Identification of genomic region that confers Stx2 producibility of Stx2phages in Escherichia coli O157.
| Project/Area Number |
20790339
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | University of Miyazaki |
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Principal Investigator |
OGURA Yoshitoshi University of Miyazaki, フロンティア科学実験総合センター, 助教 (40363585)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 感染症 / 微生物 / 毒素 / 遺伝子 / ゲノム |
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| Research Abstract |
Enterohemorrhagic Escherichia coli (EHEC) O157 causes severe food-borne illness in humans. The key virulence factor of EHEC is Shiga toxins (Stx1 and Stx2), which are encoded by lambdoid bacteriophages. It is known that producibility of both Stx1 and Stx2 are diversified among strains, but the reasons remain obscure. In this study, in order to reveal the genomic context of the diversity of toxin producibility among O157 strains, we performed fine comparison of Stx phages. Stx1 and Stx2 production levels among O157 strains isolated in Japan were highly divergent. We could not found any correlation between toxin producibility and phylogroups of strains. By comparative genomics, we found high degree of genomic diversity among Stx phage genomes. Now, we are analyzing correlation between toxin producibility and Stx phage types.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli2009
Author(s)
Y. Ogura, T. Ooka, A. Iguchi, H. Toh, Md. Asadulghani, K. Oshima, T. Kodama, H. Abe, K. Nakayama, K. Kurokawa, T. Tobe, M. Hattori, T. Hayashi
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Journal Title
Proc. Natl. Acad. Sci. USA 106(42)
Pages: 17939-17944
Related Report
Peer Reviewed
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[Journal Article] A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis.2009
Author(s)
S.R. Leopold, V. Magrini, N.J. Holt, N. Shaikh, E.R. Mardis, J. Cagno, Y. Ogura, A. Iguchi, T. Hayashi, A. Mellmann, H. Karch, T.E. Besser, S.A. Sawyer, T.S. Whittam, P.I. Tarr
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Journal Title
Proc. Natl. Acad. Sci. USA. 106(21)
Pages: 8713-8718
Related Report
Peer Reviewed
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[Journal Article] Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid mapping2008
Author(s)
Y. Ogura, H. Abe, K. Katsura, K. Kurokawa, Asadulghani, A. Iguchi, T. Ooka, K. Nakayama, A. Yamashita, M. Hattori, T. Tobe, T. Hayashi
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Journal Title
J. Bacteriol. 190(21)
Pages: 6948-6960
Related Report
Peer Reviewed
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