Identification and characterization of a new DNA double strand break repair pathway in enterohemorrhagic Escherichia coli

• Project/Area Number: 20790338
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: OOKA Tadasuke University of Miyazaki, 医学部, 助教 (50363594)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 微生物 / 細菌 / ゲノム / 遺伝子

Research Abstract

We identified a new factor that promote the excision of insertion sequence element (IS) in enterohemorrhagic Escherichia coli, and named the protein as IS-excision enhancer (IEE). From the functional analyses, IEE promote the excision of IS3 members from the Escherichia coli O157 genome by combined action with IS transposase. In addition, homologs of IEE were widely distributed in a variety of bacterial species by horizontal gene transfer.

Research Products

• [Journal Article] Identification of candidate pathogens of papillomatous digital dermatitis in dairy cattle from quantitative 16S rRNA clonal analysis. (2010)
  • Author(s): T.Yano, et al.
  • Journal Title: Vet.Microbiol. (in press)
  • Peer Reviewed
• [Journal Article] Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomes (2009)
  • Author(s): T. Ooka, Y. Ogura, Md. Asadulghani, M. Ohnishi, K. Nakayama, J. Terajima, H. Watanabe, T. Hayashi
  • Journal Title: Genome Res. 19 Pages: 1809-1816
  • Peer Reviewed
• [Journal Article] Development of a multiplex PCR-based rapid typing method 1 for enterohemorrhagic Escherichia coli O157 strains (2009)
  • Author(s): T. Ooka, J. Terajima, M. Kusumoto, A. Iguchi, K. Kurokawa, Y. Ogura, M. Asadulghani, K. Nakayama, K. Murase, M. Ohnishi, S. Iyoda, H. Watanabe, T. Hayashi
  • Journal Title: J. Clin. Microbiol 47(9) Pages: 2888-2894
  • Peer Reviewed
• [Journal Article] Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli (2009)
  • Author(s): Y. Ogura, T. Ooka, A. Iguchi, H. Toh, Md. Asadulghani, K. Oshima, T. Kodama, H. Abe, K. Nakayama, K. Kurokawa, T. Tobe, M. Hattori, T. Hayashi
  • Journal Title: Proc. Natl. Acad. Sci. USA 106(42) Pages: 17939-17944
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinants (2009)
  • Author(s): Md. Asadulghani, Y. Ogura, T. Ooka, T. Itoh, A. Sawaguchi, A. Iguchi, K. Nakayama, T. Hayashi
  • Journal Title: PLoS Pathog 5(5)
  • Peer Reviewed
• [Journal Article] Enterohemorrhagic Escherichia coli effector EspL2 induces actin microfilament aggregation through annexin 2 activation (2009)
  • Author(s): A. Miyahara, N. Nakanishi, T. Ooka, T. Hayashi, N. Sugimoto, T. Tobe
  • Journal Title: Cell Microbiol 11(2) Pages: 337-350
  • Peer Reviewed
• [Journal Article] The complete genome sequence and comparative genome analysis of enteropathogenic E.coli O127:H6 strain E2348/69 (2009)
  • Author(s): A. Iguchi, N.R. Thomson, Y. Ogura, D. Saunders, T. Ooka, I.R. Henderson, D. Harris, Asadulghani, K. Kurokawa, P. Dean, B. Kenny, M.A. Quail, S. Thurston, G. Dougan, T. Hayashi, J. Parkhill, *G. Frankel
  • Journal Title: J. Bacteriol. 191(1) Pages: 347-354
  • Peer Reviewed
• [Journal Article] ゲノム解析から見えてきた腸管出血性大腸菌の毒素及び病原因子の新知見 (2009)
  • Author(s): 小椋義俊, 大岡唯祐, 林哲也
  • Journal Title: 化学療法の領域 25(5) Pages: 767-777
• [Journal Article] Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polvmornhisms in Escherichia coli O157 genomes. (2009)
  • Author(s): T.Ooka, et al.
  • Journal Title: Genome Res. 19 Pages: 1809-1816
  • Peer Reviewed
• [Journal Article] Development of a multiplex PCR-based rapid typing method 1 for enterohemorrhagic Escherichia coli O157 strains. (2009)
  • Author(s): T.Ooka, et al.
  • Journal Title: J.Clin.Microbiol. 47(9) Pages: 2888-2894
  • Peer Reviewed
• [Journal Article] Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. (2009)
  • Author(s): Y.Ogura, et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106(42) Pages: 17939-17944
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli O157 : prophage-prophage interactions potentiate horizontal transfer of virulence determinants. (2009)
  • Author(s): Md.Asadulghani, et al.
  • Journal Title: PLoS Pathog. 5(5)
  • Peer Reviewed
• [Journal Article] Enterohemorrhagic Escherichia coli effector EspL2 induces actin micr ofilament aaereaation through annexin 2 activation (2009)
  • Author(s): Miyahara A., et.al.
  • Journal Title: Cell. Microbiol 11(2) Pages: 337-350
  • Peer Reviewed
• [Journal Article] The complete genome sequence and comparative genome analysis of enteropathogenic E. coli 0127 : H6 strain E2348/69 (2009)
  • Author(s): Iguchi A., et.al.
  • Journal Title: J. Bacteriol 191(1) Pages: 347-354
  • Peer Reviewed
• [Journal Article] Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult (2008)
