜
• [Presentation] マウス致死的肺炎モデルを場とした低用量のPspAとTLR ligand併用による経鼻粘膜ワクチンの効果 (2009)
  • Author(s): 朴貞玉、江副浩和、大間敬太、明田幸宏、大石和徳
  • Organizer: 第52回日本感染症学会中日本地方会学術集会・第57回日本化学療法学会西日本支部総会合同集会
  • Place of Presentation: 名古屋
• [Presentation] タイで急増するヒトStreptococcus suis感染症の分子疫学的検討 (2009)
  • Author(s): 大石和徳、内田隆一、明田幸宏
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京
• [Presentation] マウス肺炎モデルを場としたPspAに対するTLR ligandの粘膜アジュバント効果 (2009)
  • Author(s): 江副浩和、大間敬太、明田幸宏、大石和徳
  • Organizer: 第83回日本感染症学会
  • Place of Presentation: 東京
• [Presentation] 腸炎ビブリオT3SS2シャペロンの同定とその機能解析 (2009)
  • Author(s): 明田幸宏、児玉年央、大石和徳、飯田哲也、本田武司
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] マウス肺炎モデルを場としたPspAに対するTLR ligandの粘膜アジュバント効果 (2009)
  • Author(s): 江副浩和、大間敬太、明田幸宏、大石和徳
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京
• [Presentation] マウス致死的肺炎モデルを場とした低用量のPspAとTLR ligand併用による経鼻粘膜ワクチンの効果 (2009)
  • Author(s): 朴貞玉、江副浩和、大間敬太、明田幸宏、大石和徳
  • Organizer: 第52回日本感染症学会中日本地方会学術集会・第57会日本化学療法学会西日本支部総会 合同集会
  • Place of Presentation: 名古屋