  • Author(s): K. Oshima, H. Toh, Y. Ogura, H. Sasamoto, H. Morita, S. -H. Park, T. Ooka, S. Iyoda, T.D. Taylor, T. Hayashi, K. Itoh, M. Hattori
  • Journal Title: DNA Res 15(6) Pages: 375-386
  • Peer Reviewed
• [Journal Article] Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid mapping (2008)
  • Author(s): Y. Ogura, H. Abe, K. Katsura, K. Kurokawa, Asadulghani, A. Iguchi, T. Ooka, K. Nakayama, A. Yamashita, M. Hattori, T. Tobe, T. Hayashi
  • Journal Title: J. Bacteriol 190(21) Pages: 6948-6960
  • Peer Reviewed
• [Journal Article] The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution. (2008)
  • Author(s): K. Nakayama, A. Yamashita, K. Kurokawa, T. Morimoto, M. Ogawa, M. Fukuhara, H. Urakami, M. Ohnishi, I. Uchiyama, Y. Ogura, T. Ooka, K. Oshima, A. Tamura, M. Hattori, T. Hayashi
  • Journal Title: DNA Res. 15 Pages: 185-199
  • Peer Reviewed
• [Journal Article] Distribution, functional expression genetic organization of Cif, a phageencoded type III-secreted effector from enteropathogenic and enterohaemorraaic Escherichia coli (2008)
  • Author(s): Loukiadis E., et.al.
  • Journal Title: J. Bacteriol 190(1) Pages: 275-285
  • Peer Reviewed
• [Journal Article] Enteropathogenic Escherichia coli (EPEC) 0125 : H6 triggers attaching and effacing lesions on human intestinal biopsies independentlyof Nck (2008)
  • Author(s): Bai L., et.al.
  • Journal Title: Infect. Immun 76(1) Pages: 361-368
  • Peer Reviewed
• [Journal Article] Genomic comparison of the Oantigen biosynthesis gene clusters of Escherichia coli 055 strains belonging to three distinct lineages (2008)
  • Author(s): Iguchi A., et.al.
  • Journal Title: Microbiology 154(2) Pages: 559-570
  • Peer Reviewed
• [Journal Article] Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid manning (2008)
  • Author(s): Ogura Y, et.al.
  • Journal Title: J. Bacteriol 190(21) Pages: 6948-6960
  • Peer Reviewed
• [Journal Article] Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult (2008)
  • Author(s): Oshima K., et.al.
  • Journal Title: DNA Res 15(6) Pages: 375-386
  • Peer Reviewed
• [Journal Article] Identification of candidate pathogens of papillomatous digital dermatitis in dairy cattle from quantitative 16S rRNA clonal analysis
  • Author(s): T. Yano, K.K. Moe, K. Yamazaki, T. Ooka, T. Hayashi, N. Misawa
  • Journal Title: Vet. Microbiol. (in press)
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli 0157 : prophage-prophage interactions potentiate horizontal transfer of virulence deter minants
  • Author(s): Asadulghani, et.al.
  • Journal Title: PLoS Pathog (in press)
  • Peer Reviewed
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)における3型分泌系(T3SS)の進化 (2010)
  • Author(s): 大岡唯祐, 小椋義俊, 中山恵介, 小林秀樹, 林哲也
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] 病原性大腸菌における3型分泌系(T3SS)の進化 (2010)
  • Author(s): 大岡唯祐, 小椋義俊, 中山恵介, 小林秀樹, 林哲也
  • Organizer: 第4回ゲノム微生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 病原性大腸菌における3型分泌系(T3SS)の進化. (2010)
  • Author(s): 大岡唯祐, 他
  • Organizer: 第4回ゲノム微生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)における3型分泌系(T3SS)の進化. (2010)
  • Author(s): 大岡唯祐, 他
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)におけるIII型分泌系コード領域(LEE)の構造比較解析 (2009)
  • Author(s): 大岡唯祐, 小椋義俊, 村瀬一典, Islam Md. Rakibul, 中山恵介, 林哲也
  • Organizer: 若手研究者育成のためのワークショップ～若手コロセウム(III) in宮崎～
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-27
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)におけるIII型分泌系コード領域(LEE)の構造比較解析. (2009)
  • Author(s): 大岡唯祐, 他
  • Organizer: 若手研究者育成のためのワークショップ～若手コロセウム(III)in宮崎～
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-27
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)におけるLEE領域の多様性解析 (2009)
  • Author(s): 大岡唯祐, 小椋義俊, 井口純, Md Asadulghani, 中山恵介, 小林秀樹, 寺嶋淳, 渡邉治雄, 林哲也
  • Organizer: 日本分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)におけるLEE領域の多様性解析. (2009)
  • Author(s): 大岡唯祐, 他
  • Organizer: 日本分子生物学会 第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] 腸管出血性大腸菌(EHEC)及び腸管病原性大腸菌(EPEC)におけるLEE領域の多様性解析 (2009)
  • Author(s): 大岡唯祐, 小椋義俊, 井口純, Asadulghani, 中山恵介, 小林秀樹, 寺嶋淳, 渡邉治雄, 林哲也
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] STECにおいてISの転移に関与する因子の同定 (2009)
  • Author(s): 楠本正博, 大岡唯祐, 西矢芳昭, 小椋義俊, 林哲也
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 病原性大腸菌のIII型分泌システムをコードするLEE領域に関する多様性解析 (2009)
  • Author(s): 大岡唯祐, 小椋義俊, 井口純, Asadulghani, 中山恵介, 小林秀樹, 寺嶋淳, 渡邉治雄, 林哲也
  • Organizer: 第3回ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Remarks] 商品化された製品:O157 IS-printing system ((発売元:TOYOBO)
• [Patent(Industrial Property Rights)] 微生物の識別方法、及び該方法に使用するプライマー並びに識別キット (2006)
  • Inventor(s): 大岡唯祐、林哲也
  • Industrial Property Rights Holder: 大岡唯祐、林哲也
  • Industrial Property Number: 2006-155279
  • Filing Date: 2006-06-02